Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland
For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, poss...
Published in: | Polar Biology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://pure.au.dk/portal/da/publications/morphometric-molecular-and-histopathologic-description-of-hepatic-infection-by-orthosplanchnus-arcticus-trematoda-digenea-brachycladiidae-in-ringed-seals-pusa-hispida-from-northwest-greenland(662d750d-04b0-4788-afd8-10c295112e77).html https://doi.org/10.1007/s00300-017-2245-6 http://www.scopus.com/inward/record.url?scp=85040648699&partnerID=8YFLogxK |
id |
ftuniaarhuspubl:oai:pure.atira.dk:publications/662d750d-04b0-4788-afd8-10c295112e77 |
---|---|
record_format |
openpolar |
spelling |
ftuniaarhuspubl:oai:pure.atira.dk:publications/662d750d-04b0-4788-afd8-10c295112e77 2023-05-15T14:31:48+02:00 Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland Andersen-Ranberg, Emilie Ulrikka Lehnert, Kristina Leifsson, Páll S. Dietz, Rune Andersen, Steen Siebert, Ursula Measures, Lena Sonne, Christian 2018-05-01 https://pure.au.dk/portal/da/publications/morphometric-molecular-and-histopathologic-description-of-hepatic-infection-by-orthosplanchnus-arcticus-trematoda-digenea-brachycladiidae-in-ringed-seals-pusa-hispida-from-northwest-greenland(662d750d-04b0-4788-afd8-10c295112e77).html https://doi.org/10.1007/s00300-017-2245-6 http://www.scopus.com/inward/record.url?scp=85040648699&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Andersen-Ranberg , E U , Lehnert , K , Leifsson , P S , Dietz , R , Andersen , S , Siebert , U , Measures , L & Sonne , C 2018 , ' Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland ' , Polar Biology , vol. 41 , no. 5 , pp. 1019-1025 . https://doi.org/10.1007/s00300-017-2245-6 Trematoda Molecular identification PCR morphometrics MT-ND3 Pinniped article 2018 ftuniaarhuspubl https://doi.org/10.1007/s00300-017-2245-6 2023-01-18T23:55:23Z For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses). Article in Journal/Newspaper Arctic monitoring and assessment program Arctic Climate change Greenland Human health Phoca hispida Polar Biology Pusa hispida Qaanaaq Thule Aarhus University: Research Arctic Greenland Qaanaaq ENVELOPE(-69.232,-69.232,77.467,77.467) Polar Biology 41 5 1019 1025 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Trematoda Molecular identification PCR morphometrics MT-ND3 Pinniped |
spellingShingle |
Trematoda Molecular identification PCR morphometrics MT-ND3 Pinniped Andersen-Ranberg, Emilie Ulrikka Lehnert, Kristina Leifsson, Páll S. Dietz, Rune Andersen, Steen Siebert, Ursula Measures, Lena Sonne, Christian Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
topic_facet |
Trematoda Molecular identification PCR morphometrics MT-ND3 Pinniped |
description |
For the first time in > 30 years of routine sampling under the Arctic Monitoring and Assessment Program, a parasite was found in the liver of ringed seals (Phoca hispida) collected near Qaanaaq (Thule), Northwest Greenland, in 2008 and 2014. Concerns regarding changes to parasite occurrence, possibly related to climate change and bioaccumulation of immunomodulating anthropogenic pollutants, spurred further investigations into parasite characterization, and implications for wildlife health and seal hunters. Microscopic, molecular, and morphometric analyses are presented herein. Of 40 seals, 6 (15%) were infected, and 5 of 6 of these seals had severe infections. The parasite was identified morphologically as Orthosplanchnus arcticus Odhner, 1905 (Trematoda; Digenea: Brachycladiidae). Macro- and microscopic pathologic study indicated mild-to-severe biliary hyperplasia associated, stasis associated, and fibrosis associated with trematode infections. Molecular analysis of the trematode confirmed its classification within the Brachycladiidae using sequencing and comparing Internal Transcribed Spacer-1, mitochondrial-NADH Dehydrogenase 3, 18S ribosomal DNA, and Cyclooxygenase-1 regions. This is the first ever report of O. arcticus in ringed seals from Qaanaaq and is one of the most northern records of this parasite. We found significant liver pathology in severely infected seals, but its effects on health of seals in this population are unknown. Host-specificity and the lifecycle of O. arcticus are unknown, but transmission may involve subsistence and commercially harvested Arctic fish species. Further work is needed to answer these questions. Surveying parasites in Arctic wildlife is important in order to assess potential effects on wildlife and human health (i.e., zoonoses). |
format |
Article in Journal/Newspaper |
author |
Andersen-Ranberg, Emilie Ulrikka Lehnert, Kristina Leifsson, Páll S. Dietz, Rune Andersen, Steen Siebert, Ursula Measures, Lena Sonne, Christian |
author_facet |
Andersen-Ranberg, Emilie Ulrikka Lehnert, Kristina Leifsson, Páll S. Dietz, Rune Andersen, Steen Siebert, Ursula Measures, Lena Sonne, Christian |
author_sort |
Andersen-Ranberg, Emilie Ulrikka |
title |
Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
title_short |
Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
title_full |
Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
title_fullStr |
Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
title_full_unstemmed |
Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland |
title_sort |
morphometric, molecular and histopathologic description of hepatic infection by orthosplanchnus arcticus (trematoda: digenea: brachycladiidae) in ringed seals ( pusa hispida ) from northwest greenland |
publishDate |
2018 |
url |
https://pure.au.dk/portal/da/publications/morphometric-molecular-and-histopathologic-description-of-hepatic-infection-by-orthosplanchnus-arcticus-trematoda-digenea-brachycladiidae-in-ringed-seals-pusa-hispida-from-northwest-greenland(662d750d-04b0-4788-afd8-10c295112e77).html https://doi.org/10.1007/s00300-017-2245-6 http://www.scopus.com/inward/record.url?scp=85040648699&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-69.232,-69.232,77.467,77.467) |
geographic |
Arctic Greenland Qaanaaq |
geographic_facet |
Arctic Greenland Qaanaaq |
genre |
Arctic monitoring and assessment program Arctic Climate change Greenland Human health Phoca hispida Polar Biology Pusa hispida Qaanaaq Thule |
genre_facet |
Arctic monitoring and assessment program Arctic Climate change Greenland Human health Phoca hispida Polar Biology Pusa hispida Qaanaaq Thule |
op_source |
Andersen-Ranberg , E U , Lehnert , K , Leifsson , P S , Dietz , R , Andersen , S , Siebert , U , Measures , L & Sonne , C 2018 , ' Morphometric, molecular and histopathologic description of hepatic infection by Orthosplanchnus arcticus (Trematoda: Digenea: Brachycladiidae) in ringed seals ( Pusa hispida ) from Northwest Greenland ' , Polar Biology , vol. 41 , no. 5 , pp. 1019-1025 . https://doi.org/10.1007/s00300-017-2245-6 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00300-017-2245-6 |
container_title |
Polar Biology |
container_volume |
41 |
container_issue |
5 |
container_start_page |
1019 |
op_container_end_page |
1025 |
_version_ |
1766305330745049088 |