Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank

Microbial communities living in deeply buried sediment may be adapted to long-term energy limitation as they are removed from new detrital energy inputs for thousands to millions of years. However, sediment layers near the underlying oceanic crust may receive inputs from below that influence microbi...

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Published in:Frontiers in Microbiology
Main Authors: Labonte, Jessica M., Lever, Mark A., Edwards, Katrina J., Orcutt, Beth N.
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Online Access:https://pure.au.dk/portal/da/publications/influence-of-igneous-basement-on-deep-sediment-microbial-diversity-on-the-eastern-juan-de-fuca-ridge-flank(60643ead-7a52-4acf-be78-a04a12da7765).html
https://doi.org/10.3389/fmicb.2017.01434
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spelling ftuniaarhuspubl:oai:pure.atira.dk:publications/60643ead-7a52-4acf-be78-a04a12da7765 2023-05-15T17:35:15+02:00 Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank Labonte, Jessica M. Lever, Mark A. Edwards, Katrina J. Orcutt, Beth N. 2017-08-02 https://pure.au.dk/portal/da/publications/influence-of-igneous-basement-on-deep-sediment-microbial-diversity-on-the-eastern-juan-de-fuca-ridge-flank(60643ead-7a52-4acf-be78-a04a12da7765).html https://doi.org/10.3389/fmicb.2017.01434 eng eng info:eu-repo/semantics/restrictedAccess Labonte , J M , Lever , M A , Edwards , K J & Orcutt , B N 2017 , ' Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank ' , Frontiers in Microbiology , vol. 8 , 1434 . https://doi.org/10.3389/fmicb.2017.01434 deep biosphere IODP basalt sediment Census of Deep Life MID-ATLANTIC RIDGE SEA-FLOOR SINGLE-CELL OCEAN CRUST SUBSEAFLOOR SEDIMENTS MARINE-SEDIMENTS BASALTIC CRUST NORTH-ATLANTIC WESTERN FLANK FLUIDS article 2017 ftuniaarhuspubl https://doi.org/10.3389/fmicb.2017.01434 2022-12-28T23:54:50Z Microbial communities living in deeply buried sediment may be adapted to long-term energy limitation as they are removed from new detrital energy inputs for thousands to millions of years. However, sediment layers near the underlying oceanic crust may receive inputs from below that influence microbial community structure and/or activity. As part of the Census of Deep Life, we used 16S rRNA gene tag pyrosequencing on DNA extracted from a spectrum of deep sediment-basement interface samples from the subsurface of the Juan de Fuca Ridge flank (collected on IODP Expedition 327) to examine this possible basement influence on deep sediment communities. This area experiences rapid sedimentation, with an underlying basaltic crust that hosts a dynamic flux of hydrothermal fluids that diffuse into the sediment. Chloroflexi sequences dominated tag libraries in all sediment samples, with variation in the abundance of other bacterial groups (e.g., Actinobacteria, Aerophobetes, Atribacteria, Planctomycetes, and Nitrospirae). These variations occur in relation to the type of sediment (clays versus carbonate-rich) and the depth of sample origin, and show no clear connection to the distance from the discharge outcrop or to basement fluid microbial communities. Actinobacteria-related sequences dominated the basalt libraries, but these should be viewed cautiously due to possibilities for imprinting from contamination. Our results indicate that proximity to basement or areas of seawater recharge is not a primary driver of microbial community composition in basal sediment, even though fluids diffusing from basement into sediment may stimulate microbial activity. Article in Journal/Newspaper North Atlantic Aarhus University: Research Mid-Atlantic Ridge Frontiers in Microbiology 8
institution Open Polar
collection Aarhus University: Research
op_collection_id ftuniaarhuspubl
language English
topic deep biosphere
IODP
basalt
sediment
Census of Deep Life
MID-ATLANTIC RIDGE
SEA-FLOOR
SINGLE-CELL
OCEAN CRUST
SUBSEAFLOOR SEDIMENTS
MARINE-SEDIMENTS
BASALTIC CRUST
NORTH-ATLANTIC
WESTERN FLANK
FLUIDS
spellingShingle deep biosphere
IODP
basalt
sediment
Census of Deep Life
MID-ATLANTIC RIDGE
SEA-FLOOR
SINGLE-CELL
OCEAN CRUST
SUBSEAFLOOR SEDIMENTS
MARINE-SEDIMENTS
BASALTIC CRUST
NORTH-ATLANTIC
WESTERN FLANK
FLUIDS
Labonte, Jessica M.
Lever, Mark A.
Edwards, Katrina J.
Orcutt, Beth N.
Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
topic_facet deep biosphere
IODP
basalt
sediment
Census of Deep Life
MID-ATLANTIC RIDGE
SEA-FLOOR
SINGLE-CELL
OCEAN CRUST
SUBSEAFLOOR SEDIMENTS
MARINE-SEDIMENTS
BASALTIC CRUST
NORTH-ATLANTIC
WESTERN FLANK
FLUIDS
description Microbial communities living in deeply buried sediment may be adapted to long-term energy limitation as they are removed from new detrital energy inputs for thousands to millions of years. However, sediment layers near the underlying oceanic crust may receive inputs from below that influence microbial community structure and/or activity. As part of the Census of Deep Life, we used 16S rRNA gene tag pyrosequencing on DNA extracted from a spectrum of deep sediment-basement interface samples from the subsurface of the Juan de Fuca Ridge flank (collected on IODP Expedition 327) to examine this possible basement influence on deep sediment communities. This area experiences rapid sedimentation, with an underlying basaltic crust that hosts a dynamic flux of hydrothermal fluids that diffuse into the sediment. Chloroflexi sequences dominated tag libraries in all sediment samples, with variation in the abundance of other bacterial groups (e.g., Actinobacteria, Aerophobetes, Atribacteria, Planctomycetes, and Nitrospirae). These variations occur in relation to the type of sediment (clays versus carbonate-rich) and the depth of sample origin, and show no clear connection to the distance from the discharge outcrop or to basement fluid microbial communities. Actinobacteria-related sequences dominated the basalt libraries, but these should be viewed cautiously due to possibilities for imprinting from contamination. Our results indicate that proximity to basement or areas of seawater recharge is not a primary driver of microbial community composition in basal sediment, even though fluids diffusing from basement into sediment may stimulate microbial activity.
format Article in Journal/Newspaper
author Labonte, Jessica M.
Lever, Mark A.
Edwards, Katrina J.
Orcutt, Beth N.
author_facet Labonte, Jessica M.
Lever, Mark A.
Edwards, Katrina J.
Orcutt, Beth N.
author_sort Labonte, Jessica M.
title Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
title_short Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
title_full Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
title_fullStr Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
title_full_unstemmed Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank
title_sort influence of igneous basement on deep sediment microbial diversity on the eastern juan de fuca ridge flank
publishDate 2017
url https://pure.au.dk/portal/da/publications/influence-of-igneous-basement-on-deep-sediment-microbial-diversity-on-the-eastern-juan-de-fuca-ridge-flank(60643ead-7a52-4acf-be78-a04a12da7765).html
https://doi.org/10.3389/fmicb.2017.01434
geographic Mid-Atlantic Ridge
geographic_facet Mid-Atlantic Ridge
genre North Atlantic
genre_facet North Atlantic
op_source Labonte , J M , Lever , M A , Edwards , K J & Orcutt , B N 2017 , ' Influence of Igneous Basement on Deep Sediment Microbial Diversity on the Eastern Juan de Fuca Ridge Flank ' , Frontiers in Microbiology , vol. 8 , 1434 . https://doi.org/10.3389/fmicb.2017.01434
op_rights info:eu-repo/semantics/restrictedAccess
op_doi https://doi.org/10.3389/fmicb.2017.01434
container_title Frontiers in Microbiology
container_volume 8
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