Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis
Arcellinida (testate lobose amoebae) are widely used as bio-indicators of lacustrine environmental change. Too much obscuring organic material in a gridded wet Petri dish preparation makes it difficult to observe all specimens present and slows quantification as the organic material has to be carefu...
Published in: | Microbial Ecology |
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2020
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ftuniaarhuspubl:oai:pure.atira.dk:publications/5df9adf4-cd33-4143-934e-db3ec7cb957e 2023-05-15T17:46:45+02:00 Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis Nasser, Nawaf A. Gregory, Braden R.B. Steele, Riley E. Patterson, R. Timothy Galloway, Jennifer M. 2020 https://pure.au.dk/portal/da/publications/behind-the-organic-veil(5df9adf4-cd33-4143-934e-db3ec7cb957e).html https://doi.org/10.1007/s00248-019-01429-0 http://www.scopus.com/inward/record.url?scp=85071172131&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Nasser , N A , Gregory , B R B , Steele , R E , Patterson , R T & Galloway , J M 2020 , ' Behind the Organic Veil : Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis ' , Microbial Ecology , vol. 79 , no. 2 , pp. 443-458 . https://doi.org/10.1007/s00248-019-01429-0 Arcellinida Arcellinidan analysis Chemical treatment Lake sediments Organic content Sample preparation methods article 2020 ftuniaarhuspubl https://doi.org/10.1007/s00248-019-01429-0 2020-07-18T22:21:35Z Arcellinida (testate lobose amoebae) are widely used as bio-indicators of lacustrine environmental change. Too much obscuring organic material in a gridded wet Petri dish preparation makes it difficult to observe all specimens present and slows quantification as the organic material has to be carefully worked through with a dissection probe. Chemical deflocculation using soda ash (Na 2 CO 3 ·H 2 O), potassium hydroxide (KOH), or sodium hexametaphosphate ((NaPO 3 ) 6 ) has previously been shown to disaggregate and reduce organic content in lake sediments, but to date, no attempt has been made to comparatively evaluate the efficiency of these deflocculants in disaggregating organic content and their impact on Arcellinida analysis in lacustrine sediments. Here, we assess the effectiveness of soda ash, potassium hydroxide, and sodium hexametaphosphate treatments on removing organic content and the impact of those digestions on Arcellinida preservation in 126 sample aliquots subdivided from three sediment samples (YK-20, YK-25, and YK-57) collected from three lakes near Yellowknife, Northwest Territories, Canada. Following treatment, cluster analysis and Bray-Curtis dissimilarity matrix (BCDM) were utilized to determine whether treatments resulted in dissolution-driven changes in Arcellinida assemblage composition. Observed Arcellinida tests in aliquots increased drastically after treatment of organic-rich samples (47.5–452.7% in organic-rich aliquots and by 14.8% in aliquots with less organic matter). The BCDM results revealed that treatment with 5% KOH resulted in the highest reduction in observed organic content without significantly affecting Arcellinida assemblage structure, while soda ash and sodium hexametaphosphate treatments resulted in marginal organic matter reduction and caused severe damage to the arcellinidan tests. Article in Journal/Newspaper Northwest Territories Yellowknife Aarhus University: Research Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) Canada Northwest Territories Yellowknife Microbial Ecology 79 2 443 458 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Arcellinida Arcellinidan analysis Chemical treatment Lake sediments Organic content Sample preparation methods |
spellingShingle |
Arcellinida Arcellinidan analysis Chemical treatment Lake sediments Organic content Sample preparation methods Nasser, Nawaf A. Gregory, Braden R.B. Steele, Riley E. Patterson, R. Timothy Galloway, Jennifer M. Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
topic_facet |
Arcellinida Arcellinidan analysis Chemical treatment Lake sediments Organic content Sample preparation methods |
description |
Arcellinida (testate lobose amoebae) are widely used as bio-indicators of lacustrine environmental change. Too much obscuring organic material in a gridded wet Petri dish preparation makes it difficult to observe all specimens present and slows quantification as the organic material has to be carefully worked through with a dissection probe. Chemical deflocculation using soda ash (Na 2 CO 3 ·H 2 O), potassium hydroxide (KOH), or sodium hexametaphosphate ((NaPO 3 ) 6 ) has previously been shown to disaggregate and reduce organic content in lake sediments, but to date, no attempt has been made to comparatively evaluate the efficiency of these deflocculants in disaggregating organic content and their impact on Arcellinida analysis in lacustrine sediments. Here, we assess the effectiveness of soda ash, potassium hydroxide, and sodium hexametaphosphate treatments on removing organic content and the impact of those digestions on Arcellinida preservation in 126 sample aliquots subdivided from three sediment samples (YK-20, YK-25, and YK-57) collected from three lakes near Yellowknife, Northwest Territories, Canada. Following treatment, cluster analysis and Bray-Curtis dissimilarity matrix (BCDM) were utilized to determine whether treatments resulted in dissolution-driven changes in Arcellinida assemblage composition. Observed Arcellinida tests in aliquots increased drastically after treatment of organic-rich samples (47.5–452.7% in organic-rich aliquots and by 14.8% in aliquots with less organic matter). The BCDM results revealed that treatment with 5% KOH resulted in the highest reduction in observed organic content without significantly affecting Arcellinida assemblage structure, while soda ash and sodium hexametaphosphate treatments resulted in marginal organic matter reduction and caused severe damage to the arcellinidan tests. |
format |
Article in Journal/Newspaper |
author |
Nasser, Nawaf A. Gregory, Braden R.B. Steele, Riley E. Patterson, R. Timothy Galloway, Jennifer M. |
author_facet |
Nasser, Nawaf A. Gregory, Braden R.B. Steele, Riley E. Patterson, R. Timothy Galloway, Jennifer M. |
author_sort |
Nasser, Nawaf A. |
title |
Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
title_short |
Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
title_full |
Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
title_fullStr |
Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
title_full_unstemmed |
Behind the Organic Veil:Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis |
title_sort |
behind the organic veil:assessing the impact of chemical deflocculation on organic content reduction and lacustrine arcellinida (testate amoebae) analysis |
publishDate |
2020 |
url |
https://pure.au.dk/portal/da/publications/behind-the-organic-veil(5df9adf4-cd33-4143-934e-db3ec7cb957e).html https://doi.org/10.1007/s00248-019-01429-0 http://www.scopus.com/inward/record.url?scp=85071172131&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Bray Canada Northwest Territories Yellowknife |
geographic_facet |
Bray Canada Northwest Territories Yellowknife |
genre |
Northwest Territories Yellowknife |
genre_facet |
Northwest Territories Yellowknife |
op_source |
Nasser , N A , Gregory , B R B , Steele , R E , Patterson , R T & Galloway , J M 2020 , ' Behind the Organic Veil : Assessing the Impact of Chemical Deflocculation on Organic Content Reduction and Lacustrine Arcellinida (Testate Amoebae) Analysis ' , Microbial Ecology , vol. 79 , no. 2 , pp. 443-458 . https://doi.org/10.1007/s00248-019-01429-0 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00248-019-01429-0 |
container_title |
Microbial Ecology |
container_volume |
79 |
container_issue |
2 |
container_start_page |
443 |
op_container_end_page |
458 |
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1766150587308572672 |