Demequina lutea sp. nov., isolated from a high Arctic permafrost soil.

• Published in: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
• Main Authors: Finster, Kai, Herbert, Rodney Andrew, Kjeldsen, Kasper Urup, Schumann, Peter, Lomstein, Bente Aagaard
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2009
• Subjects: Adventdalen; Arctic; Norway; Northern Norway; permafrost; Spitsbergen
• Online Access: https://pure.au.dk/portal/en/publications/562d4a50-3af9-11de-8dc9-000ea68e967b; https://doi.org/10.1099/ijs.0.004929-0

Udgivelsesdato: 2009 Two Gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains SV45 T and SV47), isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway, have been characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder Micrococcineae of the order Actinomycetales . DNA-DNA hybridization analyses indicate that strains SV45 T and SV47 are closely related (60-69 % relatedness) and belong to the same species, although they show slightly different colony pigmentation. The closest phylogenetic neighbour was Demequina aestuarii JC2054 T , with 96 % 16S rRNA gene sequence similarity. Optimum growth of SV45 T and SV47 occurred aerobically in the absence of NaCl, but both isolates tolerated up to 2 % NaCl (w/v) in the growth medium. Growth under anaerobic conditions was slow and weak. The peptidoglycan of both isolates was of the A4 β type with L-ornithine as the diamino acid and serine as a component of the interpeptide bridge with either D-aspartate (SV45 T ) or D-glutamate (SV47) as the N-terminal amino acid. The major fatty acids present in both isolates were C 15 : 0 (3.2-8.6 %), iso-C 16 : 0 (5.0-8.9 %), anteiso-C 15 : 0 (59.4-61.5 %), anteiso-C 17 : 0 (4.1-8.8 %) and anteiso-C 15 : 1 (4.4-6.4 %). Isoprenoid quinones were present at exceptionally low levels in both isolates, and only demethylmenaquinone DMK-9(H 4 ) could be identified with any degree of confidence. Phylogenetic analysis and differences in physiological and biochemical characteristics between the strains and Demequina aestuarii JC2054 T indicate that these isolates belong to a novel species within the genus Demequina , for which the name Demequina lutea sp. nov. is proposed. The type strain is SV45 T (=LMG 24795 T =DSM 19970 T ).