Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet
Weddell (Leptonychotes weddellii), Ross (Ommatophoca rossii) and crabeater seals (Lobodon carcinophaga) are phocid seals with a circumpolar distribution around Antarctica. As long-lived and large top predators, they bioaccumulate contaminants and are considered as sentinels of ecosystem health. Anta...
Published in: | Science of The Total Environment |
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Language: | English |
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2017
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ftuniaarhuspubl:oai:pure.atira.dk:publications/4671ed4a-523f-4dec-bce1-4731e463c6dc 2023-05-15T13:53:30+02:00 Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet Lehnert, K. Weirup, L. Harding, K. C. Harkonen, T. Karisson, O. Teilmann, J. 2017-12-01 https://pure.au.dk/portal/da/publications/antarctic-seals(4671ed4a-523f-4dec-bce1-4731e463c6dc).html https://doi.org/10.1016/j.scitotenv.2017.04.224 eng eng info:eu-repo/semantics/restrictedAccess Lehnert , K , Weirup , L , Harding , K C , Harkonen , T , Karisson , O & Teilmann , J 2017 , ' Antarctic seals : Molecular biomarkers as indicators for pollutant exposure, health effects and diet ' , Science of the Total Environment , vol. 599 , pp. 1693-1704 . https://doi.org/10.1016/j.scitotenv.2017.04.224 mRNA transcription Xenobiotics Cytoldnes Heatshockprotein Mercury Antarctica KRILL EUPHAUSIA-SUPERBA HEAT-SHOCK PROTEINS POLYBROMINATED DIPHENYL ETHERS PERSISTENT ORGANIC POLLUTANTS PORPOISES PHOCOENA-PHOCOENA ARYL-HYDROCARBON RECEPTOR MESSENGER-RNA EXPRESSION CRAB-EATER SEALS QUEEN-MAUD-LAND HARBOR SEALS article 2017 ftuniaarhuspubl https://doi.org/10.1016/j.scitotenv.2017.04.224 2023-01-11T23:55:01Z Weddell (Leptonychotes weddellii), Ross (Ommatophoca rossii) and crabeater seals (Lobodon carcinophaga) are phocid seals with a circumpolar distribution around Antarctica. As long-lived and large top predators, they bioaccumulate contaminants and are considered as sentinels of ecosystem health. Antarctic seals are increasingly exposed to climate change, pollution, shipping and fisheries. To reveal and understand possible anthropogenic impacts on their immune and health status, this study investigates sensitive biomarkers of the xenobiotic metabolism and immune system in relation to mercury (Hg) burden. Gene-transcription studies using minimally invasive blood samples are useful to monitor physiological processes in wildlife that can be related to different stressors. Blood samples of 72 wild-caught seals (Weddell n = 33; Ross n = 12; crabeater n = 27) in the Amundsen and Ross Seas in 2008-2011 were investigated. Copy numbers per mu l mRNA transcription of xenobiotic biomarkers (aryl hydrocarbon receptor (AHR)), aryl hydrocarbon receptor nuclear translocator (ARNT) and peroxisome proliferator-activated receptor (PPAR alpha) and immune relevant cell mediators (cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and heat-shock-protein 70 (HSP70)) were measured using reference genes Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) and ribosomal protein L4 (RPL4) by real time RT-qPCR. Hg concentration was analysed in fur. Hg concentration increased with body weight and standard length in all species. Crabeater seals showed a lower Hg concentration than Ross and Weddell seals. Species-specific differences in gene-transcription were found between all species with highest levels of AHR, ARNT and PPARa in crabeater seals. Ross seals showed highest IL-10 and HSP70 transcription, while HSP70 was exceptionally low in crabeater seals. Between Hg and HSP70 a clear negative relationship was found in all species. The species-specific, age and sex-dependent gene-transcription ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Crabeater Seals Euphausia superba Phocoena phocoena Queen Maud Land Weddell Seals Aarhus University: Research Antarctic Queen Maud Land ENVELOPE(12.000,12.000,-72.500,-72.500) Weddell Science of The Total Environment 599-600 1693 1704 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
mRNA transcription Xenobiotics Cytoldnes Heatshockprotein Mercury Antarctica KRILL EUPHAUSIA-SUPERBA HEAT-SHOCK PROTEINS POLYBROMINATED DIPHENYL ETHERS PERSISTENT ORGANIC POLLUTANTS PORPOISES PHOCOENA-PHOCOENA ARYL-HYDROCARBON RECEPTOR MESSENGER-RNA EXPRESSION CRAB-EATER SEALS QUEEN-MAUD-LAND HARBOR SEALS |
spellingShingle |
mRNA transcription Xenobiotics Cytoldnes Heatshockprotein Mercury Antarctica KRILL EUPHAUSIA-SUPERBA HEAT-SHOCK PROTEINS POLYBROMINATED DIPHENYL ETHERS PERSISTENT ORGANIC POLLUTANTS PORPOISES PHOCOENA-PHOCOENA ARYL-HYDROCARBON RECEPTOR MESSENGER-RNA EXPRESSION CRAB-EATER SEALS QUEEN-MAUD-LAND HARBOR SEALS Lehnert, K. Weirup, L. Harding, K. C. Harkonen, T. Karisson, O. Teilmann, J. Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
topic_facet |
mRNA transcription Xenobiotics Cytoldnes Heatshockprotein Mercury Antarctica KRILL EUPHAUSIA-SUPERBA HEAT-SHOCK PROTEINS POLYBROMINATED DIPHENYL ETHERS PERSISTENT ORGANIC POLLUTANTS PORPOISES PHOCOENA-PHOCOENA ARYL-HYDROCARBON RECEPTOR MESSENGER-RNA EXPRESSION CRAB-EATER SEALS QUEEN-MAUD-LAND HARBOR SEALS |
description |
Weddell (Leptonychotes weddellii), Ross (Ommatophoca rossii) and crabeater seals (Lobodon carcinophaga) are phocid seals with a circumpolar distribution around Antarctica. As long-lived and large top predators, they bioaccumulate contaminants and are considered as sentinels of ecosystem health. Antarctic seals are increasingly exposed to climate change, pollution, shipping and fisheries. To reveal and understand possible anthropogenic impacts on their immune and health status, this study investigates sensitive biomarkers of the xenobiotic metabolism and immune system in relation to mercury (Hg) burden. Gene-transcription studies using minimally invasive blood samples are useful to monitor physiological processes in wildlife that can be related to different stressors. Blood samples of 72 wild-caught seals (Weddell n = 33; Ross n = 12; crabeater n = 27) in the Amundsen and Ross Seas in 2008-2011 were investigated. Copy numbers per mu l mRNA transcription of xenobiotic biomarkers (aryl hydrocarbon receptor (AHR)), aryl hydrocarbon receptor nuclear translocator (ARNT) and peroxisome proliferator-activated receptor (PPAR alpha) and immune relevant cell mediators (cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and heat-shock-protein 70 (HSP70)) were measured using reference genes Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ) and ribosomal protein L4 (RPL4) by real time RT-qPCR. Hg concentration was analysed in fur. Hg concentration increased with body weight and standard length in all species. Crabeater seals showed a lower Hg concentration than Ross and Weddell seals. Species-specific differences in gene-transcription were found between all species with highest levels of AHR, ARNT and PPARa in crabeater seals. Ross seals showed highest IL-10 and HSP70 transcription, while HSP70 was exceptionally low in crabeater seals. Between Hg and HSP70 a clear negative relationship was found in all species. The species-specific, age and sex-dependent gene-transcription ... |
format |
Article in Journal/Newspaper |
author |
Lehnert, K. Weirup, L. Harding, K. C. Harkonen, T. Karisson, O. Teilmann, J. |
author_facet |
Lehnert, K. Weirup, L. Harding, K. C. Harkonen, T. Karisson, O. Teilmann, J. |
author_sort |
Lehnert, K. |
title |
Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
title_short |
Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
title_full |
Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
title_fullStr |
Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
title_full_unstemmed |
Antarctic seals:Molecular biomarkers as indicators for pollutant exposure, health effects and diet |
title_sort |
antarctic seals:molecular biomarkers as indicators for pollutant exposure, health effects and diet |
publishDate |
2017 |
url |
https://pure.au.dk/portal/da/publications/antarctic-seals(4671ed4a-523f-4dec-bce1-4731e463c6dc).html https://doi.org/10.1016/j.scitotenv.2017.04.224 |
long_lat |
ENVELOPE(12.000,12.000,-72.500,-72.500) |
geographic |
Antarctic Queen Maud Land Weddell |
geographic_facet |
Antarctic Queen Maud Land Weddell |
genre |
Antarc* Antarctic Antarctica Crabeater Seals Euphausia superba Phocoena phocoena Queen Maud Land Weddell Seals |
genre_facet |
Antarc* Antarctic Antarctica Crabeater Seals Euphausia superba Phocoena phocoena Queen Maud Land Weddell Seals |
op_source |
Lehnert , K , Weirup , L , Harding , K C , Harkonen , T , Karisson , O & Teilmann , J 2017 , ' Antarctic seals : Molecular biomarkers as indicators for pollutant exposure, health effects and diet ' , Science of the Total Environment , vol. 599 , pp. 1693-1704 . https://doi.org/10.1016/j.scitotenv.2017.04.224 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2017.04.224 |
container_title |
Science of The Total Environment |
container_volume |
599-600 |
container_start_page |
1693 |
op_container_end_page |
1704 |
_version_ |
1766258673495048192 |