Potential microbial contamination during sampling of permafrost soil assessed by tracers
Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biom...
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ftuniaarhuspubl:oai:pure.atira.dk:publications/3ce389d1-1d28-47ed-9c2d-ab55590f3da5 2023-08-27T04:11:28+02:00 Potential microbial contamination during sampling of permafrost soil assessed by tracers Bang-Andreasen, Toke Schostag, Morten Priemé, Anders Elberling, Bo Jacobsen, Carsten S 2017-02-23 application/pdf https://pure.au.dk/portal/da/publications/potential-microbial-contamination-during-sampling-of-permafrost-soil-assessed-by-tracers(3ce389d1-1d28-47ed-9c2d-ab55590f3da5).html https://doi.org/10.1038/srep43338 https://pure.au.dk/ws/files/118911271/srep43338.pdf eng eng info:eu-repo/semantics/openAccess Bang-Andreasen , T , Schostag , M , Priemé , A , Elberling , B & Jacobsen , C S 2017 , ' Potential microbial contamination during sampling of permafrost soil assessed by tracers ' , Scientific Reports , vol. 7 , pp. 43338 . https://doi.org/10.1038/srep43338 Journal Article article 2017 ftuniaarhuspubl https://doi.org/10.1038/srep43338 2023-08-09T22:57:50Z Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores. Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. ... Article in Journal/Newspaper permafrost Aarhus University: Research Scientific Reports 7 1 |
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Aarhus University: Research |
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ftuniaarhuspubl |
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English |
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Journal Article |
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Journal Article Bang-Andreasen, Toke Schostag, Morten Priemé, Anders Elberling, Bo Jacobsen, Carsten S Potential microbial contamination during sampling of permafrost soil assessed by tracers |
topic_facet |
Journal Article |
description |
Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores. Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. ... |
format |
Article in Journal/Newspaper |
author |
Bang-Andreasen, Toke Schostag, Morten Priemé, Anders Elberling, Bo Jacobsen, Carsten S |
author_facet |
Bang-Andreasen, Toke Schostag, Morten Priemé, Anders Elberling, Bo Jacobsen, Carsten S |
author_sort |
Bang-Andreasen, Toke |
title |
Potential microbial contamination during sampling of permafrost soil assessed by tracers |
title_short |
Potential microbial contamination during sampling of permafrost soil assessed by tracers |
title_full |
Potential microbial contamination during sampling of permafrost soil assessed by tracers |
title_fullStr |
Potential microbial contamination during sampling of permafrost soil assessed by tracers |
title_full_unstemmed |
Potential microbial contamination during sampling of permafrost soil assessed by tracers |
title_sort |
potential microbial contamination during sampling of permafrost soil assessed by tracers |
publishDate |
2017 |
url |
https://pure.au.dk/portal/da/publications/potential-microbial-contamination-during-sampling-of-permafrost-soil-assessed-by-tracers(3ce389d1-1d28-47ed-9c2d-ab55590f3da5).html https://doi.org/10.1038/srep43338 https://pure.au.dk/ws/files/118911271/srep43338.pdf |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Bang-Andreasen , T , Schostag , M , Priemé , A , Elberling , B & Jacobsen , C S 2017 , ' Potential microbial contamination during sampling of permafrost soil assessed by tracers ' , Scientific Reports , vol. 7 , pp. 43338 . https://doi.org/10.1038/srep43338 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/srep43338 |
container_title |
Scientific Reports |
container_volume |
7 |
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1 |
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1775354278149357568 |