Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approach...
Published in: | FEMS Microbiology Ecology |
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Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2023
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Online Access: | https://pure.au.dk/portal/da/publications/exploring-microbial-diversity-in-greenland-ice-sheet-supraglacial-habitats-through-culturingdependent-and-independent-approaches(37c57e13-0a2a-4447-9c13-7ee07d2b4a5b).html https://doi.org/10.1093/femsec/fiad119 http://www.scopus.com/inward/record.url?scp=85174751393&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/37c57e13-0a2a-4447-9c13-7ee07d2b4a5b 2023-12-31T10:07:21+01:00 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches Jaarsma, Ate H. Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane Schmidt Stougaard, Peter Benning, Liane G. Tranter, Martyn Anesio, Alexandre M. 2023-11 https://pure.au.dk/portal/da/publications/exploring-microbial-diversity-in-greenland-ice-sheet-supraglacial-habitats-through-culturingdependent-and-independent-approaches(37c57e13-0a2a-4447-9c13-7ee07d2b4a5b).html https://doi.org/10.1093/femsec/fiad119 http://www.scopus.com/inward/record.url?scp=85174751393&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/da/publications/exploring-microbial-diversity-in-greenland-ice-sheet-supraglacial-habitats-through-culturingdependent-and-independent-approaches(37c57e13-0a2a-4447-9c13-7ee07d2b4a5b).html info:eu-repo/semantics/openAccess Jaarsma , A H , Sipes , K , Zervas , A , Jiménez , F C , Ellegaard-Jensen , L , Thøgersen , M S , Stougaard , P , Benning , L G , Tranter , M & Anesio , A M 2023 , ' Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches ' , FEMS Microbiology Ecology , vol. 99 , no. 11 , fiad119 . https://doi.org/10.1093/femsec/fiad119 in situ culturing amplicons Greenland Ice Sheet isolates MAGs metagenome article 2023 ftuniaarhuspubl https://doi.org/10.1093/femsec/fiad119 2023-12-07T00:06:44Z The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. Article in Journal/Newspaper Greenland Ice Sheet Aarhus University: Research FEMS Microbiology Ecology 99 11 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
in situ culturing amplicons Greenland Ice Sheet isolates MAGs metagenome |
spellingShingle |
in situ culturing amplicons Greenland Ice Sheet isolates MAGs metagenome Jaarsma, Ate H. Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane Schmidt Stougaard, Peter Benning, Liane G. Tranter, Martyn Anesio, Alexandre M. Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
topic_facet |
in situ culturing amplicons Greenland Ice Sheet isolates MAGs metagenome |
description |
The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. |
format |
Article in Journal/Newspaper |
author |
Jaarsma, Ate H. Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane Schmidt Stougaard, Peter Benning, Liane G. Tranter, Martyn Anesio, Alexandre M. |
author_facet |
Jaarsma, Ate H. Sipes, Katie Zervas, Athanasios Jiménez, Francisco Campuzano Ellegaard-Jensen, Lea Thøgersen, Mariane Schmidt Stougaard, Peter Benning, Liane G. Tranter, Martyn Anesio, Alexandre M. |
author_sort |
Jaarsma, Ate H. |
title |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_short |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_fullStr |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full_unstemmed |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_sort |
exploring microbial diversity in greenland ice sheet supraglacial habitats through culturing-dependent and -independent approaches |
publishDate |
2023 |
url |
https://pure.au.dk/portal/da/publications/exploring-microbial-diversity-in-greenland-ice-sheet-supraglacial-habitats-through-culturingdependent-and-independent-approaches(37c57e13-0a2a-4447-9c13-7ee07d2b4a5b).html https://doi.org/10.1093/femsec/fiad119 http://www.scopus.com/inward/record.url?scp=85174751393&partnerID=8YFLogxK |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
Jaarsma , A H , Sipes , K , Zervas , A , Jiménez , F C , Ellegaard-Jensen , L , Thøgersen , M S , Stougaard , P , Benning , L G , Tranter , M & Anesio , A M 2023 , ' Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches ' , FEMS Microbiology Ecology , vol. 99 , no. 11 , fiad119 . https://doi.org/10.1093/femsec/fiad119 |
op_relation |
https://pure.au.dk/portal/da/publications/exploring-microbial-diversity-in-greenland-ice-sheet-supraglacial-habitats-through-culturingdependent-and-independent-approaches(37c57e13-0a2a-4447-9c13-7ee07d2b4a5b).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1093/femsec/fiad119 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
11 |
_version_ |
1786839704380899328 |