A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues
In vitro investigations of the health impact of individual chemical compounds have traditionally been used in risk assessments. However, humans and wildlife are exposed to a plethora of potentially harmful chemicals, including organohalogen contaminants (OHCs). An alternative exposure approach to in...
Published in: | Chemosphere |
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2017
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Online Access: | https://pure.au.dk/portal/da/publications/a-rapid-analytical-method-to-quantify-complex-organohalogen-contaminant-mixtures-in-large-samples-of-high-lipid-mammalian-tissues(317f8660-8cf7-4b17-8b71-07652b7900c2).html https://doi.org/10.1016/j.chemosphere.2017.02.098 |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/317f8660-8cf7-4b17-8b71-07652b7900c2 2023-05-15T17:03:40+02:00 A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues Desforges, Jean-Pierre Eulaers, Igor Periard, Luke Sonne, Christian Dietz, Rune Letcher, Robert J. 2017-06 https://pure.au.dk/portal/da/publications/a-rapid-analytical-method-to-quantify-complex-organohalogen-contaminant-mixtures-in-large-samples-of-high-lipid-mammalian-tissues(317f8660-8cf7-4b17-8b71-07652b7900c2).html https://doi.org/10.1016/j.chemosphere.2017.02.098 eng eng info:eu-repo/semantics/restrictedAccess Desforges , J-P , Eulaers , I , Periard , L , Sonne , C , Dietz , R & Letcher , R J 2017 , ' A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues ' , Chemosphere , vol. 176 , pp. 243-248 . https://doi.org/10.1016/j.chemosphere.2017.02.098 Mixture toxicology Realistic exposure Freeze-lipid filtration Marine mammal Polychlorinated biphenyls Flame retardants SOLID-PHASE EXTRACTION 3 DECADES 1983-2010 ACTING CHEMICALS VIBRIO-FISCHERI HARBOR SEALS TOXICITY PREDICTABILITY CHROMATOGRAPHY FILTRATION CLEANUP article 2017 ftuniaarhuspubl https://doi.org/10.1016/j.chemosphere.2017.02.098 2020-07-18T21:58:19Z In vitro investigations of the health impact of individual chemical compounds have traditionally been used in risk assessments. However, humans and wildlife are exposed to a plethora of potentially harmful chemicals, including organohalogen contaminants (OHCs). An alternative exposure approach to individual or simple mixtures of synthetic OHCs is to isolate the complex mixture present in free-ranging wildlife, often non-destructively sampled from lipid rich adipose. High concentration stock volumes required for in vitro investigations do, however, pose a great analytical challenge to extract sufficient amounts of complex OHC cocktails. Here we describe a novel method to easily, rapidly and efficiently extract an environmentally accumulated and therefore relevant contaminant cocktail from large (10-50 g) marine mammal blubber samples. We demonstrate that lipid freeze-filtration with acetonitrile removes up to 97% of blubber lipids, with minimal effect on the efficiency of OHC recovery. Sample extracts after freeze-filtration were further processed to remove residual trace lipids via high-pressure gel permeation chromatography and solid phase extraction. Average recoveries of OHCs from triplicate analysis of killer whale (Orcinus orca), polar bear (Ursus maritimus) and pilot whale (Globicephala spp.) blubber standard reference material (NIST SRM-1945) ranged from 68 to 80%, 54-92% and 58-145%, respectively, for C-13-enriched internal standards of six polychlorinated biphenyl congeners, 16 organochlorine pesticides and four brominated flame retardants. This approach to rapidly generate OHC mixtures shows great potential for experimental exposures using complex contaminant mixtures, research or monitoring driven contaminant quantification in biological samples, as well as the untargeted identification of emerging contaminants. (C) 2017 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Killer Whale Orca Orcinus orca Ursus maritimus Killer whale Aarhus University: Research Chemosphere 176 243 248 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Mixture toxicology Realistic exposure Freeze-lipid filtration Marine mammal Polychlorinated biphenyls Flame retardants SOLID-PHASE EXTRACTION 3 DECADES 1983-2010 ACTING CHEMICALS VIBRIO-FISCHERI HARBOR SEALS TOXICITY PREDICTABILITY CHROMATOGRAPHY FILTRATION CLEANUP |
spellingShingle |
Mixture toxicology Realistic exposure Freeze-lipid filtration Marine mammal Polychlorinated biphenyls Flame retardants SOLID-PHASE EXTRACTION 3 DECADES 1983-2010 ACTING CHEMICALS VIBRIO-FISCHERI HARBOR SEALS TOXICITY PREDICTABILITY CHROMATOGRAPHY FILTRATION CLEANUP Desforges, Jean-Pierre Eulaers, Igor Periard, Luke Sonne, Christian Dietz, Rune Letcher, Robert J. A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
topic_facet |
Mixture toxicology Realistic exposure Freeze-lipid filtration Marine mammal Polychlorinated biphenyls Flame retardants SOLID-PHASE EXTRACTION 3 DECADES 1983-2010 ACTING CHEMICALS VIBRIO-FISCHERI HARBOR SEALS TOXICITY PREDICTABILITY CHROMATOGRAPHY FILTRATION CLEANUP |
description |
In vitro investigations of the health impact of individual chemical compounds have traditionally been used in risk assessments. However, humans and wildlife are exposed to a plethora of potentially harmful chemicals, including organohalogen contaminants (OHCs). An alternative exposure approach to individual or simple mixtures of synthetic OHCs is to isolate the complex mixture present in free-ranging wildlife, often non-destructively sampled from lipid rich adipose. High concentration stock volumes required for in vitro investigations do, however, pose a great analytical challenge to extract sufficient amounts of complex OHC cocktails. Here we describe a novel method to easily, rapidly and efficiently extract an environmentally accumulated and therefore relevant contaminant cocktail from large (10-50 g) marine mammal blubber samples. We demonstrate that lipid freeze-filtration with acetonitrile removes up to 97% of blubber lipids, with minimal effect on the efficiency of OHC recovery. Sample extracts after freeze-filtration were further processed to remove residual trace lipids via high-pressure gel permeation chromatography and solid phase extraction. Average recoveries of OHCs from triplicate analysis of killer whale (Orcinus orca), polar bear (Ursus maritimus) and pilot whale (Globicephala spp.) blubber standard reference material (NIST SRM-1945) ranged from 68 to 80%, 54-92% and 58-145%, respectively, for C-13-enriched internal standards of six polychlorinated biphenyl congeners, 16 organochlorine pesticides and four brominated flame retardants. This approach to rapidly generate OHC mixtures shows great potential for experimental exposures using complex contaminant mixtures, research or monitoring driven contaminant quantification in biological samples, as well as the untargeted identification of emerging contaminants. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Desforges, Jean-Pierre Eulaers, Igor Periard, Luke Sonne, Christian Dietz, Rune Letcher, Robert J. |
author_facet |
Desforges, Jean-Pierre Eulaers, Igor Periard, Luke Sonne, Christian Dietz, Rune Letcher, Robert J. |
author_sort |
Desforges, Jean-Pierre |
title |
A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
title_short |
A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
title_full |
A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
title_fullStr |
A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
title_full_unstemmed |
A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
title_sort |
rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues |
publishDate |
2017 |
url |
https://pure.au.dk/portal/da/publications/a-rapid-analytical-method-to-quantify-complex-organohalogen-contaminant-mixtures-in-large-samples-of-high-lipid-mammalian-tissues(317f8660-8cf7-4b17-8b71-07652b7900c2).html https://doi.org/10.1016/j.chemosphere.2017.02.098 |
genre |
Killer Whale Orca Orcinus orca Ursus maritimus Killer whale |
genre_facet |
Killer Whale Orca Orcinus orca Ursus maritimus Killer whale |
op_source |
Desforges , J-P , Eulaers , I , Periard , L , Sonne , C , Dietz , R & Letcher , R J 2017 , ' A rapid analytical method to quantify complex organohalogen contaminant mixtures in large samples of high lipid mammalian tissues ' , Chemosphere , vol. 176 , pp. 243-248 . https://doi.org/10.1016/j.chemosphere.2017.02.098 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.chemosphere.2017.02.098 |
container_title |
Chemosphere |
container_volume |
176 |
container_start_page |
243 |
op_container_end_page |
248 |
_version_ |
1766057571478667264 |