Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms
Mannan-binding lectin (MBL) and ficolins distinguish between self, non-self and altered-self by recognizing patterns of ligands on the surface of microorganisms or aberrant cells. When this happens MBL-associated serine protease-2 (MASP-2) is activated and cleaves complement factors to start inflamm...
Published in: | Genes & Immunity |
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2007
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ftuniaarhuspubl:oai:pure.atira.dk:publications/2b735bf3-f138-44ef-bc84-776b18f55ff7 2023-05-15T16:28:21+02:00 Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms Thiel, S Steffensen, R Christensen, I J Ip, W K Lau, Y L Reason, I J M Eiberg, Hans Rudolf Lytchoff Gadjeva, M Ruseva, M Jensenius, Jens Christian 2007 https://pure.au.dk/portal/da/publications/deficiency-of-mannanbinding-lectin-associated-serine-protease2-due-to-missense-polymorphisms(2b735bf3-f138-44ef-bc84-776b18f55ff7).html https://doi.org/10.1038/sj.gene.6364373 eng eng info:eu-repo/semantics/restrictedAccess Thiel , S , Steffensen , R , Christensen , I J , Ip , W K , Lau , Y L , Reason , I J M , Eiberg , H R L , Gadjeva , M , Ruseva , M & Jensenius , J C 2007 , ' Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms ' , Genes and Immunity , vol. 8 , no. 2 , pp. 154-63 . https://doi.org/10.1038/sj.gene.6364373 African Continental Ancestry Group Asian Continental Ancestry Group Brazil DNA Primers Exons Gene Frequency Genotype Greenland Hong Kong Humans Indians South American Inuits Mannose-Binding Protein-Associated Serine Proteases Mutation Missense Polymorphism Genetic Sequence Analysis DNA Zambia article 2007 ftuniaarhuspubl https://doi.org/10.1038/sj.gene.6364373 2023-04-05T22:56:26Z Mannan-binding lectin (MBL) and ficolins distinguish between self, non-self and altered-self by recognizing patterns of ligands on the surface of microorganisms or aberrant cells. When this happens MBL-associated serine protease-2 (MASP-2) is activated and cleaves complement factors to start inflammatory actions. We examined human populations for MASP-2 levels, MASP-2 function and for the presence of mutations in coding exons of MASP2. The MASP-2 levels were lowest in Africans from Zambia (median, 196 ng/ml) followed by Hong Kong Chinese (262 ng/ml), Brazilian Amerindians (290 ng/ml) and Danish Caucasians (416 ng/ml). In the Chinese population, we uncovered a novel four amino-acid tandem duplication (p.156_159dupCHNH) associated with low levels of MASP-2. The frequency of this mutation as well as the SNPs p.R99C, p.R118C, p.D120G, p.P126L and p.V377A were analyzed. The p.156_159dupCHNH was only found in Chinese (gene frequency 0.26%) and p.D120G was found only in Caucasians and Inuits from West-Greenland. The p.P126L and p.R99Q were present in Africans and Amerindians only, except for p.R99Q in one Caucasian. The MASP-2 levels were reduced in individuals with p.V377A present. The MASP-2 present in individuals homozygous for p.377A or p.99Q had a normal enzyme activity whereas MASP-2 in individuals homozygous for p.126L was non-functional. Article in Journal/Newspaper Greenland inuits Aarhus University: Research Greenland Genes & Immunity 8 2 154 163 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
African Continental Ancestry Group Asian Continental Ancestry Group Brazil DNA Primers Exons Gene Frequency Genotype Greenland Hong Kong Humans Indians South American Inuits Mannose-Binding Protein-Associated Serine Proteases Mutation Missense Polymorphism Genetic Sequence Analysis DNA Zambia |
spellingShingle |
African Continental Ancestry Group Asian Continental Ancestry Group Brazil DNA Primers Exons Gene Frequency Genotype Greenland Hong Kong Humans Indians South American Inuits Mannose-Binding Protein-Associated Serine Proteases Mutation Missense Polymorphism Genetic Sequence Analysis DNA Zambia Thiel, S Steffensen, R Christensen, I J Ip, W K Lau, Y L Reason, I J M Eiberg, Hans Rudolf Lytchoff Gadjeva, M Ruseva, M Jensenius, Jens Christian Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
topic_facet |
African Continental Ancestry Group Asian Continental Ancestry Group Brazil DNA Primers Exons Gene Frequency Genotype Greenland Hong Kong Humans Indians South American Inuits Mannose-Binding Protein-Associated Serine Proteases Mutation Missense Polymorphism Genetic Sequence Analysis DNA Zambia |
description |
Mannan-binding lectin (MBL) and ficolins distinguish between self, non-self and altered-self by recognizing patterns of ligands on the surface of microorganisms or aberrant cells. When this happens MBL-associated serine protease-2 (MASP-2) is activated and cleaves complement factors to start inflammatory actions. We examined human populations for MASP-2 levels, MASP-2 function and for the presence of mutations in coding exons of MASP2. The MASP-2 levels were lowest in Africans from Zambia (median, 196 ng/ml) followed by Hong Kong Chinese (262 ng/ml), Brazilian Amerindians (290 ng/ml) and Danish Caucasians (416 ng/ml). In the Chinese population, we uncovered a novel four amino-acid tandem duplication (p.156_159dupCHNH) associated with low levels of MASP-2. The frequency of this mutation as well as the SNPs p.R99C, p.R118C, p.D120G, p.P126L and p.V377A were analyzed. The p.156_159dupCHNH was only found in Chinese (gene frequency 0.26%) and p.D120G was found only in Caucasians and Inuits from West-Greenland. The p.P126L and p.R99Q were present in Africans and Amerindians only, except for p.R99Q in one Caucasian. The MASP-2 levels were reduced in individuals with p.V377A present. The MASP-2 present in individuals homozygous for p.377A or p.99Q had a normal enzyme activity whereas MASP-2 in individuals homozygous for p.126L was non-functional. |
format |
Article in Journal/Newspaper |
author |
Thiel, S Steffensen, R Christensen, I J Ip, W K Lau, Y L Reason, I J M Eiberg, Hans Rudolf Lytchoff Gadjeva, M Ruseva, M Jensenius, Jens Christian |
author_facet |
Thiel, S Steffensen, R Christensen, I J Ip, W K Lau, Y L Reason, I J M Eiberg, Hans Rudolf Lytchoff Gadjeva, M Ruseva, M Jensenius, Jens Christian |
author_sort |
Thiel, S |
title |
Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
title_short |
Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
title_full |
Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
title_fullStr |
Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
title_full_unstemmed |
Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
title_sort |
deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms |
publishDate |
2007 |
url |
https://pure.au.dk/portal/da/publications/deficiency-of-mannanbinding-lectin-associated-serine-protease2-due-to-missense-polymorphisms(2b735bf3-f138-44ef-bc84-776b18f55ff7).html https://doi.org/10.1038/sj.gene.6364373 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland inuits |
genre_facet |
Greenland inuits |
op_source |
Thiel , S , Steffensen , R , Christensen , I J , Ip , W K , Lau , Y L , Reason , I J M , Eiberg , H R L , Gadjeva , M , Ruseva , M & Jensenius , J C 2007 , ' Deficiency of mannan-binding lectin associated serine protease-2 due to missense polymorphisms ' , Genes and Immunity , vol. 8 , no. 2 , pp. 154-63 . https://doi.org/10.1038/sj.gene.6364373 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1038/sj.gene.6364373 |
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Genes & Immunity |
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8 |
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2 |
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154 |
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163 |
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1766017992884224000 |