Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos)
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expr...
Published in: | Clinical and Translational Science |
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2021
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Online Access: | https://pure.au.dk/portal/da/publications/expression-of-paracrine-effectors-in-human-adiposederived-mesenchymal-stem-cells-treated-with-plasma-from-brown-bears-ursus-arctos(26bcb04b-84bf-4fef-8d40-f3be40e4c95d).html https://doi.org/10.1111/cts.12872 http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/26bcb04b-84bf-4fef-8d40-f3be40e4c95d 2023-05-15T18:42:09+02:00 Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole 2021-01 https://pure.au.dk/portal/da/publications/expression-of-paracrine-effectors-in-human-adiposederived-mesenchymal-stem-cells-treated-with-plasma-from-brown-bears-ursus-arctos(26bcb04b-84bf-4fef-8d40-f3be40e4c95d).html https://doi.org/10.1111/cts.12872 http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Berg von Linde , M , Johansson , K , Kruse , R , Helenius , G , Samano , N , Friberg , Ö , Frøbert , A M & Fröbert , O 2021 , ' Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) ' , Clinical and Translational Science , vol. 14 , no. 1 , pp. 317-325 . https://doi.org/10.1111/cts.12872 article 2021 ftuniaarhuspubl https://doi.org/10.1111/cts.12872 2023-03-01T23:56:53Z Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. Article in Journal/Newspaper Ursus arctos Aarhus University: Research Clinical and Translational Science 14 1 317 325 |
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Open Polar |
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Aarhus University: Research |
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ftuniaarhuspubl |
language |
English |
description |
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. |
format |
Article in Journal/Newspaper |
author |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole |
spellingShingle |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
author_facet |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole |
author_sort |
Berg von Linde, Maria |
title |
Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_short |
Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full |
Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_fullStr |
Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full_unstemmed |
Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_sort |
expression of paracrine effectors in human adipose-derived mesenchymal stem cells treated with plasma from brown bears (ursus arctos) |
publishDate |
2021 |
url |
https://pure.au.dk/portal/da/publications/expression-of-paracrine-effectors-in-human-adiposederived-mesenchymal-stem-cells-treated-with-plasma-from-brown-bears-ursus-arctos(26bcb04b-84bf-4fef-8d40-f3be40e4c95d).html https://doi.org/10.1111/cts.12872 http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Berg von Linde , M , Johansson , K , Kruse , R , Helenius , G , Samano , N , Friberg , Ö , Frøbert , A M & Fröbert , O 2021 , ' Expression of Paracrine Effectors in Human Adipose-Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) ' , Clinical and Translational Science , vol. 14 , no. 1 , pp. 317-325 . https://doi.org/10.1111/cts.12872 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/cts.12872 |
container_title |
Clinical and Translational Science |
container_volume |
14 |
container_issue |
1 |
container_start_page |
317 |
op_container_end_page |
325 |
_version_ |
1766231765598339072 |