Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria
Cyanobacteria are thought to be the main N 2 -fixing organisms (diazotrophs) in marine pelagic waters, but recent molecular analyses indicate that non-cyanobacterial diazotrophs are also present and active. Existing data are, however, restricted geographically and by limited sequencing depths. Our a...
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2011
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ftuniaarhuspubl:oai:pure.atira.dk:publications/1ed4684a-079b-4bfe-af1c-2b79f8f7956d 2023-11-12T04:13:36+01:00 Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria Farnelid, Hanna Andersson, Anders F. Bertilsson, Stefan Al-Soud, Waleed Abu Hansen, Lars Hestbjerg Sørensen, Søren Steward, Grieg F. Hagström, Åke Riemann, Lasse 2011 https://pure.au.dk/portal/da/publications/nitrogenase-gene-amplicons-from-global-marine-surface-waters-are-dominated-by-genes-of-noncyanobacteria(1ed4684a-079b-4bfe-af1c-2b79f8f7956d).html https://doi.org/10.1371/journal.pone.0019223 http://www.scopus.com/inward/record.url?scp=79955742866&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/da/publications/nitrogenase-gene-amplicons-from-global-marine-surface-waters-are-dominated-by-genes-of-noncyanobacteria(1ed4684a-079b-4bfe-af1c-2b79f8f7956d).html info:eu-repo/semantics/restrictedAccess Farnelid , H , Andersson , A F , Bertilsson , S , Al-Soud , W A , Hansen , L H , Sørensen , S , Steward , G F , Hagström , Å & Riemann , L 2011 , ' Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria ' , P L o S One , vol. 6 , no. 4 , e19223 . https://doi.org/10.1371/journal.pone.0019223 article 2011 ftuniaarhuspubl https://doi.org/10.1371/journal.pone.0019223 2023-10-25T22:59:02Z Cyanobacteria are thought to be the main N 2 -fixing organisms (diazotrophs) in marine pelagic waters, but recent molecular analyses indicate that non-cyanobacterial diazotrophs are also present and active. Existing data are, however, restricted geographically and by limited sequencing depths. Our analysis of 79,090 nitrogenase (nifH) PCR amplicons encoding 7,468 unique proteins from surface samples (ten DNA samples and two RNA samples) collected at ten marine locations world-wide provides the first in-depth survey of a functional bacterial gene and yield insights into the composition and diversity of the nifH gene pool in marine waters. Great divergence in nifH composition was observed between sites. Cyanobacteria-like genes were most frequent among amplicons from the warmest waters, but overall the data set was dominated by nifH sequences most closely related to non-cyanobacteria. Clusters related to Alpha-, Beta-, Gamma-, and Delta-Proteobacteria were most common and showed distinct geographic distributions. Sequences related to anaerobic bacteria (nifH Cluster III) were generally rare, but preponderant in cold waters, especially in the Arctic. Although the two transcript samples were dominated by unicellular cyanobacteria, 42% of the identified non-cyanobacterial nifH clusters from the corresponding DNA samples were also detected in cDNA. The study indicates that non-cyanobacteria account for a substantial part of the nifH gene pool in marine surface waters and that these genes are at least occasionally expressed. The contribution of non-cyanobacterial diazotrophs to the global N 2 fixation budget cannot be inferred from sequence data alone, but the prevalence of non-cyanobacterial nifH genes and transcripts suggest that these bacteria are ecologically significant. Article in Journal/Newspaper Arctic Aarhus University: Research Arctic PLoS ONE 6 4 e19223 |
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Aarhus University: Research |
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ftuniaarhuspubl |
language |
English |
description |
Cyanobacteria are thought to be the main N 2 -fixing organisms (diazotrophs) in marine pelagic waters, but recent molecular analyses indicate that non-cyanobacterial diazotrophs are also present and active. Existing data are, however, restricted geographically and by limited sequencing depths. Our analysis of 79,090 nitrogenase (nifH) PCR amplicons encoding 7,468 unique proteins from surface samples (ten DNA samples and two RNA samples) collected at ten marine locations world-wide provides the first in-depth survey of a functional bacterial gene and yield insights into the composition and diversity of the nifH gene pool in marine waters. Great divergence in nifH composition was observed between sites. Cyanobacteria-like genes were most frequent among amplicons from the warmest waters, but overall the data set was dominated by nifH sequences most closely related to non-cyanobacteria. Clusters related to Alpha-, Beta-, Gamma-, and Delta-Proteobacteria were most common and showed distinct geographic distributions. Sequences related to anaerobic bacteria (nifH Cluster III) were generally rare, but preponderant in cold waters, especially in the Arctic. Although the two transcript samples were dominated by unicellular cyanobacteria, 42% of the identified non-cyanobacterial nifH clusters from the corresponding DNA samples were also detected in cDNA. The study indicates that non-cyanobacteria account for a substantial part of the nifH gene pool in marine surface waters and that these genes are at least occasionally expressed. The contribution of non-cyanobacterial diazotrophs to the global N 2 fixation budget cannot be inferred from sequence data alone, but the prevalence of non-cyanobacterial nifH genes and transcripts suggest that these bacteria are ecologically significant. |
format |
Article in Journal/Newspaper |
author |
Farnelid, Hanna Andersson, Anders F. Bertilsson, Stefan Al-Soud, Waleed Abu Hansen, Lars Hestbjerg Sørensen, Søren Steward, Grieg F. Hagström, Åke Riemann, Lasse |
spellingShingle |
Farnelid, Hanna Andersson, Anders F. Bertilsson, Stefan Al-Soud, Waleed Abu Hansen, Lars Hestbjerg Sørensen, Søren Steward, Grieg F. Hagström, Åke Riemann, Lasse Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
author_facet |
Farnelid, Hanna Andersson, Anders F. Bertilsson, Stefan Al-Soud, Waleed Abu Hansen, Lars Hestbjerg Sørensen, Søren Steward, Grieg F. Hagström, Åke Riemann, Lasse |
author_sort |
Farnelid, Hanna |
title |
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
title_short |
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
title_full |
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
title_fullStr |
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
title_full_unstemmed |
Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
title_sort |
nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria |
publishDate |
2011 |
url |
https://pure.au.dk/portal/da/publications/nitrogenase-gene-amplicons-from-global-marine-surface-waters-are-dominated-by-genes-of-noncyanobacteria(1ed4684a-079b-4bfe-af1c-2b79f8f7956d).html https://doi.org/10.1371/journal.pone.0019223 http://www.scopus.com/inward/record.url?scp=79955742866&partnerID=8YFLogxK |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Farnelid , H , Andersson , A F , Bertilsson , S , Al-Soud , W A , Hansen , L H , Sørensen , S , Steward , G F , Hagström , Å & Riemann , L 2011 , ' Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria ' , P L o S One , vol. 6 , no. 4 , e19223 . https://doi.org/10.1371/journal.pone.0019223 |
op_relation |
https://pure.au.dk/portal/da/publications/nitrogenase-gene-amplicons-from-global-marine-surface-waters-are-dominated-by-genes-of-noncyanobacteria(1ed4684a-079b-4bfe-af1c-2b79f8f7956d).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0019223 |
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PLoS ONE |
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container_start_page |
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