Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway
Background: Capillary electrophoresis of plasma proteins has shown great potential as a complementary diagnostic tool for avian species. However, reference intervals for plasma proteins are sparse or lacking for several free-living avian species. The current study reports electrophoretic patterns an...
Published in: | BMC Veterinary Research |
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2019
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Online Access: | https://pure.au.dk/portal/da/publications/plasma-protein-fractions-in-freeliving-whitetailed-eagle-haliaeetus-albicilla-nestlings-from-norway(1aac885e-394a-4f88-b0cc-3d355fe6e0c0).html https://doi.org/10.1186/s12917-019-2022-6 http://www.scopus.com/inward/record.url?scp=85069966931&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/1aac885e-394a-4f88-b0cc-3d355fe6e0c0 2023-05-15T16:32:42+02:00 Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway Flo, Jørgen Løseth, Mari Engvig Sonne, Christian Jaspers, Veerle L.B. Brun-Hansen, Hege 2019-08-13 https://pure.au.dk/portal/da/publications/plasma-protein-fractions-in-freeliving-whitetailed-eagle-haliaeetus-albicilla-nestlings-from-norway(1aac885e-394a-4f88-b0cc-3d355fe6e0c0).html https://doi.org/10.1186/s12917-019-2022-6 http://www.scopus.com/inward/record.url?scp=85069966931&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Flo , J , Løseth , M E , Sonne , C , Jaspers , V L B & Brun-Hansen , H 2019 , ' Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway ' , BMC Veterinary Research , vol. 15 , no. 1 , 290 . https://doi.org/10.1186/s12917-019-2022-6 Acute phase protein Albumin Avian diagnostics Capillary electrophoresis Globulin article 2019 ftuniaarhuspubl https://doi.org/10.1186/s12917-019-2022-6 2023-03-08T23:56:41Z Background: Capillary electrophoresis of plasma proteins has shown great potential as a complementary diagnostic tool for avian species. However, reference intervals for plasma proteins are sparse or lacking for several free-living avian species. The current study reports electrophoretic patterns and concentrations of plasma proteins determined for 70 free-living white-tailed eagle (Haliaeetus albicilla) nestlings from two locations in Norway (Steigen and Smøla) in order to establish reference values for this subpopulation using capillary electrophoresis. The nestlings were between 44 and 87 days of age, and the plasma protein concentrations were investigated for age, sex, year (2015 and 2016) and location differences. To our knowledge, this is the first report of reference intervals of plasma proteins analysed by capillary electrophoresis in free-living white-tailed eagle nestlings. Results: The plasma protein concentrations (% of total protein, mean ± SE) were determined for prealbumin (13.7%, 4.34 ± 0.15 g/L), albumin (46.7%, 14.81 ± 0.24 g/L), α 1 -globulin (2.4%, 0.74 ± 0.03 g/L), α 2 -globulin (11.7%, 3.72 ± 0.06 g/L), β-globulin (15.9%, 5.06 ± 0.08 g/L) and γ-globulin (9.6%, 3.05 ± 0.09 g/L). Significant differences were found between the two locations for prealbumin, α 2 - A nd γ-globulins. No significant differences were found between the two sampling years or sexes, and no effect of age was found for any of the plasma proteins. However, prealbumin levels were several folds higher than previously reported from adults of closely related birds of prey species. There were no other studies on capillary electrophoresis of nestling plasma available for comparison. Conclusion: Significant differences were found between sampling locations for prealbumin, α 2 - A nd γ-globulins, which may indicate differences in inflammatory or infectious status between nestlings at the two locations. Sampling year, sex or age had no significant effect on the plasma protein concentrations. These results provide novel data on ... Article in Journal/Newspaper Haliaeetus albicilla White-tailed eagle Aarhus University: Research Norway Smøla ENVELOPE(8.034,8.034,63.357,63.357) BMC Veterinary Research 15 1 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Acute phase protein Albumin Avian diagnostics Capillary electrophoresis Globulin |
spellingShingle |
Acute phase protein Albumin Avian diagnostics Capillary electrophoresis Globulin Flo, Jørgen Løseth, Mari Engvig Sonne, Christian Jaspers, Veerle L.B. Brun-Hansen, Hege Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
topic_facet |
Acute phase protein Albumin Avian diagnostics Capillary electrophoresis Globulin |
description |
Background: Capillary electrophoresis of plasma proteins has shown great potential as a complementary diagnostic tool for avian species. However, reference intervals for plasma proteins are sparse or lacking for several free-living avian species. The current study reports electrophoretic patterns and concentrations of plasma proteins determined for 70 free-living white-tailed eagle (Haliaeetus albicilla) nestlings from two locations in Norway (Steigen and Smøla) in order to establish reference values for this subpopulation using capillary electrophoresis. The nestlings were between 44 and 87 days of age, and the plasma protein concentrations were investigated for age, sex, year (2015 and 2016) and location differences. To our knowledge, this is the first report of reference intervals of plasma proteins analysed by capillary electrophoresis in free-living white-tailed eagle nestlings. Results: The plasma protein concentrations (% of total protein, mean ± SE) were determined for prealbumin (13.7%, 4.34 ± 0.15 g/L), albumin (46.7%, 14.81 ± 0.24 g/L), α 1 -globulin (2.4%, 0.74 ± 0.03 g/L), α 2 -globulin (11.7%, 3.72 ± 0.06 g/L), β-globulin (15.9%, 5.06 ± 0.08 g/L) and γ-globulin (9.6%, 3.05 ± 0.09 g/L). Significant differences were found between the two locations for prealbumin, α 2 - A nd γ-globulins. No significant differences were found between the two sampling years or sexes, and no effect of age was found for any of the plasma proteins. However, prealbumin levels were several folds higher than previously reported from adults of closely related birds of prey species. There were no other studies on capillary electrophoresis of nestling plasma available for comparison. Conclusion: Significant differences were found between sampling locations for prealbumin, α 2 - A nd γ-globulins, which may indicate differences in inflammatory or infectious status between nestlings at the two locations. Sampling year, sex or age had no significant effect on the plasma protein concentrations. These results provide novel data on ... |
format |
Article in Journal/Newspaper |
author |
Flo, Jørgen Løseth, Mari Engvig Sonne, Christian Jaspers, Veerle L.B. Brun-Hansen, Hege |
author_facet |
Flo, Jørgen Løseth, Mari Engvig Sonne, Christian Jaspers, Veerle L.B. Brun-Hansen, Hege |
author_sort |
Flo, Jørgen |
title |
Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
title_short |
Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
title_full |
Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
title_fullStr |
Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
title_full_unstemmed |
Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway |
title_sort |
plasma protein fractions in free-living white-tailed eagle (haliaeetus albicilla) nestlings from norway |
publishDate |
2019 |
url |
https://pure.au.dk/portal/da/publications/plasma-protein-fractions-in-freeliving-whitetailed-eagle-haliaeetus-albicilla-nestlings-from-norway(1aac885e-394a-4f88-b0cc-3d355fe6e0c0).html https://doi.org/10.1186/s12917-019-2022-6 http://www.scopus.com/inward/record.url?scp=85069966931&partnerID=8YFLogxK |
long_lat |
ENVELOPE(8.034,8.034,63.357,63.357) |
geographic |
Norway Smøla |
geographic_facet |
Norway Smøla |
genre |
Haliaeetus albicilla White-tailed eagle |
genre_facet |
Haliaeetus albicilla White-tailed eagle |
op_source |
Flo , J , Løseth , M E , Sonne , C , Jaspers , V L B & Brun-Hansen , H 2019 , ' Plasma protein fractions in free-living white-tailed eagle (Haliaeetus albicilla) nestlings from Norway ' , BMC Veterinary Research , vol. 15 , no. 1 , 290 . https://doi.org/10.1186/s12917-019-2022-6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12917-019-2022-6 |
container_title |
BMC Veterinary Research |
container_volume |
15 |
container_issue |
1 |
_version_ |
1766022453703737344 |