Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil
Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanugin...
Published in: | Bulletin of Chemical Reaction Engineering & Catalysis |
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Department of Chemical Engineering - Diponegoro University
2020
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Online Access: | https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648 https://doi.org/10.9767/bcrec.15.1.6648.242-252 |
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ftundipojs2:oai:ojs.ejournal2.undip.ac.id:article/6648 2023-05-15T13:55:12+02:00 Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil Gusniah, Azianna Veny, Harumi Hamzah, Fazlena Universiti Teknologi MARA (UiTM) for financial and technically supporting this research under Dana/KCM 5/3/Lestari (132/2017) and 600-IRMI/DANA 5/3/REI (0009/2016) 2020-04-01 application/pdf https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648 https://doi.org/10.9767/bcrec.15.1.6648.242-252 eng eng Department of Chemical Engineering - Diponegoro University https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648/3655 https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648 doi:10.9767/bcrec.15.1.6648.242-252 Copyright (c) 2020 by Authors, Published by BCREC Group http://creativecommons.org/licenses/by-sa/4.0 CC-BY-SA Bulletin of Chemical Reaction Engineering & Catalysis; 2020: BCREC Volume 15 Issue 1 Year 2020 (April 2020); 242-252 1978-2993 Enzyme Activity Immobilized Lipase Transesterifications Waste Cooking Oil info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2020 ftundipojs2 https://doi.org/10.9767/bcrec.15.1.6648.242-252 2022-08-07T06:25:40Z Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanuginosus, TLIM, and Candida Antarctica A (recombinant from Aspergillus oryzae), CALA. These lipases were compared based on their activity at different pH (6-10), temperature (30-50 °C), activation energy, and amount of lipase loading for hydrolysis of p-NPA into n-NP. The result indicates that among the lipase used in the study, CALA is the preferable biocatalyst in the hydrolysis of p-NPA due to the minimum energy required and higher enzymatic activity at 20 mg of enzyme loading. PcL and CALA used in the study gave the optimum activity at pH 9 except for TLIM at pH 8 and the optimum temperature at 40 °C. The kinetic data obtained for CALA in this reaction were Km = 57.412 mM and Vm = 70 µM/min. This finding shows that CALA is beneficial biocatalysts for the transesterification process to obtain a higher product with lower activation energy. Copyright © 2020 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). Article in Journal/Newspaper Antarc* Antarctica Universitas Diponegoro: Undip E-Journal System (UEJS) Portal Bulletin of Chemical Reaction Engineering & Catalysis 15 1 242 252 |
institution |
Open Polar |
collection |
Universitas Diponegoro: Undip E-Journal System (UEJS) Portal |
op_collection_id |
ftundipojs2 |
language |
English |
topic |
Enzyme Activity Immobilized Lipase Transesterifications Waste Cooking Oil |
spellingShingle |
Enzyme Activity Immobilized Lipase Transesterifications Waste Cooking Oil Gusniah, Azianna Veny, Harumi Hamzah, Fazlena Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
topic_facet |
Enzyme Activity Immobilized Lipase Transesterifications Waste Cooking Oil |
description |
Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanuginosus, TLIM, and Candida Antarctica A (recombinant from Aspergillus oryzae), CALA. These lipases were compared based on their activity at different pH (6-10), temperature (30-50 °C), activation energy, and amount of lipase loading for hydrolysis of p-NPA into n-NP. The result indicates that among the lipase used in the study, CALA is the preferable biocatalyst in the hydrolysis of p-NPA due to the minimum energy required and higher enzymatic activity at 20 mg of enzyme loading. PcL and CALA used in the study gave the optimum activity at pH 9 except for TLIM at pH 8 and the optimum temperature at 40 °C. The kinetic data obtained for CALA in this reaction were Km = 57.412 mM and Vm = 70 µM/min. This finding shows that CALA is beneficial biocatalysts for the transesterification process to obtain a higher product with lower activation energy. Copyright © 2020 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). |
author2 |
Universiti Teknologi MARA (UiTM) for financial and technically supporting this research under Dana/KCM 5/3/Lestari (132/2017) and 600-IRMI/DANA 5/3/REI (0009/2016) |
format |
Article in Journal/Newspaper |
author |
Gusniah, Azianna Veny, Harumi Hamzah, Fazlena |
author_facet |
Gusniah, Azianna Veny, Harumi Hamzah, Fazlena |
author_sort |
Gusniah, Azianna |
title |
Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
title_short |
Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
title_full |
Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
title_fullStr |
Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
title_full_unstemmed |
Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil |
title_sort |
activity and stability of immobilized lipase for utilization in transesterification of waste cooking oil |
publisher |
Department of Chemical Engineering - Diponegoro University |
publishDate |
2020 |
url |
https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648 https://doi.org/10.9767/bcrec.15.1.6648.242-252 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Bulletin of Chemical Reaction Engineering & Catalysis; 2020: BCREC Volume 15 Issue 1 Year 2020 (April 2020); 242-252 1978-2993 |
op_relation |
https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648/3655 https://ejournal2.undip.ac.id/index.php/bcrec/article/view/6648 doi:10.9767/bcrec.15.1.6648.242-252 |
op_rights |
Copyright (c) 2020 by Authors, Published by BCREC Group http://creativecommons.org/licenses/by-sa/4.0 |
op_rightsnorm |
CC-BY-SA |
op_doi |
https://doi.org/10.9767/bcrec.15.1.6648.242-252 |
container_title |
Bulletin of Chemical Reaction Engineering & Catalysis |
container_volume |
15 |
container_issue |
1 |
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242 |
op_container_end_page |
252 |
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1766261486368325632 |