Heat shock and cold adaptation in antarctic fishes: a molecular approach
1. 1. The restriction patterns of three different Antarctic fish DNA (P. bernacchi, N. rossi and C. kathleene) have been analysed. 2. 2. The restricted DNAs have been probed for the presence of the heat shock 70 sequence (hsp70) by using the corresponding Drosophila sequence. The three patterns are...
Published in: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry |
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1988
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Online Access: | http://hdl.handle.net/11591/188957 https://doi.org/10.1016/0305-0491(88)90303-3 |
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ftuncampaniairis:oai:iris.unicampania.it:11591/188957 2024-04-14T08:02:32+00:00 Heat shock and cold adaptation in antarctic fishes: a molecular approach MARESCA B PATRIARCA E GOLDENBERG C SACCO, Margherita Maresca, B Patriarca, E Goldenberg, C Sacco, Margherita 1988 http://hdl.handle.net/11591/188957 https://doi.org/10.1016/0305-0491(88)90303-3 eng eng volume:90 issue:3 firstpage:623 lastpage:629 numberofpages:7 journal:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY http://hdl.handle.net/11591/188957 doi:10.1016/0305-0491(88)90303-3 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0000486169 info:eu-repo/semantics/article 1988 ftuncampaniairis https://doi.org/10.1016/0305-0491(88)90303-3 2024-03-21T16:00:18Z 1. 1. The restriction patterns of three different Antarctic fish DNA (P. bernacchi, N. rossi and C. kathleene) have been analysed. 2. 2. The restricted DNAs have been probed for the presence of the heat shock 70 sequence (hsp70) by using the corresponding Drosophila sequence. The three patterns are closely related for the presence of several bands in common, though a closer arrangement for the hsp70 gene is present in P. bernacchi and N. rossi. The analysis with different stringency conditions has also suggested that in these fishes more than one copy of hsp70 per genome is present. 3. 3. Poly-A RNA analysis with Northern blot have shown that the hsp70 gene is transcribed, upon temperature increase, between 5 and 12°C with a maximum of expression at 8°C. © 1988. Article in Journal/Newspaper Antarc* Antarctic Università degli Studi della Campania "Luigi Vanvitelli": CINECA IRIS V: Antarctic Bernacchi ENVELOPE(163.850,163.850,-77.483,-77.483) Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 90 3 623 629 |
institution |
Open Polar |
collection |
Università degli Studi della Campania "Luigi Vanvitelli": CINECA IRIS V: |
op_collection_id |
ftuncampaniairis |
language |
English |
description |
1. 1. The restriction patterns of three different Antarctic fish DNA (P. bernacchi, N. rossi and C. kathleene) have been analysed. 2. 2. The restricted DNAs have been probed for the presence of the heat shock 70 sequence (hsp70) by using the corresponding Drosophila sequence. The three patterns are closely related for the presence of several bands in common, though a closer arrangement for the hsp70 gene is present in P. bernacchi and N. rossi. The analysis with different stringency conditions has also suggested that in these fishes more than one copy of hsp70 per genome is present. 3. 3. Poly-A RNA analysis with Northern blot have shown that the hsp70 gene is transcribed, upon temperature increase, between 5 and 12°C with a maximum of expression at 8°C. © 1988. |
author2 |
Maresca, B Patriarca, E Goldenberg, C Sacco, Margherita |
format |
Article in Journal/Newspaper |
author |
MARESCA B PATRIARCA E GOLDENBERG C SACCO, Margherita |
spellingShingle |
MARESCA B PATRIARCA E GOLDENBERG C SACCO, Margherita Heat shock and cold adaptation in antarctic fishes: a molecular approach |
author_facet |
MARESCA B PATRIARCA E GOLDENBERG C SACCO, Margherita |
author_sort |
MARESCA B |
title |
Heat shock and cold adaptation in antarctic fishes: a molecular approach |
title_short |
Heat shock and cold adaptation in antarctic fishes: a molecular approach |
title_full |
Heat shock and cold adaptation in antarctic fishes: a molecular approach |
title_fullStr |
Heat shock and cold adaptation in antarctic fishes: a molecular approach |
title_full_unstemmed |
Heat shock and cold adaptation in antarctic fishes: a molecular approach |
title_sort |
heat shock and cold adaptation in antarctic fishes: a molecular approach |
publishDate |
1988 |
url |
http://hdl.handle.net/11591/188957 https://doi.org/10.1016/0305-0491(88)90303-3 |
long_lat |
ENVELOPE(163.850,163.850,-77.483,-77.483) |
geographic |
Antarctic Bernacchi |
geographic_facet |
Antarctic Bernacchi |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
volume:90 issue:3 firstpage:623 lastpage:629 numberofpages:7 journal:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY http://hdl.handle.net/11591/188957 doi:10.1016/0305-0491(88)90303-3 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0000486169 |
op_doi |
https://doi.org/10.1016/0305-0491(88)90303-3 |
container_title |
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry |
container_volume |
90 |
container_issue |
3 |
container_start_page |
623 |
op_container_end_page |
629 |
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1796315614687526912 |