Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii
Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified f...
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/471076 2024-04-14T08:04:11+00:00 Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina 2022 https://hdl.handle.net/11581/471076 https://doi.org/10.3390/microorganisms10122415 https://www.mdpi.com/2076-2607/10/12/2415 eng eng info:eu-repo/semantics/altIdentifier/pmid/36557668 info:eu-repo/semantics/altIdentifier/wos/WOS:000903723800001 volume:10 issue:12 firstpage:1 lastpage:13 numberofpages:13 journal:MICROORGANISMS https://hdl.handle.net/11581/471076 doi:10.3390/microorganisms10122415 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85144668925 https://www.mdpi.com/2076-2607/10/12/2415 info:eu-repo/semantics/openAccess info:eu-repo/semantics/article 2022 ftuncamerinoiris https://doi.org/10.3390/microorganisms10122415 2024-03-21T20:36:26Z Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified five beta-tubulin isotypes by genome sequencing, named EFBTU1, EFBTU2, EFBTU3, EFBTU4 and EFBTU5. By using polyclonal antibodies directed against EFBTU2/EFBTU1 and EFBTU3, we show that the former isotypes appear to be involved in the formation of all microtubular structures and are particularly abundant in cilia, whereas the latter specifically localizes at the bases of cilia. By RNA interference (RNAi) technology, we silenced the EFBTU3 gene and provided evidence that this isotype has a relevant role in cilia regeneration upon deciliation and in cell division. These results support the long-standing concept that tubulin isotypes possess functional specificity in building diverse microtubular structures. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic Microorganisms 10 12 2415 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular structures in a single cell throughout the life cycle, a feature that made them useful models to study microtubule complexity and the role of tubulin isotypes. In the Antarctic ciliate Euplotes focardii we identified five beta-tubulin isotypes by genome sequencing, named EFBTU1, EFBTU2, EFBTU3, EFBTU4 and EFBTU5. By using polyclonal antibodies directed against EFBTU2/EFBTU1 and EFBTU3, we show that the former isotypes appear to be involved in the formation of all microtubular structures and are particularly abundant in cilia, whereas the latter specifically localizes at the bases of cilia. By RNA interference (RNAi) technology, we silenced the EFBTU3 gene and provided evidence that this isotype has a relevant role in cilia regeneration upon deciliation and in cell division. These results support the long-standing concept that tubulin isotypes possess functional specificity in building diverse microtubular structures. |
author2 |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
format |
Article in Journal/Newspaper |
author |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
spellingShingle |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
author_facet |
Pucciarelli, Sandra Sparvoli, Daniela Ballarini, Patrizia Piersanti, Angela Mozzicafreddo, Matteo Arregui, Lucia Miceli, Cristina |
author_sort |
Pucciarelli, Sandra |
title |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_short |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_full |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_fullStr |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_full_unstemmed |
Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii |
title_sort |
ciliate microtubule diversities: insights from the efbtu3 tubulin in the antarctic ciliate euplotes focardii |
publishDate |
2022 |
url |
https://hdl.handle.net/11581/471076 https://doi.org/10.3390/microorganisms10122415 https://www.mdpi.com/2076-2607/10/12/2415 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/36557668 info:eu-repo/semantics/altIdentifier/wos/WOS:000903723800001 volume:10 issue:12 firstpage:1 lastpage:13 numberofpages:13 journal:MICROORGANISMS https://hdl.handle.net/11581/471076 doi:10.3390/microorganisms10122415 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85144668925 https://www.mdpi.com/2076-2607/10/12/2415 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/microorganisms10122415 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2415 |
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1796300600639488000 |