Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable str...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2021
|
Subjects: | |
Online Access: | http://hdl.handle.net/11581/452972 https://doi.org/10.3390/md19050263 https://www.mdpi.com/1660-3397/19/5/263 |
id |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/452972 |
---|---|
record_format |
openpolar |
spelling |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/452972 2024-04-14T08:03:53+00:00 Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents John M. S. Nagoth J. A. Zannotti M. Giovannetti R. Mancini A. Ramasamy K. P. Miceli C. Pucciarelli S. John, M. S. Nagoth, J. A. Zannotti, M. Giovannetti, R. Mancini, A. Ramasamy, K. P. Miceli, C. Pucciarelli, S. 2021 http://hdl.handle.net/11581/452972 https://doi.org/10.3390/md19050263 https://www.mdpi.com/1660-3397/19/5/263 eng eng info:eu-repo/semantics/altIdentifier/pmid/34066868 info:eu-repo/semantics/altIdentifier/wos/WOS:000654431300001 volume:19 issue:5 firstpage:1 lastpage:13 numberofpages:13 journal:MARINE DRUGS http://hdl.handle.net/11581/452972 doi:10.3390/md19050263 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85106495613 https://www.mdpi.com/1660-3397/19/5/263 info:eu-repo/semantics/openAccess info:eu-repo/semantics/article 2021 ftuncamerinoiris https://doi.org/10.3390/md19050263 2024-03-21T20:38:37Z In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic Marine Drugs 19 5 263 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. |
author2 |
John, M. S. Nagoth, J. A. Zannotti, M. Giovannetti, R. Mancini, A. Ramasamy, K. P. Miceli, C. Pucciarelli, S. |
format |
Article in Journal/Newspaper |
author |
John M. S. Nagoth J. A. Zannotti M. Giovannetti R. Mancini A. Ramasamy K. P. Miceli C. Pucciarelli S. |
spellingShingle |
John M. S. Nagoth J. A. Zannotti M. Giovannetti R. Mancini A. Ramasamy K. P. Miceli C. Pucciarelli S. Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
author_facet |
John M. S. Nagoth J. A. Zannotti M. Giovannetti R. Mancini A. Ramasamy K. P. Miceli C. Pucciarelli S. |
author_sort |
John M. S. |
title |
Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
title_short |
Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
title_full |
Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
title_fullStr |
Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
title_full_unstemmed |
Biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: Characterization and potential application as antimicrobial agents |
title_sort |
biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: characterization and potential application as antimicrobial agents |
publishDate |
2021 |
url |
http://hdl.handle.net/11581/452972 https://doi.org/10.3390/md19050263 https://www.mdpi.com/1660-3397/19/5/263 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/34066868 info:eu-repo/semantics/altIdentifier/wos/WOS:000654431300001 volume:19 issue:5 firstpage:1 lastpage:13 numberofpages:13 journal:MARINE DRUGS http://hdl.handle.net/11581/452972 doi:10.3390/md19050263 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85106495613 https://www.mdpi.com/1660-3397/19/5/263 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/md19050263 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
5 |
container_start_page |
263 |
_version_ |
1796300200244936704 |