Bioinformatics Challenges and Potentialities in Studying Extreme Environments
Cold environments are populated by organisms able to contravene deleterious effects of low temperature by diverse adaptive strategies, including the production of ice binding proteins (IBPs) that inhibit the growth of ice crystals inside and outside cells. We describe the properties of such a protei...
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/397804 2024-04-14T08:03:53+00:00 Bioinformatics Challenges and Potentialities in Studying Extreme Environments Angione, Claudio Liò, Pietro PUCCIARELLI, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea Claudio Angione, Pietro Liò, Sandra Pucciarelli, Basarbatu Can, Maxwell Conway, Marina Lotti, Habib Bokhari, Alessio Mancini, Ugur Sezerman, Andrea Telatin Claudia Angelini, Paola MV Rancoita, Stefano Rovetta Angione, Claudio Liò, Pietro Pucciarelli, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea 2016 STAMPA http://hdl.handle.net/11581/397804 https://doi.org/10.1007/978-3-319-44332-4_16 https://link.springer.com/chapter/10.1007/978-3-319-44332-4_16 eng eng Springer country:DEU place:Cham info:eu-repo/semantics/altIdentifier/isbn/978-3-319-44331-7 ispartofbook:Computational Intelligence Methods for Bioinformatics and Biostatistics. CIBB 2015. Lecture Notes in Computer Science, vol 9874 volume:9874 firstpage:205 lastpage:219 numberofpages:15 info:eu-repo/grantAgreement/EC/H2020/MSCA-RISE metable 645693 alleditors:Claudia Angelini, Paola MV Rancoita, Stefano Rovetta http://hdl.handle.net/11581/397804 doi:10.1007/978-3-319-44332-4_16 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84981263663 https://link.springer.com/chapter/10.1007/978-3-319-44332-4_16 info:eu-repo/semantics/closedAccess info:eu-repo/semantics/bookPart 2016 ftuncamerinoiris https://doi.org/10.1007/978-3-319-44332-4_16 2024-03-21T20:37:40Z Cold environments are populated by organisms able to contravene deleterious effects of low temperature by diverse adaptive strategies, including the production of ice binding proteins (IBPs) that inhibit the growth of ice crystals inside and outside cells. We describe the properties of such a protein (EfcIBP) identified in the metagenome of an Antarctic biological consortium composed of the ciliate Euplotes focardii and psychrophilic non-cultured bacteria. Recombinant EfcIBP can resist freezing without any conformational damage and is moderately heat stable, with a midpoint temperature of 66.4 degrees C. Tested for its effects on ice, EfcIBP shows an unusual combination of properties not reported in other bacterial IBPs. First, it is one of the best-performing IBPs described to date in the inhibition of ice recrystallization, with effective concentrations in the nanomolar range. Moreover, EfcIBP has thermal hysteresis activity (0.53 degrees C at 50 mu M) and it can stop a crystal from growing when held at a constant temperature within the thermal hysteresis gap. EfcIBP protects purified proteins and bacterial cells from freezing damage when exposed to challenging temperatures. EfcIBP also possesses a potential N-terminal signal sequence for protein transport and a DUF3494 domain that is common to secreted IBPs. These features lead us to hypothesize that the protein is either anchored at the outer cell surface or concentrated around cells to provide survival advantage to the whole cell consortium. Book Part Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic 205 219 |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
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ftuncamerinoiris |
language |
English |
description |
Cold environments are populated by organisms able to contravene deleterious effects of low temperature by diverse adaptive strategies, including the production of ice binding proteins (IBPs) that inhibit the growth of ice crystals inside and outside cells. We describe the properties of such a protein (EfcIBP) identified in the metagenome of an Antarctic biological consortium composed of the ciliate Euplotes focardii and psychrophilic non-cultured bacteria. Recombinant EfcIBP can resist freezing without any conformational damage and is moderately heat stable, with a midpoint temperature of 66.4 degrees C. Tested for its effects on ice, EfcIBP shows an unusual combination of properties not reported in other bacterial IBPs. First, it is one of the best-performing IBPs described to date in the inhibition of ice recrystallization, with effective concentrations in the nanomolar range. Moreover, EfcIBP has thermal hysteresis activity (0.53 degrees C at 50 mu M) and it can stop a crystal from growing when held at a constant temperature within the thermal hysteresis gap. EfcIBP protects purified proteins and bacterial cells from freezing damage when exposed to challenging temperatures. EfcIBP also possesses a potential N-terminal signal sequence for protein transport and a DUF3494 domain that is common to secreted IBPs. These features lead us to hypothesize that the protein is either anchored at the outer cell surface or concentrated around cells to provide survival advantage to the whole cell consortium. |
author2 |
Claudio Angione, Pietro Liò, Sandra Pucciarelli, Basarbatu Can, Maxwell Conway, Marina Lotti, Habib Bokhari, Alessio Mancini, Ugur Sezerman, Andrea Telatin Claudia Angelini, Paola MV Rancoita, Stefano Rovetta Angione, Claudio Liò, Pietro Pucciarelli, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea |
format |
Book Part |
author |
Angione, Claudio Liò, Pietro PUCCIARELLI, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea |
spellingShingle |
Angione, Claudio Liò, Pietro PUCCIARELLI, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
author_facet |
Angione, Claudio Liò, Pietro PUCCIARELLI, Sandra Can, Basarbatu Conway, Maxwell Lotti, Marina Bokhari, Habib Mancini, Alessio Sezerman, Ugur Telatin, Andrea |
author_sort |
Angione, Claudio |
title |
Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
title_short |
Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
title_full |
Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
title_fullStr |
Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
title_full_unstemmed |
Bioinformatics Challenges and Potentialities in Studying Extreme Environments |
title_sort |
bioinformatics challenges and potentialities in studying extreme environments |
publisher |
Springer |
publishDate |
2016 |
url |
http://hdl.handle.net/11581/397804 https://doi.org/10.1007/978-3-319-44332-4_16 https://link.springer.com/chapter/10.1007/978-3-319-44332-4_16 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/978-3-319-44331-7 ispartofbook:Computational Intelligence Methods for Bioinformatics and Biostatistics. CIBB 2015. Lecture Notes in Computer Science, vol 9874 volume:9874 firstpage:205 lastpage:219 numberofpages:15 info:eu-repo/grantAgreement/EC/H2020/MSCA-RISE metable 645693 alleditors:Claudia Angelini, Paola MV Rancoita, Stefano Rovetta http://hdl.handle.net/11581/397804 doi:10.1007/978-3-319-44332-4_16 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84981263663 https://link.springer.com/chapter/10.1007/978-3-319-44332-4_16 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/978-3-319-44332-4_16 |
container_start_page |
205 |
op_container_end_page |
219 |
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1796300194889859072 |