Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi
Among the species of Euplotes that we are studying for the biology of their water-borne signaling protein pheromones, we have recently focused particular interest on E. petzi for two major reasons. It is a cold-loving (psychrophilic) species dwelling in the freezing Antarctic and Arctic sea waters,...
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/391686 2024-01-28T10:01:30+01:00 Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi VALLESI, Adriana ALIMENTI, Claudio LUPORINI, Pierangelo B. Pedrini Vallesi, Adriana Alimenti, Claudio Luporini, Pierangelo B., Pedrini 2015 STAMPA http://hdl.handle.net/11581/391686 https://isas.unicam.it/2015-ciliate-molecular-biology-conference eng eng Università di Camerino country:ITA place:Camerino ispartofbook:Ciliate Molecular Biology 2015 Book of abstracts 2015 Ciliate Molecular Biology Conference firstpage:56 lastpage:56 numberofpages:1 http://hdl.handle.net/11581/391686 https://isas.unicam.it/2015-ciliate-molecular-biology-conference info:eu-repo/semantics/openAccess info:eu-repo/semantics/conferenceObject 2015 ftuncamerinoiris 2024-01-03T17:43:30Z Among the species of Euplotes that we are studying for the biology of their water-borne signaling protein pheromones, we have recently focused particular interest on E. petzi for two major reasons. It is a cold-loving (psychrophilic) species dwelling in the freezing Antarctic and Arctic sea waters, and forms, together with E. sinicus, the earliest branch of the Euplotes phylogentic tree. Using cultures of genetically distinct E. petzi strains, we have isolated and structurally characterized a family of four pheromones. With respect to the pheromone families known from E. raikovi, E. octocarinatus, E. nobilii and E. crassus, the E. petzi pheromones have the smallest dimensions of only 32 amino acids and the highest density of Cys residues lying in strictly conserved positions and predicted to form four intra-chain disulfide bridges. Although this high density of disulfide bridges would intuitively imply a quite compact globular molecular structure of these molecules, the nuclear magnetic resonance (NMR) determination of the solution structures of the two E. petzi pheromones designated “Ep-1” and “Ep-2” challenges this rationale. This determination shows that the molecular fold of E. petzi pheromones is characterized by a single extended alpha-helix, and by a spatial predominance of regions devoid of regular secondary organization. Considering that a three-helix fold and comparatively reduced extensions of un-structured regions are distinctive traits of the pheromone structures of Euplotes species which live in temperate waters and branch later than E. petzi in the Euplotes phylogenetic tree, our findings suggest two conclusions. The first one, of phylogenetic nature, is that the Euplotes pheromone structure evolves by increasing in dimensions and complexity. It appears to be further supported by the finding that also the macronuclear E. petzi pheromone genes have markedly reduced dimensions and a much simpler organization than their omologs of other Euplotes species. The second one, of adaptive nature, is that ... Conference Object Antarc* Antarctic Arctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Arctic Antarctic |
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Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
Among the species of Euplotes that we are studying for the biology of their water-borne signaling protein pheromones, we have recently focused particular interest on E. petzi for two major reasons. It is a cold-loving (psychrophilic) species dwelling in the freezing Antarctic and Arctic sea waters, and forms, together with E. sinicus, the earliest branch of the Euplotes phylogentic tree. Using cultures of genetically distinct E. petzi strains, we have isolated and structurally characterized a family of four pheromones. With respect to the pheromone families known from E. raikovi, E. octocarinatus, E. nobilii and E. crassus, the E. petzi pheromones have the smallest dimensions of only 32 amino acids and the highest density of Cys residues lying in strictly conserved positions and predicted to form four intra-chain disulfide bridges. Although this high density of disulfide bridges would intuitively imply a quite compact globular molecular structure of these molecules, the nuclear magnetic resonance (NMR) determination of the solution structures of the two E. petzi pheromones designated “Ep-1” and “Ep-2” challenges this rationale. This determination shows that the molecular fold of E. petzi pheromones is characterized by a single extended alpha-helix, and by a spatial predominance of regions devoid of regular secondary organization. Considering that a three-helix fold and comparatively reduced extensions of un-structured regions are distinctive traits of the pheromone structures of Euplotes species which live in temperate waters and branch later than E. petzi in the Euplotes phylogenetic tree, our findings suggest two conclusions. The first one, of phylogenetic nature, is that the Euplotes pheromone structure evolves by increasing in dimensions and complexity. It appears to be further supported by the finding that also the macronuclear E. petzi pheromone genes have markedly reduced dimensions and a much simpler organization than their omologs of other Euplotes species. The second one, of adaptive nature, is that ... |
author2 |
Vallesi, Adriana Alimenti, Claudio Luporini, Pierangelo B., Pedrini |
format |
Conference Object |
author |
VALLESI, Adriana ALIMENTI, Claudio LUPORINI, Pierangelo B. Pedrini |
spellingShingle |
VALLESI, Adriana ALIMENTI, Claudio LUPORINI, Pierangelo B. Pedrini Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
author_facet |
VALLESI, Adriana ALIMENTI, Claudio LUPORINI, Pierangelo B. Pedrini |
author_sort |
VALLESI, Adriana |
title |
Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
title_short |
Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
title_full |
Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
title_fullStr |
Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
title_full_unstemmed |
Structural characterization of the protein pheromones from the psychrophilic and early branching Euplotes species, E. petzi |
title_sort |
structural characterization of the protein pheromones from the psychrophilic and early branching euplotes species, e. petzi |
publisher |
Università di Camerino |
publishDate |
2015 |
url |
http://hdl.handle.net/11581/391686 https://isas.unicam.it/2015-ciliate-molecular-biology-conference |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
ispartofbook:Ciliate Molecular Biology 2015 Book of abstracts 2015 Ciliate Molecular Biology Conference firstpage:56 lastpage:56 numberofpages:1 http://hdl.handle.net/11581/391686 https://isas.unicam.it/2015-ciliate-molecular-biology-conference |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1789326541873741824 |