THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES
Euplotes species are valuable for the study of the structural and functional biology of water-borne protein pheromones that cells constitutively synthesize and use in intra-specific chemical communication. We have recently devoted particular attention to the pheromone family of the “cold loving” (ps...
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Universidad de Sevilla
2015
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/391681 2024-01-28T10:00:43+01:00 THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES VALLESI, Adriana ALIMENTI, Claudio Bill Pedrini LUPORINI, Pierangelo Vallesi, Adriana Alimenti, Claudio Bill, Pedrini Luporini, Pierangelo 2015 STAMPA http://hdl.handle.net/11581/391681 http://feps.ciccartuja.es/ecop/ eng eng Universidad de Sevilla country:ESP place:Sevilla ispartofbook:VII European Congress of Protistology in partnership with The International Society of Protistologists Final programme abstracts VII European Congress of Protistology firstpage:400 lastpage:400 numberofpages:1 http://hdl.handle.net/11581/391681 http://feps.ciccartuja.es/ecop/ info:eu-repo/semantics/openAccess info:eu-repo/semantics/conferenceObject 2015 ftuncamerinoiris 2024-01-03T17:43:29Z Euplotes species are valuable for the study of the structural and functional biology of water-borne protein pheromones that cells constitutively synthesize and use in intra-specific chemical communication. We have recently devoted particular attention to the pheromone family of the “cold loving” (psychrophilic) species E. petzi which dwells in the freezing Antarctic and Arctic coastal sea waters, and forms, together with E. sinicus, the earliest branch of the Euplotes phylogenetic tree. From cultures of genetically distinct strains, we have isolated and sequenced four E. petzi pheromones. With respect to the known pheromones from E. raikovi, E. octocarinatus, E. nobilii and E. crassus, the E. petzi pheromones are smaller (32 amino acids) and richer in Cys residues (eight) located in strictly conserved positions. These residues are predicted to form four intra-chain disulfide bridges, which suggests a compact globular fold of the molecules. However, the NMR solution structure determined for one of the E. petzi pheromones challenges this hypothesis. The structure consists of one more extended eight-residue alpha-helix and one smaller four-residue helix, and shows large polypeptide segments devoid of regular secondary structures. Pheromones from other Euplotes species which live in temperate waters and branch later than E. petzi in the Euplotes phylogenetic tree are known to be characterized by a three-helix fold and unstructured regions of comparatively limited dimensions. In the light of this knowledge, we can thus draw two distinct conclusions from our findings. The first, of phylogenetic nature, is that the structural evolution of the Euplotes pheromones involves an increase in size and complexity. This is in line with the smaller and simpler organization that also the macronuclear E. petzi pheromone genes show with respect to their homologues in other Euplotes species. The second conclusion is that the extended unstructured regions of E. petzi pheromones are likely correlated with an increased flexibility of ... Conference Object Antarc* Antarctic Arctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Arctic Antarctic |
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Open Polar |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
Euplotes species are valuable for the study of the structural and functional biology of water-borne protein pheromones that cells constitutively synthesize and use in intra-specific chemical communication. We have recently devoted particular attention to the pheromone family of the “cold loving” (psychrophilic) species E. petzi which dwells in the freezing Antarctic and Arctic coastal sea waters, and forms, together with E. sinicus, the earliest branch of the Euplotes phylogenetic tree. From cultures of genetically distinct strains, we have isolated and sequenced four E. petzi pheromones. With respect to the known pheromones from E. raikovi, E. octocarinatus, E. nobilii and E. crassus, the E. petzi pheromones are smaller (32 amino acids) and richer in Cys residues (eight) located in strictly conserved positions. These residues are predicted to form four intra-chain disulfide bridges, which suggests a compact globular fold of the molecules. However, the NMR solution structure determined for one of the E. petzi pheromones challenges this hypothesis. The structure consists of one more extended eight-residue alpha-helix and one smaller four-residue helix, and shows large polypeptide segments devoid of regular secondary structures. Pheromones from other Euplotes species which live in temperate waters and branch later than E. petzi in the Euplotes phylogenetic tree are known to be characterized by a three-helix fold and unstructured regions of comparatively limited dimensions. In the light of this knowledge, we can thus draw two distinct conclusions from our findings. The first, of phylogenetic nature, is that the structural evolution of the Euplotes pheromones involves an increase in size and complexity. This is in line with the smaller and simpler organization that also the macronuclear E. petzi pheromone genes show with respect to their homologues in other Euplotes species. The second conclusion is that the extended unstructured regions of E. petzi pheromones are likely correlated with an increased flexibility of ... |
author2 |
Vallesi, Adriana Alimenti, Claudio Bill, Pedrini Luporini, Pierangelo |
format |
Conference Object |
author |
VALLESI, Adriana ALIMENTI, Claudio Bill Pedrini LUPORINI, Pierangelo |
spellingShingle |
VALLESI, Adriana ALIMENTI, Claudio Bill Pedrini LUPORINI, Pierangelo THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
author_facet |
VALLESI, Adriana ALIMENTI, Claudio Bill Pedrini LUPORINI, Pierangelo |
author_sort |
VALLESI, Adriana |
title |
THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
title_short |
THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
title_full |
THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
title_fullStr |
THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
title_full_unstemmed |
THE PROTEIN PHEROMONE FAMILY OF E. PETZI, A PSYCHROPHILIC AND EARLY BRANCHING EUPLOTES SPECIES |
title_sort |
protein pheromone family of e. petzi, a psychrophilic and early branching euplotes species |
publisher |
Universidad de Sevilla |
publishDate |
2015 |
url |
http://hdl.handle.net/11581/391681 http://feps.ciccartuja.es/ecop/ |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
ispartofbook:VII European Congress of Protistology in partnership with The International Society of Protistologists Final programme abstracts VII European Congress of Protistology firstpage:400 lastpage:400 numberofpages:1 http://hdl.handle.net/11581/391681 http://feps.ciccartuja.es/ecop/ |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1789325231199879168 |