SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI
Ciliates of the genus Euplotes are commonly found in polar environments, and different species isolated from Arctic and Antarctic coastal seawaters are currently studied for their genome evolution and adaptation. In analyzing whole genome sequences of a wild-type E. petzi strain collected from Terra...
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Universidad de Sevilla
2015
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/391680 2024-01-28T10:01:15+01:00 SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI VALLESI, Adriana PETRELLI, Dezemona Graziano Di Giuseppe Andreas Sjödin Johanna Thelaus Elin Nilsson Caroline Öhrman Gabriel Gutierrez Pozo Eduardo Villalobo Vallesi, Adriana Petrelli, Dezemona Graziano Di, Giuseppe Andreas, Sjödin Johanna, Thelau Elin, Nilsson Caroline, Öhrman Gabriel Gutierrez, Pozo Eduardo, Villalobo 2015 STAMPA http://hdl.handle.net/11581/391680 http://feps.ciccartuja.es/ecop/ eng eng Universidad de Sevilla country:ESP place:Seville ispartofbook:VII European Congress of Protistology in partnership with The International Society of Protistologists Final programme abstracts VII European Congress of Protistology firstpage:215 lastpage:215 numberofpages:1 http://hdl.handle.net/11581/391680 http://feps.ciccartuja.es/ecop/ info:eu-repo/semantics/openAccess info:eu-repo/semantics/conferenceObject 2015 ftuncamerinoiris 2024-01-03T17:43:30Z Ciliates of the genus Euplotes are commonly found in polar environments, and different species isolated from Arctic and Antarctic coastal seawaters are currently studied for their genome evolution and adaptation. In analyzing whole genome sequences of a wild-type E. petzi strain collected from Terra Nova Bay (Antarctica), it appeared that more than 3% of the assembled contigs had a bacterial origin and overlapped (one contig containing rDNA operon included) with DNA sequences of the gammaproteobacterium Francisella (represented by extremely infectious species to a wide array of different organisms man included). Given that an Euplotes species of temperate seawaters, E. raikovi, has already been found to host a Francisella species (namely F. endociliophora), we searched for and succeeded in isolating Francisella-like endosymbionts from E. petzi cells. Colonies of these endosymbionts (grown optimally at a temperature range from 4 to maximum 30 °C) have been analyzed for their genome and found to represent a new clade with a basal position in the Francisella phylogenetic tree. This clade is unequivocally distinct from F. endociliophora (living in E. raikovi) as well as from all the other well recognised Francisella clades. The finding that Francisella is adapted to live in the extreme environmental conditions of the polar regions implies that this bacterium is much more common and geographically widespread than previously known, and that free-living Euplotes species may represent a natural reservoir of Francisella in every aquatic environments. Conference Object Antarc* Antarctic Antarctica Arctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Arctic Antarctic Terra Nova Bay |
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Open Polar |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
Ciliates of the genus Euplotes are commonly found in polar environments, and different species isolated from Arctic and Antarctic coastal seawaters are currently studied for their genome evolution and adaptation. In analyzing whole genome sequences of a wild-type E. petzi strain collected from Terra Nova Bay (Antarctica), it appeared that more than 3% of the assembled contigs had a bacterial origin and overlapped (one contig containing rDNA operon included) with DNA sequences of the gammaproteobacterium Francisella (represented by extremely infectious species to a wide array of different organisms man included). Given that an Euplotes species of temperate seawaters, E. raikovi, has already been found to host a Francisella species (namely F. endociliophora), we searched for and succeeded in isolating Francisella-like endosymbionts from E. petzi cells. Colonies of these endosymbionts (grown optimally at a temperature range from 4 to maximum 30 °C) have been analyzed for their genome and found to represent a new clade with a basal position in the Francisella phylogenetic tree. This clade is unequivocally distinct from F. endociliophora (living in E. raikovi) as well as from all the other well recognised Francisella clades. The finding that Francisella is adapted to live in the extreme environmental conditions of the polar regions implies that this bacterium is much more common and geographically widespread than previously known, and that free-living Euplotes species may represent a natural reservoir of Francisella in every aquatic environments. |
author2 |
Vallesi, Adriana Petrelli, Dezemona Graziano Di, Giuseppe Andreas, Sjödin Johanna, Thelau Elin, Nilsson Caroline, Öhrman Gabriel Gutierrez, Pozo Eduardo, Villalobo |
format |
Conference Object |
author |
VALLESI, Adriana PETRELLI, Dezemona Graziano Di Giuseppe Andreas Sjödin Johanna Thelaus Elin Nilsson Caroline Öhrman Gabriel Gutierrez Pozo Eduardo Villalobo |
spellingShingle |
VALLESI, Adriana PETRELLI, Dezemona Graziano Di Giuseppe Andreas Sjödin Johanna Thelaus Elin Nilsson Caroline Öhrman Gabriel Gutierrez Pozo Eduardo Villalobo SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
author_facet |
VALLESI, Adriana PETRELLI, Dezemona Graziano Di Giuseppe Andreas Sjödin Johanna Thelaus Elin Nilsson Caroline Öhrman Gabriel Gutierrez Pozo Eduardo Villalobo |
author_sort |
VALLESI, Adriana |
title |
SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
title_short |
SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
title_full |
SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
title_fullStr |
SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
title_full_unstemmed |
SYMBIOSIS IN THE COLD: IDENTIFICATION AND CHARACTERIZATION OF A NEW FRANCISELLA ENDOSYMBIONT FROM THE POLAR CILIATE, EUPLOTES PETZI |
title_sort |
symbiosis in the cold: identification and characterization of a new francisella endosymbiont from the polar ciliate, euplotes petzi |
publisher |
Universidad de Sevilla |
publishDate |
2015 |
url |
http://hdl.handle.net/11581/391680 http://feps.ciccartuja.es/ecop/ |
geographic |
Arctic Antarctic Terra Nova Bay |
geographic_facet |
Arctic Antarctic Terra Nova Bay |
genre |
Antarc* Antarctic Antarctica Arctic |
genre_facet |
Antarc* Antarctic Antarctica Arctic |
op_relation |
ispartofbook:VII European Congress of Protistology in partnership with The International Society of Protistologists Final programme abstracts VII European Congress of Protistology firstpage:215 lastpage:215 numberofpages:1 http://hdl.handle.net/11581/391680 http://feps.ciccartuja.es/ecop/ |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1789326066886639616 |