Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii
We identified two ice-binding protein (IBP) sequences, named EFsymbAFP and EFsymbIBP, from a putative bacterial symbiont of the Antarctic psychrophilic ciliate Euplotes focardii. EFsymbAFP is 57,43% identical to the antifreeze protein (AFP) from the Stigmatella aurantiaca strain DW4/3-1, which was i...
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Online Access: | http://hdl.handle.net/11581/305983 https://doi.org/10.1017/S0954102014000017 |
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/305983 2024-04-14T08:01:27+00:00 Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Federica Chiappori Raghul Rajan Devaraj Guang Yang Ting Yu Pucciarelli, Sandra Federica, Chiappori Raghul Rajan, Devaraj Guang, Yang Ting, Yu Ballarini, Patrizia Miceli, Cristina 2014 ELETTRONICO http://hdl.handle.net/11581/305983 https://doi.org/10.1017/S0954102014000017 eng eng volume:FirstView issue:Supplement -1 firstpage:1 lastpage:11 numberofpages:11 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11581/305983 doi:10.1017/S0954102014000017 info:eu-repo/semantics/altIdentifier/scopus/000344735300004 info:eu-repo/semantics/article 2014 ftuncamerinoiris https://doi.org/10.1017/S0954102014000017 2024-03-21T20:36:53Z We identified two ice-binding protein (IBP) sequences, named EFsymbAFP and EFsymbIBP, from a putative bacterial symbiont of the Antarctic psychrophilic ciliate Euplotes focardii. EFsymbAFP is 57,43% identical to the antifreeze protein (AFP) from the Stigmatella aurantiaca strain DW4/3-1, which was isolated from the Lower Victoria valley glacier (Antarctica). EFsymbIBP is 53,38 % identical to the IBP from the Flavobacteriaceae bacterium strain 3519-10, isolated from the glacial ice of the Lake Vostok. EFsymbAFP and EFsymbIBP are 31,73% identical at the amino acid level and are organised in tandem on the bacterial chromosome. The relatively low sequence identity and the tandem organisation, which appears unique to this symbiont, suggest an occurrence of horizontal gene transfer. Structurally, EFsymbAFP and EFsymbIBP are similar to the AFPs from the snow mould fungus Typhula ishikariensis and from the Arctic yeast Leucosporidium sp. AY30. A phylogenetic analysis showed that EFsymbAFP and EFsymbIBP cluster principally with the IBPs sequences from other Antarctic bacteria, supporting the view that these sequences belong to an Antarctic symbiontic bacterium of E. focardii. These results confirm that IBPs have a complex evolutionary history, which includes horizontal gene transfer events, most likely due to the demands of the environment and the need for rapid adaptation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Arctic Antarctic The Antarctic Lake Vostok ENVELOPE(106.000,106.000,-77.500,-77.500) Victoria Valley ENVELOPE(162.000,162.000,-77.383,-77.383) Antarctic Science 26 5 491 501 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
We identified two ice-binding protein (IBP) sequences, named EFsymbAFP and EFsymbIBP, from a putative bacterial symbiont of the Antarctic psychrophilic ciliate Euplotes focardii. EFsymbAFP is 57,43% identical to the antifreeze protein (AFP) from the Stigmatella aurantiaca strain DW4/3-1, which was isolated from the Lower Victoria valley glacier (Antarctica). EFsymbIBP is 53,38 % identical to the IBP from the Flavobacteriaceae bacterium strain 3519-10, isolated from the glacial ice of the Lake Vostok. EFsymbAFP and EFsymbIBP are 31,73% identical at the amino acid level and are organised in tandem on the bacterial chromosome. The relatively low sequence identity and the tandem organisation, which appears unique to this symbiont, suggest an occurrence of horizontal gene transfer. Structurally, EFsymbAFP and EFsymbIBP are similar to the AFPs from the snow mould fungus Typhula ishikariensis and from the Arctic yeast Leucosporidium sp. AY30. A phylogenetic analysis showed that EFsymbAFP and EFsymbIBP cluster principally with the IBPs sequences from other Antarctic bacteria, supporting the view that these sequences belong to an Antarctic symbiontic bacterium of E. focardii. These results confirm that IBPs have a complex evolutionary history, which includes horizontal gene transfer events, most likely due to the demands of the environment and the need for rapid adaptation. |
author2 |
Pucciarelli, Sandra Federica, Chiappori Raghul Rajan, Devaraj Guang, Yang Ting, Yu Ballarini, Patrizia Miceli, Cristina |
format |
Article in Journal/Newspaper |
author |
PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Federica Chiappori Raghul Rajan Devaraj Guang Yang Ting Yu |
spellingShingle |
PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Federica Chiappori Raghul Rajan Devaraj Guang Yang Ting Yu Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
author_facet |
PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Federica Chiappori Raghul Rajan Devaraj Guang Yang Ting Yu |
author_sort |
PUCCIARELLI, Sandra |
title |
Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
title_short |
Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
title_full |
Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
title_fullStr |
Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
title_full_unstemmed |
Identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic Antarctic ciliate Euplotes focardii |
title_sort |
identification and analysis of two sequences encoding ice-binding proteins obtained from a putative bacterial symbiont of the psychrophilic antarctic ciliate euplotes focardii |
publishDate |
2014 |
url |
http://hdl.handle.net/11581/305983 https://doi.org/10.1017/S0954102014000017 |
long_lat |
ENVELOPE(106.000,106.000,-77.500,-77.500) ENVELOPE(162.000,162.000,-77.383,-77.383) |
geographic |
Arctic Antarctic The Antarctic Lake Vostok Victoria Valley |
geographic_facet |
Arctic Antarctic The Antarctic Lake Vostok Victoria Valley |
genre |
Antarc* Antarctic Antarctica Arctic |
genre_facet |
Antarc* Antarctic Antarctica Arctic |
op_relation |
volume:FirstView issue:Supplement -1 firstpage:1 lastpage:11 numberofpages:11 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11581/305983 doi:10.1017/S0954102014000017 info:eu-repo/semantics/altIdentifier/scopus/000344735300004 |
op_doi |
https://doi.org/10.1017/S0954102014000017 |
container_title |
Antarctic Science |
container_volume |
26 |
container_issue |
5 |
container_start_page |
491 |
op_container_end_page |
501 |
_version_ |
1796309059026026496 |