Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii
In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was...
Published in: | International Journal of Molecular Sciences |
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Online Access: | http://hdl.handle.net/11581/265216 https://doi.org/10.3390/ijms14047457 |
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/265216 2024-04-14T08:04:01+00:00 Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii CANDELORI, ANNALISA LUPORINI, Pierangelo ALIMENTI, Claudio VALLESI, Adriana Candelori, Annalisa Luporini, Pierangelo Alimenti, Claudio Vallesi, Adriana 2013 http://hdl.handle.net/11581/265216 https://doi.org/10.3390/ijms14047457 eng eng info:eu-repo/semantics/altIdentifier/wos/000318017100045 volume:14 issue:4 firstpage:7457 lastpage:7467 numberofpages:11 journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES http://hdl.handle.net/11581/265216 doi:10.3390/ijms14047457 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84876009311 info:eu-repo/semantics/article 2013 ftuncamerinoiris https://doi.org/10.3390/ijms14047457 2024-03-21T20:38:18Z In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation. Article in Journal/Newspaper Antarc* Antarctic Arctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Arctic Antarctic International Journal of Molecular Sciences 14 4 7457 7467 |
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Open Polar |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
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ftuncamerinoiris |
language |
English |
description |
In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation. |
author2 |
Candelori, Annalisa Luporini, Pierangelo Alimenti, Claudio Vallesi, Adriana |
format |
Article in Journal/Newspaper |
author |
CANDELORI, ANNALISA LUPORINI, Pierangelo ALIMENTI, Claudio VALLESI, Adriana |
spellingShingle |
CANDELORI, ANNALISA LUPORINI, Pierangelo ALIMENTI, Claudio VALLESI, Adriana Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
author_facet |
CANDELORI, ANNALISA LUPORINI, Pierangelo ALIMENTI, Claudio VALLESI, Adriana |
author_sort |
CANDELORI, ANNALISA |
title |
Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
title_short |
Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
title_full |
Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
title_fullStr |
Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
title_full_unstemmed |
Characterization and Expression of the Gene Encoding En-MAPK1, an Intestinal Cell Kinase (ICK)-like Kinase Activated by the Autocrine Pheromone-Signaling Loop in the Polar Ciliate, Euplotes nobilii |
title_sort |
characterization and expression of the gene encoding en-mapk1, an intestinal cell kinase (ick)-like kinase activated by the autocrine pheromone-signaling loop in the polar ciliate, euplotes nobilii |
publishDate |
2013 |
url |
http://hdl.handle.net/11581/265216 https://doi.org/10.3390/ijms14047457 |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/000318017100045 volume:14 issue:4 firstpage:7457 lastpage:7467 numberofpages:11 journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES http://hdl.handle.net/11581/265216 doi:10.3390/ijms14047457 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84876009311 |
op_doi |
https://doi.org/10.3390/ijms14047457 |
container_title |
International Journal of Molecular Sciences |
container_volume |
14 |
container_issue |
4 |
container_start_page |
7457 |
op_container_end_page |
7467 |
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1796300367426748416 |