The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate

In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, str...

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Published in:Antarctic Science
Main Authors: La Terza, Antonietta, Miceli, Cristina, Luporini, Pierangelo
Format: Article in Journal/Newspaper
Language:English
Published: 2004
Subjects:
Online Access:http://hdl.handle.net/11581/239164
https://doi.org/10.1017/S0954102004001774
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spelling ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/239164 2024-04-14T08:02:20+00:00 The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo 2004 STAMPA http://hdl.handle.net/11581/239164 https://doi.org/10.1017/S0954102004001774 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000220310700006 volume:16 issue:1 firstpage:23 lastpage:28 numberofpages:6 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11581/239164 doi:10.1017/S0954102004001774 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1542378751 info:eu-repo/semantics/article 2004 ftuncamerinoiris https://doi.org/10.1017/S0954102004001774 2024-03-21T20:37:07Z In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5′ regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as "Heat Shock Elements" (HSE) and "Stress Response Elements" (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic Antarctic Science 16 1 23 28
institution Open Polar
collection CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino)
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language English
description In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5′ regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as "Heat Shock Elements" (HSE) and "Stress Response Elements" (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses.
author2 La Terza, Antonietta
Miceli, Cristina
Luporini, Pierangelo
format Article in Journal/Newspaper
author La Terza, Antonietta
Miceli, Cristina
Luporini, Pierangelo
spellingShingle La Terza, Antonietta
Miceli, Cristina
Luporini, Pierangelo
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
author_facet La Terza, Antonietta
Miceli, Cristina
Luporini, Pierangelo
author_sort La Terza, Antonietta
title The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
title_short The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
title_full The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
title_fullStr The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
title_full_unstemmed The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
title_sort gene for the heat-shock protein 70 of euplotes focardii, an antarctic psychrophilic ciliate
publishDate 2004
url http://hdl.handle.net/11581/239164
https://doi.org/10.1017/S0954102004001774
geographic Antarctic
The Antarctic
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The Antarctic
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Antarctic
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Antarctic
op_relation info:eu-repo/semantics/altIdentifier/wos/WOS:000220310700006
volume:16
issue:1
firstpage:23
lastpage:28
numberofpages:6
journal:ANTARCTIC SCIENCE
http://hdl.handle.net/11581/239164
doi:10.1017/S0954102004001774
info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1542378751
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