The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, str...
Published in: | Antarctic Science |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2004
|
Subjects: | |
Online Access: | http://hdl.handle.net/11581/239164 https://doi.org/10.1017/S0954102004001774 |
id |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/239164 |
---|---|
record_format |
openpolar |
spelling |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/239164 2024-04-14T08:02:20+00:00 The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo 2004 STAMPA http://hdl.handle.net/11581/239164 https://doi.org/10.1017/S0954102004001774 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000220310700006 volume:16 issue:1 firstpage:23 lastpage:28 numberofpages:6 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11581/239164 doi:10.1017/S0954102004001774 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1542378751 info:eu-repo/semantics/article 2004 ftuncamerinoiris https://doi.org/10.1017/S0954102004001774 2024-03-21T20:37:07Z In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5′ regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as "Heat Shock Elements" (HSE) and "Stress Response Elements" (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic Antarctic Science 16 1 23 28 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
In the Antarctic ciliate, Euplotes focardii, the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5′ regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as "Heat Shock Elements" (HSE) and "Stress Response Elements" (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses. |
author2 |
La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo |
format |
Article in Journal/Newspaper |
author |
La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo |
spellingShingle |
La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
author_facet |
La Terza, Antonietta Miceli, Cristina Luporini, Pierangelo |
author_sort |
La Terza, Antonietta |
title |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_short |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_full |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_fullStr |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_full_unstemmed |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_sort |
gene for the heat-shock protein 70 of euplotes focardii, an antarctic psychrophilic ciliate |
publishDate |
2004 |
url |
http://hdl.handle.net/11581/239164 https://doi.org/10.1017/S0954102004001774 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000220310700006 volume:16 issue:1 firstpage:23 lastpage:28 numberofpages:6 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11581/239164 doi:10.1017/S0954102004001774 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-1542378751 |
op_doi |
https://doi.org/10.1017/S0954102004001774 |
container_title |
Antarctic Science |
container_volume |
16 |
container_issue |
1 |
container_start_page |
23 |
op_container_end_page |
28 |
_version_ |
1796314158052933632 |