Molecular assay for the identification of Setaria tundra
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this coun...
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2003
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Online Access: | http://hdl.handle.net/11581/116533 |
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/116533 2024-09-15T18:39:34+00:00 Molecular assay for the identification of Setaria tundra FAVIA, GUIDO RICCI, Irene CANCRINI G. FERROGLIO E. CASIRAGHI M. ROSSI L. Favia, Guido Cancrini, G. Ferroglio, E. Casiraghi, M. Ricci, Irene Rossi, L. 2003 STAMPA http://hdl.handle.net/11581/116533 eng eng volume:117 issue:1-2 firstpage:139 lastpage:145 numberofpages:7 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/11581/116533 info:eu-repo/semantics/article 2003 ftuncamerinoiris 2024-06-24T23:58:56Z We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. Article in Journal/Newspaper Tundra CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
We report the molecular characterization of the amplification products obtained by specific PCR experiments aimed to identify the 5S ribosomal spacer of Setaria tundra specimens isolated from roe deer (Capreolus capreolus) in north Italy, which represent the first record of the parasite in this country. Three different fragments of approximately 400, 800, and 1200 bp (base pairs) are produced. Sequence analyses showed that all three fragments share a very high level of similarity to 5S spacer sequences of some Setaria species and other filariae present in genebank. Based on these sequences, we were able to design species-specific PCR primers for the precise identification of S. tundra. |
author2 |
Favia, Guido Cancrini, G. Ferroglio, E. Casiraghi, M. Ricci, Irene Rossi, L. |
format |
Article in Journal/Newspaper |
author |
FAVIA, GUIDO RICCI, Irene CANCRINI G. FERROGLIO E. CASIRAGHI M. ROSSI L. |
spellingShingle |
FAVIA, GUIDO RICCI, Irene CANCRINI G. FERROGLIO E. CASIRAGHI M. ROSSI L. Molecular assay for the identification of Setaria tundra |
author_facet |
FAVIA, GUIDO RICCI, Irene CANCRINI G. FERROGLIO E. CASIRAGHI M. ROSSI L. |
author_sort |
FAVIA, GUIDO |
title |
Molecular assay for the identification of Setaria tundra |
title_short |
Molecular assay for the identification of Setaria tundra |
title_full |
Molecular assay for the identification of Setaria tundra |
title_fullStr |
Molecular assay for the identification of Setaria tundra |
title_full_unstemmed |
Molecular assay for the identification of Setaria tundra |
title_sort |
molecular assay for the identification of setaria tundra |
publishDate |
2003 |
url |
http://hdl.handle.net/11581/116533 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
volume:117 issue:1-2 firstpage:139 lastpage:145 numberofpages:7 journal:VETERINARY PARASITOLOGY http://hdl.handle.net/11581/116533 |
_version_ |
1810483928712085504 |