Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii
Tubulin dimers of psychrophilic organisms can polymerize into microtubules at temperatures below 4 degrees C, at which non-cold-adapted microtubules disassemble. This capacity requires specificities in the structure and/or in the posttranslational modifications of the tubulin subunits. A contributio...
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Online Access: | http://hdl.handle.net/11581/116426 https://doi.org/10.1007/s00792-002-0268-5 https://link.springer.com/article/10.1007/s00792-002-0268-5 |
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/116426 2024-04-14T08:02:05+00:00 Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii PUCCIARELLI, Sandra MICELI, Cristina Pucciarelli, Sandra Miceli, Cristina 2002 ELETTRONICO http://hdl.handle.net/11581/116426 https://doi.org/10.1007/s00792-002-0268-5 https://link.springer.com/article/10.1007/s00792-002-0268-5 eng eng info:eu-repo/semantics/altIdentifier/pmid/12382114 info:eu-repo/semantics/altIdentifier/wos/WOS:000178680700005 volume:6 issue:5 firstpage:385 lastpage:389 numberofpages:5 journal:EXTREMOPHILES http://hdl.handle.net/11581/116426 doi:10.1007/s00792-002-0268-5 https://link.springer.com/article/10.1007/s00792-002-0268-5 info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2002 ftuncamerinoiris https://doi.org/10.1007/s00792-002-0268-5 2024-03-21T20:36:40Z Tubulin dimers of psychrophilic organisms can polymerize into microtubules at temperatures below 4 degrees C, at which non-cold-adapted microtubules disassemble. This capacity requires specificities in the structure and/or in the posttranslational modifications of the tubulin subunits. A contribution to the knowledge of these specificities was provided by the finding that the amino acid sequence of the alpha-tubulin of the Antarctic ciliate Euplotes focardii contains substitutions that, in addition to conferring an increased hydrophobicity to the molecule, modify sites that are involved in alpha-/alpha-tubulin lateral contacts between protofilaments. At the level of the coding sequence, the alpha-tubulin gene of E. focardii revealed an A+T content appreciably higher than in its homologs in ciliates of temperate waters. This was interpreted as an adaptation to favor DNA strand separation in an environment which is energetically adverse. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic Extremophiles 6 5 385 389 |
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Open Polar |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
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ftuncamerinoiris |
language |
English |
description |
Tubulin dimers of psychrophilic organisms can polymerize into microtubules at temperatures below 4 degrees C, at which non-cold-adapted microtubules disassemble. This capacity requires specificities in the structure and/or in the posttranslational modifications of the tubulin subunits. A contribution to the knowledge of these specificities was provided by the finding that the amino acid sequence of the alpha-tubulin of the Antarctic ciliate Euplotes focardii contains substitutions that, in addition to conferring an increased hydrophobicity to the molecule, modify sites that are involved in alpha-/alpha-tubulin lateral contacts between protofilaments. At the level of the coding sequence, the alpha-tubulin gene of E. focardii revealed an A+T content appreciably higher than in its homologs in ciliates of temperate waters. This was interpreted as an adaptation to favor DNA strand separation in an environment which is energetically adverse. |
author2 |
Pucciarelli, Sandra Miceli, Cristina |
format |
Article in Journal/Newspaper |
author |
PUCCIARELLI, Sandra MICELI, Cristina |
spellingShingle |
PUCCIARELLI, Sandra MICELI, Cristina Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
author_facet |
PUCCIARELLI, Sandra MICELI, Cristina |
author_sort |
PUCCIARELLI, Sandra |
title |
Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
title_short |
Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
title_full |
Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
title_fullStr |
Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
title_full_unstemmed |
Characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate Euplotes focardii |
title_sort |
characterization of the cold-adapted -alpha-tubulin from the psychrophilic ciliate euplotes focardii |
publishDate |
2002 |
url |
http://hdl.handle.net/11581/116426 https://doi.org/10.1007/s00792-002-0268-5 https://link.springer.com/article/10.1007/s00792-002-0268-5 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12382114 info:eu-repo/semantics/altIdentifier/wos/WOS:000178680700005 volume:6 issue:5 firstpage:385 lastpage:389 numberofpages:5 journal:EXTREMOPHILES http://hdl.handle.net/11581/116426 doi:10.1007/s00792-002-0268-5 https://link.springer.com/article/10.1007/s00792-002-0268-5 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00792-002-0268-5 |
container_title |
Extremophiles |
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6 |
container_issue |
5 |
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385 |
op_container_end_page |
389 |
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1796312610463809536 |