The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone
The marine Antarctic ciliate, Euplotes nobilii, secretes a family of water-borne signal proteins, denoted as pheromones, that control the cell vegetative proliferation and mating. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernatant of...
Published in: | Canadian Journal of Microbiology |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2009
|
Subjects: | |
Online Access: | http://hdl.handle.net/11581/115119 https://doi.org/10.1139/W08-122 |
id |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/115119 |
---|---|
record_format |
openpolar |
spelling |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/115119 2024-04-14T08:01:57+00:00 The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone LA TERZA, Antonietta DOBRI, NICOLETA ALIMENTI, Claudio VALLESI, Adriana LUPORINI, Pierangelo LA TERZA, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo 2009 STAMPA http://hdl.handle.net/11581/115119 https://doi.org/10.1139/W08-122 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000263468500007 volume:55 issue:1 firstpage:57 lastpage:62 numberofpages:6 journal:CANADIAN JOURNAL OF MICROBIOLOGY http://hdl.handle.net/11581/115119 doi:10.1139/W08-122 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-64249110661 info:eu-repo/semantics/article 2009 ftuncamerinoiris https://doi.org/10.1139/W08-122 2024-03-21T20:37:40Z The marine Antarctic ciliate, Euplotes nobilii, secretes a family of water-borne signal proteins, denoted as pheromones, that control the cell vegetative proliferation and mating. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernatant of wild-type strains, we designed probes to identify their encoding genes represented by linear sub-chromosomic (gene-sized) DNA molecules in the somatic nucleus (macronucleus) of the cell. The full-length gene of the pheromone En-6 was determined and found to contain an open reading frame specific for the synthesis of the En-6 cytoplasmic precursor (pre-pro-En-6), that requires two proteolytic cleavages to remove the signal-peptide (pre) and the pro segment before secretion of the mature protein. In contrast to from the sequence variability that distinguishes the secreted pheromones, the pre and pro sequences appear to be tightly conserved and useful to construct probes to clone every other E. nobilii pheromone gene. Potential intron sequences in the coding region of the En-6 gene imply the synthesis of more En-6 isoforms. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic Canadian Journal of Microbiology 55 1 57 62 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
The marine Antarctic ciliate, Euplotes nobilii, secretes a family of water-borne signal proteins, denoted as pheromones, that control the cell vegetative proliferation and mating. Based on the knowledge of the amino acid sequences of a set of these pheromones isolated from the culture supernatant of wild-type strains, we designed probes to identify their encoding genes represented by linear sub-chromosomic (gene-sized) DNA molecules in the somatic nucleus (macronucleus) of the cell. The full-length gene of the pheromone En-6 was determined and found to contain an open reading frame specific for the synthesis of the En-6 cytoplasmic precursor (pre-pro-En-6), that requires two proteolytic cleavages to remove the signal-peptide (pre) and the pro segment before secretion of the mature protein. In contrast to from the sequence variability that distinguishes the secreted pheromones, the pre and pro sequences appear to be tightly conserved and useful to construct probes to clone every other E. nobilii pheromone gene. Potential intron sequences in the coding region of the En-6 gene imply the synthesis of more En-6 isoforms. |
author2 |
LA TERZA, Antonietta Dobri, Nicoleta Alimenti, Claudio Vallesi, Adriana Luporini, Pierangelo |
format |
Article in Journal/Newspaper |
author |
LA TERZA, Antonietta DOBRI, NICOLETA ALIMENTI, Claudio VALLESI, Adriana LUPORINI, Pierangelo |
spellingShingle |
LA TERZA, Antonietta DOBRI, NICOLETA ALIMENTI, Claudio VALLESI, Adriana LUPORINI, Pierangelo The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
author_facet |
LA TERZA, Antonietta DOBRI, NICOLETA ALIMENTI, Claudio VALLESI, Adriana LUPORINI, Pierangelo |
author_sort |
LA TERZA, Antonietta |
title |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
title_short |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
title_full |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
title_fullStr |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
title_full_unstemmed |
The water-borne protein signals (pheromones) of the Antarctic ciliated protozoan, Euplotes nobilii: structure of the gene coding for the En-6 pheromone |
title_sort |
water-borne protein signals (pheromones) of the antarctic ciliated protozoan, euplotes nobilii: structure of the gene coding for the en-6 pheromone |
publishDate |
2009 |
url |
http://hdl.handle.net/11581/115119 https://doi.org/10.1139/W08-122 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000263468500007 volume:55 issue:1 firstpage:57 lastpage:62 numberofpages:6 journal:CANADIAN JOURNAL OF MICROBIOLOGY http://hdl.handle.net/11581/115119 doi:10.1139/W08-122 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-64249110661 |
op_doi |
https://doi.org/10.1139/W08-122 |
container_title |
Canadian Journal of Microbiology |
container_volume |
55 |
container_issue |
1 |
container_start_page |
57 |
op_container_end_page |
62 |
_version_ |
1796311853217873920 |