Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature
In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). c-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of c-tubu...
Published in: | FEBS Journal |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2008
|
Subjects: | |
Online Access: | http://hdl.handle.net/11581/112520 https://doi.org/10.1111/j.1742-4658.2008.06666.x |
id |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/112520 |
---|---|
record_format |
openpolar |
spelling |
ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/112520 2023-05-15T13:50:54+02:00 Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature MARZIALE F MELKI R UZUN A ILYIN VA DETRICH HW PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Marziale, F Pucciarelli, Sandra Ballarini, Patrizia Melki, R Uzun, A Ilyin, Va Detrich, Hw Miceli, Cristina 2008 http://hdl.handle.net/11581/112520 https://doi.org/10.1111/j.1742-4658.2008.06666.x eng eng volume:275 issue:21 firstpage:5367 lastpage:5382 numberofpages:16 journal:THE FEBS JOURNAL http://hdl.handle.net/11581/112520 doi:10.1111/j.1742-4658.2008.06666.x info:eu-repo/semantics/article 2008 ftuncamerinoiris https://doi.org/10.1111/j.1742-4658.2008.06666.x 2023-03-01T21:00:51Z In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). c-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of c-tubulin on different MTOCs and during the cell cycle. In Tetrahymena thermophila, the a- and b-tubulin expression is regulated mainly at the transcriptional level, and changes in the ratio of polymerized/unpolymerized tubulin dimers lead to an increase or decrease of a- and b-tubulin transcription. This study deals with the characterization of c-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Sequence analysis revealed some specific substitutions in nucleotidebinding loops characteristic of the Tetrahymena genus and putative conserved phosphorylation sites located on the external surface of the c-tubulin molecule. c-Tubulin expression during the cell cycle, in the presence of microtubular poisons and after deciliation, was also characterized. We found that c-tubulin mRNA levels are correlated with basal body proliferation and c-tubulin nuclear localization. We also found that c-tubulin expression changes during anti-microtubular drugs treatment, but does not changes during reciliation. These findings suggest a relationship between the level of unpolymerized tubulin dimers and c-tubulin transcription. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic The Antarctic FEBS Journal 275 21 5367 5382 |
institution |
Open Polar |
collection |
CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
op_collection_id |
ftuncamerinoiris |
language |
English |
description |
In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). c-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of c-tubulin on different MTOCs and during the cell cycle. In Tetrahymena thermophila, the a- and b-tubulin expression is regulated mainly at the transcriptional level, and changes in the ratio of polymerized/unpolymerized tubulin dimers lead to an increase or decrease of a- and b-tubulin transcription. This study deals with the characterization of c-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Sequence analysis revealed some specific substitutions in nucleotidebinding loops characteristic of the Tetrahymena genus and putative conserved phosphorylation sites located on the external surface of the c-tubulin molecule. c-Tubulin expression during the cell cycle, in the presence of microtubular poisons and after deciliation, was also characterized. We found that c-tubulin mRNA levels are correlated with basal body proliferation and c-tubulin nuclear localization. We also found that c-tubulin expression changes during anti-microtubular drugs treatment, but does not changes during reciliation. These findings suggest a relationship between the level of unpolymerized tubulin dimers and c-tubulin transcription. |
author2 |
Marziale, F Pucciarelli, Sandra Ballarini, Patrizia Melki, R Uzun, A Ilyin, Va Detrich, Hw Miceli, Cristina |
format |
Article in Journal/Newspaper |
author |
MARZIALE F MELKI R UZUN A ILYIN VA DETRICH HW PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina |
spellingShingle |
MARZIALE F MELKI R UZUN A ILYIN VA DETRICH HW PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
author_facet |
MARZIALE F MELKI R UZUN A ILYIN VA DETRICH HW PUCCIARELLI, Sandra BALLARINI, Patrizia MICELI, Cristina |
author_sort |
MARZIALE F |
title |
Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
title_short |
Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
title_full |
Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
title_fullStr |
Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
title_full_unstemmed |
Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
title_sort |
different roles of two gamma-tubulin isotypes in the cytoskeleton of the antarctic ciliate euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature |
publishDate |
2008 |
url |
http://hdl.handle.net/11581/112520 https://doi.org/10.1111/j.1742-4658.2008.06666.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
volume:275 issue:21 firstpage:5367 lastpage:5382 numberofpages:16 journal:THE FEBS JOURNAL http://hdl.handle.net/11581/112520 doi:10.1111/j.1742-4658.2008.06666.x |
op_doi |
https://doi.org/10.1111/j.1742-4658.2008.06666.x |
container_title |
FEBS Journal |
container_volume |
275 |
container_issue |
21 |
container_start_page |
5367 |
op_container_end_page |
5382 |
_version_ |
1766254273341947904 |