Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes
Among Teleosts, Sparus aurata occupies a prominent place in the gastronomic and economic fields of the Mediterranean basin and other geographic districts. The knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mech...
Published in: | OMICS: A Journal of Integrative Biology |
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Online Access: | http://hdl.handle.net/11581/106332 https://doi.org/10.1089/omi.2009.0098 |
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ftuncamerinoiris:oai:pubblicazioni.unicam.it:11581/106332 2023-05-15T13:39:10+02:00 Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes CAMPO S NASTASI G DASCOLA A CAMPO G. M AVENOSO A FERLAZZO A CALATRONI A FEDELI, Donatella FALCIONI, Giancarlo Campo, S Nastasi, G Fedeli, Donatella Dascola, A CAMPO G., M Avenoso, A Ferlazzo, A Calatroni, A Falcioni, Giancarlo 2010 http://hdl.handle.net/11581/106332 https://doi.org/10.1089/omi.2009.0098 eng eng volume:14 issue:2 firstpage:187 lastpage:200 numberofpages:14 journal:OMICS http://hdl.handle.net/11581/106332 doi:10.1089/omi.2009.0098 info:eu-repo/semantics/article 2010 ftuncamerinoiris https://doi.org/10.1089/omi.2009.0098 2023-03-01T21:03:32Z Among Teleosts, Sparus aurata occupies a prominent place in the gastronomic and economic fields of the Mediterranean basin and other geographic districts. The knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mechanisms that allow this fish to live under extreme conditions, including fish farming. In Sparus aurata red blood cells two different alpha and one beta hemoglobin genes have been identified. The alpha1 gene codifies a putative protein of 144 amino acids, the alpha2 gene produces a protein of 143 amino acids, and the beta gene encodes a chain of 148 amino acids. Comparative analysis of various hemoglobins indicates that allosteric regulation can be modified by the substitution of one or a few key residues. The comparison of the percentage sequence differences for alpha and beta chains in fishes indicates that evolutionary relationships between different species may be helpful to understand the mechanisms of their differentiation from other vertebrates. Hemoglobin alpha and beta chains of about 50 teleostean temperate and Antarctic fishes were analyzed to build phylogenetic trees using different algorithms: the neighbor-joining method, the maximum likelihood approach, and the Bayesian inference computation. Sparus aurata alpha chains are positioned in a paraphyletic cluster, which includes the same subunit of Chrysophrys auratus and Seriola quinqueradiata, whereas the beta chain is on an homophyletic branch with that of Chrysophrys auratus. Therefore, the phylogenetic approach suggests that both Sparus aurata hemoglobin alpha genes are paralogues and may have derived from a duplication event. Article in Journal/Newspaper Antarc* Antarctic CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) Antarctic OMICS: A Journal of Integrative Biology 14 2 187 200 |
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CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
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ftuncamerinoiris |
language |
English |
description |
Among Teleosts, Sparus aurata occupies a prominent place in the gastronomic and economic fields of the Mediterranean basin and other geographic districts. The knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mechanisms that allow this fish to live under extreme conditions, including fish farming. In Sparus aurata red blood cells two different alpha and one beta hemoglobin genes have been identified. The alpha1 gene codifies a putative protein of 144 amino acids, the alpha2 gene produces a protein of 143 amino acids, and the beta gene encodes a chain of 148 amino acids. Comparative analysis of various hemoglobins indicates that allosteric regulation can be modified by the substitution of one or a few key residues. The comparison of the percentage sequence differences for alpha and beta chains in fishes indicates that evolutionary relationships between different species may be helpful to understand the mechanisms of their differentiation from other vertebrates. Hemoglobin alpha and beta chains of about 50 teleostean temperate and Antarctic fishes were analyzed to build phylogenetic trees using different algorithms: the neighbor-joining method, the maximum likelihood approach, and the Bayesian inference computation. Sparus aurata alpha chains are positioned in a paraphyletic cluster, which includes the same subunit of Chrysophrys auratus and Seriola quinqueradiata, whereas the beta chain is on an homophyletic branch with that of Chrysophrys auratus. Therefore, the phylogenetic approach suggests that both Sparus aurata hemoglobin alpha genes are paralogues and may have derived from a duplication event. |
author2 |
Campo, S Nastasi, G Fedeli, Donatella Dascola, A CAMPO G., M Avenoso, A Ferlazzo, A Calatroni, A Falcioni, Giancarlo |
format |
Article in Journal/Newspaper |
author |
CAMPO S NASTASI G DASCOLA A CAMPO G. M AVENOSO A FERLAZZO A CALATRONI A FEDELI, Donatella FALCIONI, Giancarlo |
spellingShingle |
CAMPO S NASTASI G DASCOLA A CAMPO G. M AVENOSO A FERLAZZO A CALATRONI A FEDELI, Donatella FALCIONI, Giancarlo Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
author_facet |
CAMPO S NASTASI G DASCOLA A CAMPO G. M AVENOSO A FERLAZZO A CALATRONI A FEDELI, Donatella FALCIONI, Giancarlo |
author_sort |
CAMPO S |
title |
Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
title_short |
Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
title_full |
Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
title_fullStr |
Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
title_full_unstemmed |
Molecular Cloning and Characterization of Adult Sparus aurata Haemoglobin Genes |
title_sort |
molecular cloning and characterization of adult sparus aurata haemoglobin genes |
publishDate |
2010 |
url |
http://hdl.handle.net/11581/106332 https://doi.org/10.1089/omi.2009.0098 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
volume:14 issue:2 firstpage:187 lastpage:200 numberofpages:14 journal:OMICS http://hdl.handle.net/11581/106332 doi:10.1089/omi.2009.0098 |
op_doi |
https://doi.org/10.1089/omi.2009.0098 |
container_title |
OMICS: A Journal of Integrative Biology |
container_volume |
14 |
container_issue |
2 |
container_start_page |
187 |
op_container_end_page |
200 |
_version_ |
1766115379794411520 |