In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique
In vivo rates of protein synthesis in fish have predominantly been measured using a single flooding dose injection of a solution containing 3 H-Phenylalanine as a tracer. However, use of a radiolabelled tracer restricts the application of this technique to controlled laboratory conditions. In this s...
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ftunbrightoncris:oai:pure.atira.dk:publications/77bf92c3-287d-42f1-874f-0b895836a371 2024-09-15T17:56:06+00:00 In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique Owen, S. F. McCarthy, I. D. Watt, P. W. Ladero, V. Sanchez, J. A. Houlihan, D. F. Rennie, M. J. 1999-01-01 https://research.brighton.ac.uk/en/publications/77bf92c3-287d-42f1-874f-0b895836a371 https://doi.org/10.1023/A:1007724012975 http://www.scopus.com/inward/record.url?scp=0003100350&partnerID=8YFLogxK eng eng https://research.brighton.ac.uk/en/publications/77bf92c3-287d-42f1-874f-0b895836a371 info:eu-repo/semantics/restrictedAccess Owen , S F , McCarthy , I D , Watt , P W , Ladero , V , Sanchez , J A , Houlihan , D F & Rennie , M J 1999 , ' In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique ' , Fish Physiology and Biochemistry , vol. 20 , no. 1 , pp. 87-94 . https://doi.org/10.1023/A:1007724012975 N-phenylalanine Growth Mass spectrometry Protein synthesis Stable isotope technology article 1999 ftunbrightoncris https://doi.org/10.1023/A:1007724012975 2024-08-14T23:31:27Z In vivo rates of protein synthesis in fish have predominantly been measured using a single flooding dose injection of a solution containing 3 H-Phenylalanine as a tracer. However, use of a radiolabelled tracer restricts the application of this technique to controlled laboratory conditions. In this study, the flooding dose technique was used to successfully measure in vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts utilising a stable isotope labelled amino acid, L-[ 15 N]-Phenylalanine, as an alternative tracer. Methodologies are presented allowing the 15 N enrichment in the free amino acid pool and in body protein to be measured using mass spectrometry. In this study, the fractional rates of protein consumption (k r ), synthesis (k s ) and growth (k g ) of Atlantic salmon smolts (37 ± 1.4 g) were measured as 4.7, 3.3 and 1.6% day -1 respectively. Protein synthesis retention efficiency (k g k s ) and protein growth efficiency (k g k r )were calculated as 48.5 and 34.0% respectively. The k s , k g k s and k g k r values obtained in this study were found to be within the range of values available in the literature for juvenile salmonid fish of a comparable size. The use of L-[ 15 N]-Phenylalanine provides an alternative tracer to radiolabelled amino acids for measuring in vivo rates of protein synthesis in fish using the flooding dose' technique and also allows studies of fish protein metabolism to be carried out in environmentally sensitive (field and laboratory) conditions where the use of radiolabels is prohibited. Article in Journal/Newspaper Atlantic salmon Salmo salar The University of Brighton Research Portal |
institution |
Open Polar |
collection |
The University of Brighton Research Portal |
op_collection_id |
ftunbrightoncris |
language |
English |
topic |
N-phenylalanine Growth Mass spectrometry Protein synthesis Stable isotope technology |
spellingShingle |
N-phenylalanine Growth Mass spectrometry Protein synthesis Stable isotope technology Owen, S. F. McCarthy, I. D. Watt, P. W. Ladero, V. Sanchez, J. A. Houlihan, D. F. Rennie, M. J. In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
topic_facet |
N-phenylalanine Growth Mass spectrometry Protein synthesis Stable isotope technology |
description |
In vivo rates of protein synthesis in fish have predominantly been measured using a single flooding dose injection of a solution containing 3 H-Phenylalanine as a tracer. However, use of a radiolabelled tracer restricts the application of this technique to controlled laboratory conditions. In this study, the flooding dose technique was used to successfully measure in vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts utilising a stable isotope labelled amino acid, L-[ 15 N]-Phenylalanine, as an alternative tracer. Methodologies are presented allowing the 15 N enrichment in the free amino acid pool and in body protein to be measured using mass spectrometry. In this study, the fractional rates of protein consumption (k r ), synthesis (k s ) and growth (k g ) of Atlantic salmon smolts (37 ± 1.4 g) were measured as 4.7, 3.3 and 1.6% day -1 respectively. Protein synthesis retention efficiency (k g k s ) and protein growth efficiency (k g k r )were calculated as 48.5 and 34.0% respectively. The k s , k g k s and k g k r values obtained in this study were found to be within the range of values available in the literature for juvenile salmonid fish of a comparable size. The use of L-[ 15 N]-Phenylalanine provides an alternative tracer to radiolabelled amino acids for measuring in vivo rates of protein synthesis in fish using the flooding dose' technique and also allows studies of fish protein metabolism to be carried out in environmentally sensitive (field and laboratory) conditions where the use of radiolabels is prohibited. |
format |
Article in Journal/Newspaper |
author |
Owen, S. F. McCarthy, I. D. Watt, P. W. Ladero, V. Sanchez, J. A. Houlihan, D. F. Rennie, M. J. |
author_facet |
Owen, S. F. McCarthy, I. D. Watt, P. W. Ladero, V. Sanchez, J. A. Houlihan, D. F. Rennie, M. J. |
author_sort |
Owen, S. F. |
title |
In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
title_short |
In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
title_full |
In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
title_fullStr |
In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
title_full_unstemmed |
In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique |
title_sort |
in vivo rates of protein synthesis in atlantic salmon (salmo salar l.) smolts determined using a stable isotope flooding dose technique |
publishDate |
1999 |
url |
https://research.brighton.ac.uk/en/publications/77bf92c3-287d-42f1-874f-0b895836a371 https://doi.org/10.1023/A:1007724012975 http://www.scopus.com/inward/record.url?scp=0003100350&partnerID=8YFLogxK |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Owen , S F , McCarthy , I D , Watt , P W , Ladero , V , Sanchez , J A , Houlihan , D F & Rennie , M J 1999 , ' In vivo rates of protein synthesis in Atlantic salmon (Salmo salar L.) smolts determined using a stable isotope flooding dose technique ' , Fish Physiology and Biochemistry , vol. 20 , no. 1 , pp. 87-94 . https://doi.org/10.1023/A:1007724012975 |
op_relation |
https://research.brighton.ac.uk/en/publications/77bf92c3-287d-42f1-874f-0b895836a371 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1023/A:1007724012975 |
_version_ |
1810432308040171520 |