| Summary: | When the freezing is used as a mean of seminal preservation, the viscosity is one characteristic not very well studied. It is considered that the natural semen’s viscosity is related to the sperm conservation. Therefore, the mean goal was to determine the effect of the viscosity of the seminal conservation medium on the acrosomal reaction capacity, and presence and distribution of membrane carbohydrates of Golden eagle’ spermatozoa, preserved in vitro. We worked with 20 ejaculates samples of adult Golden Eagles, which were diluted in seminal conservation media with modified viscosity, added with ficoll in different percentages (0%,10%,30% and 45%). In each condition, basic sperm parameters were determined; from fluorescence with CTC parameters of “not training”, “training” and “spontaneous acrosomal reaction. From fluorescence with FICT-WGA the presence and distribution of membrane carbohydrates were determined. The evaluations post-thaw showed the percentage of live sperm didn’t show statistical difference between groups (P>0.02), but it showed a tendency to increase as the viscosity was higher. In the CTC evaluation was observed that in the groups with higher viscosity, the percentage of trained sperm tend to increase, while the sperm with spontaneous acrosomal reaction were decreased. Finally, the evaluation with the WGA lectin, it was observed that the percentage of spermatozoa with B pattern increased when the viscosity of the medium was higher, however, the spermatozoa with C pattern, remain in similar percentage in all groups, without showing statistical difference (P>0.5). It is concluded that the 10% ficoll inclusion medium was more efficient for the conservation of Golden Eagle spermatozoa by maintaining the structural integrity of the membrane stable in the different parameters evaluated. However, it is also proven that the presence and distribution of the carbohydrates N-acetyglucosamine and sialic acid are altered by the effect of viscosity in the medium, managing to maintain the percentages ...
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