Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected su...
Published in: | International Journal of Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Umeå universitet, Klinisk bakteriologi
2011
|
Subjects: | |
Online Access: | http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-83832 https://doi.org/10.1155/2011/851946 |
id |
ftumeauniv:oai:DiVA.org:umu-83832 |
---|---|
record_format |
openpolar |
spelling |
ftumeauniv:oai:DiVA.org:umu-83832 2023-10-09T21:54:32+02:00 Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters Broman, T Thelaus, J Andersson, A-C Bäckman, S Wikström, P Larsson, E Granberg, M Karlsson, L Bäck, E Eliasson, H Mattsson, R Sjöstedt, Anders Forsman, M 2011 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-83832 https://doi.org/10.1155/2011/851946 eng eng Umeå universitet, Klinisk bakteriologi Department of CBRN Defence and Security, Swedish Defence Research Agency, Umeå Department of Infectious Diseases, Örebro University Hospital, Örebro National Veterinary Institute, Uppsala International Journal of Microbiology, 1687-918X, 2011, 2011, s. Article ID 851946- http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-83832 doi:10.1155/2011/851946 PMID 20885922 Scopus 2-s2.0-79955772721 info:eu-repo/semantics/openAccess Other Basic Medicine Annan medicinsk grundvetenskap Article in journal info:eu-repo/semantics/article text 2011 ftumeauniv https://doi.org/10.1155/2011/851946 2023-09-22T13:58:53Z Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. Article in Journal/Newspaper Northern Sweden Umeå University: Publications (DiVA) International Journal of Microbiology 2011 1 10 |
institution |
Open Polar |
collection |
Umeå University: Publications (DiVA) |
op_collection_id |
ftumeauniv |
language |
English |
topic |
Other Basic Medicine Annan medicinsk grundvetenskap |
spellingShingle |
Other Basic Medicine Annan medicinsk grundvetenskap Broman, T Thelaus, J Andersson, A-C Bäckman, S Wikström, P Larsson, E Granberg, M Karlsson, L Bäck, E Eliasson, H Mattsson, R Sjöstedt, Anders Forsman, M Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
topic_facet |
Other Basic Medicine Annan medicinsk grundvetenskap |
description |
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia. |
format |
Article in Journal/Newspaper |
author |
Broman, T Thelaus, J Andersson, A-C Bäckman, S Wikström, P Larsson, E Granberg, M Karlsson, L Bäck, E Eliasson, H Mattsson, R Sjöstedt, Anders Forsman, M |
author_facet |
Broman, T Thelaus, J Andersson, A-C Bäckman, S Wikström, P Larsson, E Granberg, M Karlsson, L Bäck, E Eliasson, H Mattsson, R Sjöstedt, Anders Forsman, M |
author_sort |
Broman, T |
title |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_short |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_fullStr |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_full_unstemmed |
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters |
title_sort |
molecular detection of persistent francisella tularensis subspecies holarctica in natural waters |
publisher |
Umeå universitet, Klinisk bakteriologi |
publishDate |
2011 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-83832 https://doi.org/10.1155/2011/851946 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
International Journal of Microbiology, 1687-918X, 2011, 2011, s. Article ID 851946- http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-83832 doi:10.1155/2011/851946 PMID 20885922 Scopus 2-s2.0-79955772721 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1155/2011/851946 |
container_title |
International Journal of Microbiology |
container_volume |
2011 |
container_start_page |
1 |
op_container_end_page |
10 |
_version_ |
1779318132040531968 |