Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy
Brown seaweeds contain a variety of saccharides which have potential industrial uses. The most abundant polysaccharide in brown seaweed is typically alginate, consisting of mannuronic (M) and guluronic acid (G). The ratio of these residues fundamentally determines the physicochemical properties of a...
Published in: | International Journal of Biological Macromolecules |
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Umeå universitet, Kemiska institutionen
2024
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ftumeauniv:oai:DiVA.org:umu-219754 2024-02-11T10:06:10+01:00 Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy Niemi, Calle Takahashi, Junko Gorzsás, András Gentili, Francesco G. 2024 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-219754 https://doi.org/10.1016/j.ijbiomac.2023.127870 eng eng Umeå universitet, Kemiska institutionen Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences, Umeå, Sweden Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå, Sweden International Journal of Biological Macromolecules, 0141-8130, 2024, 254, orcid:0000-0002-2298-8844 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-219754 doi:10.1016/j.ijbiomac.2023.127870 PMID 37967607 ISI:001120565300001 Scopus 2-s2.0-85181751462 info:eu-repo/semantics/openAccess Alginate FTIR GC/MS MG ratio North Atlantic brown seaweed Renewable Bioenergy Research Förnyelsebar bioenergi Organic Chemistry Organisk kemi Article in journal info:eu-repo/semantics/article text 2024 ftumeauniv https://doi.org/10.1016/j.ijbiomac.2023.127870 2024-01-24T23:36:24Z Brown seaweeds contain a variety of saccharides which have potential industrial uses. The most abundant polysaccharide in brown seaweed is typically alginate, consisting of mannuronic (M) and guluronic acid (G). The ratio of these residues fundamentally determines the physicochemical properties of alginate. In the present study, gas chromatography/mass spectrometry (GC/MS) was used to give a detailed breakdown of the monosaccharide species in North Atlantic brown seaweeds. The anthrone method was used for determination of crystalline cellulose. The experimental data was used to calibrate multivariate prediction models for estimation of total carbohydrates, crystalline cellulose, total alginate and alginate M/G ratio directly in dried, brown seaweed using three types of infrared spectroscopy, using relative error (RE) as a measure of predictive accuracy. Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) performed well for the estimation of total alginate (RE = 0.12, R2 = 0.82), and attenuated total reflectance (ATR) showed good prediction of M/G ratio (RE = 0.14, R2 = 0.86). Both DRIFTS, ATR and near infrared (NIR) were unable to predict crystalline cellulose and only DRIFTS performed better in determining total carbohydrates. Multivariate spectral analysis is a promising method for easy and rapid characterization of alginate and M/G ratio in seaweed. Article in Journal/Newspaper North Atlantic Umeå University: Publications (DiVA) International Journal of Biological Macromolecules 254 127870 |
institution |
Open Polar |
collection |
Umeå University: Publications (DiVA) |
op_collection_id |
ftumeauniv |
language |
English |
topic |
Alginate FTIR GC/MS MG ratio North Atlantic brown seaweed Renewable Bioenergy Research Förnyelsebar bioenergi Organic Chemistry Organisk kemi |
spellingShingle |
Alginate FTIR GC/MS MG ratio North Atlantic brown seaweed Renewable Bioenergy Research Förnyelsebar bioenergi Organic Chemistry Organisk kemi Niemi, Calle Takahashi, Junko Gorzsás, András Gentili, Francesco G. Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
topic_facet |
Alginate FTIR GC/MS MG ratio North Atlantic brown seaweed Renewable Bioenergy Research Förnyelsebar bioenergi Organic Chemistry Organisk kemi |
description |
Brown seaweeds contain a variety of saccharides which have potential industrial uses. The most abundant polysaccharide in brown seaweed is typically alginate, consisting of mannuronic (M) and guluronic acid (G). The ratio of these residues fundamentally determines the physicochemical properties of alginate. In the present study, gas chromatography/mass spectrometry (GC/MS) was used to give a detailed breakdown of the monosaccharide species in North Atlantic brown seaweeds. The anthrone method was used for determination of crystalline cellulose. The experimental data was used to calibrate multivariate prediction models for estimation of total carbohydrates, crystalline cellulose, total alginate and alginate M/G ratio directly in dried, brown seaweed using three types of infrared spectroscopy, using relative error (RE) as a measure of predictive accuracy. Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) performed well for the estimation of total alginate (RE = 0.12, R2 = 0.82), and attenuated total reflectance (ATR) showed good prediction of M/G ratio (RE = 0.14, R2 = 0.86). Both DRIFTS, ATR and near infrared (NIR) were unable to predict crystalline cellulose and only DRIFTS performed better in determining total carbohydrates. Multivariate spectral analysis is a promising method for easy and rapid characterization of alginate and M/G ratio in seaweed. |
format |
Article in Journal/Newspaper |
author |
Niemi, Calle Takahashi, Junko Gorzsás, András Gentili, Francesco G. |
author_facet |
Niemi, Calle Takahashi, Junko Gorzsás, András Gentili, Francesco G. |
author_sort |
Niemi, Calle |
title |
Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
title_short |
Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
title_full |
Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
title_fullStr |
Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
title_full_unstemmed |
Quantitative and qualitative saccharide analysis of North Atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
title_sort |
quantitative and qualitative saccharide analysis of north atlantic brown seaweed by gas chromatography/mass spectrometry and infrared spectroscopy |
publisher |
Umeå universitet, Kemiska institutionen |
publishDate |
2024 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-219754 https://doi.org/10.1016/j.ijbiomac.2023.127870 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
International Journal of Biological Macromolecules, 0141-8130, 2024, 254, orcid:0000-0002-2298-8844 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-219754 doi:10.1016/j.ijbiomac.2023.127870 PMID 37967607 ISI:001120565300001 Scopus 2-s2.0-85181751462 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.ijbiomac.2023.127870 |
container_title |
International Journal of Biological Macromolecules |
container_volume |
254 |
container_start_page |
127870 |
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1790603698188058624 |