Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters
Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ability to...
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Umeå universitet, Institutionen för ekologi, miljö och geovetenskap
2023
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ftumeauniv:oai:DiVA.org:umu-212480 2024-02-04T10:03:18+01:00 Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann-Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert 2023 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212480 https://doi.org/10.1002/lno.12406 eng eng Umeå universitet, Institutionen för ekologi, miljö och geovetenskap Department of Physical Geography and Ecosystem Science, Lund University, Lund, Sweden Department of Physical Geography and Ecosystem Science, Lund University, Lund, Sweden; School of Geographic Sciences, Nantong University, Nantong, China Department of Earth Sciences, Vrije Universiteit, Amsterdam, Netherlands Limnology and Oceanography, 0024-3590, 2023, 68:9, s. 2059-2069 orcid:0000-0002-5758-2705 orcid:0000-0001-5102-4289 orcid:0000-0001-5730-0694 orcid:0000-0002-2858-6299 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212480 doi:10.1002/lno.12406 ISI:001031950300001 Scopus 2-s2.0-85165443438 info:eu-repo/semantics/openAccess Physical Geography Naturgeografi Article in journal info:eu-repo/semantics/article text 2023 ftumeauniv https://doi.org/10.1002/lno.12406 2024-01-10T23:36:28Z Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ability to predict bacterial production (BP), bacterial respiration (BR), and bacterial growth efficiency (BGE) vary widely, potentially because inorganic nutrient limitation decouples microbial processes from their dependence on DOC composition. We used 28-d bioassays with water from 19 lakes, streams, and rivers in northern Sweden to test how much the links between bacterial metabolism and fluorescence PARAFAC components depend on experimental additions of inorganic nutrients. We found a significant interaction effect between nutrient addition and fluorescence on carbon-specific BP, and weak evidence for influence on BGE by the same interaction (p = 0.1), but no corresponding interaction effect on BR. A practical implication of this interaction was that fluorescence components could explain more than twice as much of the variability in carbon-specific BP (R2 = 0.90) and BGE (R2 = 0.70) after nitrogen and phosphorus addition, compared with control incubations. Our results suggest that an increased supply of labile DOC relative to ambient phosphorus and nitrogen induces gradually larger degrees of nutrient limitation of BP, which in turn decouple BP and BGE from fluorescence signals. Thus, while fluorescence does contain precise information about the degree to which DOC can support microbial processes, this information may be hidden in field studies due to nutrient limitation of bacterial metabolism. Article in Journal/Newspaper Northern Sweden Umeå University: Publications (DiVA) Limnology and Oceanography |
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Umeå University: Publications (DiVA) |
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ftumeauniv |
language |
English |
topic |
Physical Geography Naturgeografi |
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Physical Geography Naturgeografi Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann-Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
topic_facet |
Physical Geography Naturgeografi |
description |
Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ability to predict bacterial production (BP), bacterial respiration (BR), and bacterial growth efficiency (BGE) vary widely, potentially because inorganic nutrient limitation decouples microbial processes from their dependence on DOC composition. We used 28-d bioassays with water from 19 lakes, streams, and rivers in northern Sweden to test how much the links between bacterial metabolism and fluorescence PARAFAC components depend on experimental additions of inorganic nutrients. We found a significant interaction effect between nutrient addition and fluorescence on carbon-specific BP, and weak evidence for influence on BGE by the same interaction (p = 0.1), but no corresponding interaction effect on BR. A practical implication of this interaction was that fluorescence components could explain more than twice as much of the variability in carbon-specific BP (R2 = 0.90) and BGE (R2 = 0.70) after nitrogen and phosphorus addition, compared with control incubations. Our results suggest that an increased supply of labile DOC relative to ambient phosphorus and nitrogen induces gradually larger degrees of nutrient limitation of BP, which in turn decouple BP and BGE from fluorescence signals. Thus, while fluorescence does contain precise information about the degree to which DOC can support microbial processes, this information may be hidden in field studies due to nutrient limitation of bacterial metabolism. |
format |
Article in Journal/Newspaper |
author |
Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann-Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert |
author_facet |
Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann-Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert |
author_sort |
Berggren, Martin |
title |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_short |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_full |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_fullStr |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_full_unstemmed |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_sort |
nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
publisher |
Umeå universitet, Institutionen för ekologi, miljö och geovetenskap |
publishDate |
2023 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212480 https://doi.org/10.1002/lno.12406 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
Limnology and Oceanography, 0024-3590, 2023, 68:9, s. 2059-2069 orcid:0000-0002-5758-2705 orcid:0000-0001-5102-4289 orcid:0000-0001-5730-0694 orcid:0000-0002-2858-6299 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212480 doi:10.1002/lno.12406 ISI:001031950300001 Scopus 2-s2.0-85165443438 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/lno.12406 |
container_title |
Limnology and Oceanography |
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1789970613348073472 |