Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1.
One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated...
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Umeå universitet, Institutionen för ekologi, miljö och geovetenskap
2023
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ftumeauniv:oai:DiVA.org:umu-212218 2024-02-11T09:56:30+01:00 Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita 2023 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212218 https://doi.org/10.1039/d3ra02536e eng eng Umeå universitet, Institutionen för ekologi, miljö och geovetenskap Chemistry Interdisciplinary Project, School of Science and Technology, Chemistry Division, University of Camerino, Camerino, Italy; IridES s.r.l., Camerino, Italy Chemistry Interdisciplinary Project, School of Science and Technology, Chemistry Division, University of Camerino, Camerino, Italy School of Biosciences and Veterinary Medicine, Biosciences and Biotechnology Division, University of Camerino, Camerino, Italy School of Biosciences and Veterinary Medicine, Biosciences and Biotechnology Division, University of Camerino, Camerino, Italy; IridES s.r.l., Camerino, Italy RSC Advances, 2023, 13:28, s. 19276-19285 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212218 doi:10.1039/d3ra02536e PMID 37377865 ISI:001016048500001 Scopus 2-s2.0-85164590678 info:eu-repo/semantics/openAccess Environmental Sciences Miljövetenskap Article in journal info:eu-repo/semantics/article text 2023 ftumeauniv https://doi.org/10.1039/d3ra02536e 2024-01-24T23:36:26Z One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. Its potential in the degradation of hydrocarbons commonly present in diesel oil were studied. The bacterial growth was evaluated in culturing conditions that resembled the marine environment with 1% (v/v) of either diesel or biodiesel added; in both cases, Marinomonas sp. ef1 was able to grow. The chemical oxygen demand measured after the incubation of bacteria with diesel decreased, demonstrating the ability of bacteria to use diesel hydrocarbons as a carbon source and degrade them. The metabolic potential of Marinomonas to degrade aromatic compounds was supported by the identification in the genome of sequences encoding various enzymes involved in benzene and naphthalene degradation. Moreover, in the presence of biodiesel, a fluorescent yellow pigment was produced; this was isolated, purified and characterized by UV-vis and fluorescence spectroscopy, leading to its identification as a pyoverdine. These results suggest that Marinomonas sp. ef1 can be used in hydrocarbon bioremediation and in the transformation of these pollutants in molecules of interest. Article in Journal/Newspaper Antarc* Antarctic Umeå University: Publications (DiVA) Antarctic The Antarctic RSC Advances 13 28 19276 19285 |
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Umeå University: Publications (DiVA) |
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English |
topic |
Environmental Sciences Miljövetenskap |
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Environmental Sciences Miljövetenskap Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
topic_facet |
Environmental Sciences Miljövetenskap |
description |
One of the most concerning environmental problems is represented by petroleum and its derivatives causing contamination of aquatic and underground environments. In this work, the degradation treatment of diesel using Antarctic bacteria is proposed. Marinomonas sp. ef1 is a bacterial strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. Its potential in the degradation of hydrocarbons commonly present in diesel oil were studied. The bacterial growth was evaluated in culturing conditions that resembled the marine environment with 1% (v/v) of either diesel or biodiesel added; in both cases, Marinomonas sp. ef1 was able to grow. The chemical oxygen demand measured after the incubation of bacteria with diesel decreased, demonstrating the ability of bacteria to use diesel hydrocarbons as a carbon source and degrade them. The metabolic potential of Marinomonas to degrade aromatic compounds was supported by the identification in the genome of sequences encoding various enzymes involved in benzene and naphthalene degradation. Moreover, in the presence of biodiesel, a fluorescent yellow pigment was produced; this was isolated, purified and characterized by UV-vis and fluorescence spectroscopy, leading to its identification as a pyoverdine. These results suggest that Marinomonas sp. ef1 can be used in hydrocarbon bioremediation and in the transformation of these pollutants in molecules of interest. |
format |
Article in Journal/Newspaper |
author |
Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita |
author_facet |
Zannotti, Marco Ramasamy, Kesava Priyan Loggi, Valentina Vassallo, Alberto Pucciarelli, Sandra Giovannetti, Rita |
author_sort |
Zannotti, Marco |
title |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_short |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_full |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_fullStr |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_full_unstemmed |
Hydrocarbon degradation strategy and pyoverdine production using the salt tolerant Antarctic bacterium Marinomonas sp. ef1. |
title_sort |
hydrocarbon degradation strategy and pyoverdine production using the salt tolerant antarctic bacterium marinomonas sp. ef1. |
publisher |
Umeå universitet, Institutionen för ekologi, miljö och geovetenskap |
publishDate |
2023 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212218 https://doi.org/10.1039/d3ra02536e |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
RSC Advances, 2023, 13:28, s. 19276-19285 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-212218 doi:10.1039/d3ra02536e PMID 37377865 ISI:001016048500001 Scopus 2-s2.0-85164590678 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1039/d3ra02536e |
container_title |
RSC Advances |
container_volume |
13 |
container_issue |
28 |
container_start_page |
19276 |
op_container_end_page |
19285 |
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1790603680304594944 |