Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden
There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims...
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Umeå universitet, Institutionen för fysiologisk botanik
2021
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ftumeauniv:oai:DiVA.org:umu-183531 2023-10-09T21:54:30+02:00 Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden Nzayisenga, Jean Claude Sellstedt, Anita 2021 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183531 https://doi.org/10.3390/molecules26092410 eng eng Umeå universitet, Institutionen för fysiologisk botanik Umeå universitet, Umeå Plant Science Centre (UPSC) Molecules, 1431-5157, 2021, 26:9, http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183531 doi:10.3390/molecules26092410 ISI:000650660600001 Scopus 2-s2.0-85105239440 info:eu-repo/semantics/openAccess autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Microbiology Mikrobiologi Article in journal info:eu-repo/semantics/article text 2021 ftumeauniv https://doi.org/10.3390/molecules26092410 2023-09-22T14:01:37Z There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia, the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga's lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health. Article in Journal/Newspaper Northern Sweden Umeå University: Publications (DiVA) Molecules 26 9 2410 |
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Umeå University: Publications (DiVA) |
op_collection_id |
ftumeauniv |
language |
English |
topic |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Microbiology Mikrobiologi |
spellingShingle |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Microbiology Mikrobiologi Nzayisenga, Jean Claude Sellstedt, Anita Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
topic_facet |
autotrophy biodiesel Chlorella sp fatty acids heterotrophy metabolomics Microbiology Mikrobiologi |
description |
There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia, the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga's lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health. |
format |
Article in Journal/Newspaper |
author |
Nzayisenga, Jean Claude Sellstedt, Anita |
author_facet |
Nzayisenga, Jean Claude Sellstedt, Anita |
author_sort |
Nzayisenga, Jean Claude |
title |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_short |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_full |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_fullStr |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_full_unstemmed |
Metabolomic Study of Heterotrophically Grown Chlorella sp. Isolated from Wastewater in Northern Sweden |
title_sort |
metabolomic study of heterotrophically grown chlorella sp. isolated from wastewater in northern sweden |
publisher |
Umeå universitet, Institutionen för fysiologisk botanik |
publishDate |
2021 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183531 https://doi.org/10.3390/molecules26092410 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
Molecules, 1431-5157, 2021, 26:9, http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-183531 doi:10.3390/molecules26092410 ISI:000650660600001 Scopus 2-s2.0-85105239440 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/molecules26092410 |
container_title |
Molecules |
container_volume |
26 |
container_issue |
9 |
container_start_page |
2410 |
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1779318086362464256 |