Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human se...
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Umeå universitet, Virologi
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ftumeauniv:oai:DiVA.org:umu-157213 2024-02-11T10:07:11+01:00 Urea dilution of serum for reproducible anti-HSV1 IgG avidity index Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik 2019 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-157213 https://doi.org/10.1186/s12879-019-3769-x eng eng Umeå universitet, Virologi Umeå universitet, Geriatrik Laboratory Medicine, Clinical Microbiology, Umeå University Hospital, Umeå, Sweden BMC Infectious Diseases, 2019, 19, orcid:0000-0001-9094-319X orcid:0000-0002-5271-4780 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-157213 doi:10.1186/s12879-019-3769-x PMID 30764767 ISI:000459030400003 Scopus 2-s2.0-85061523897 info:eu-repo/semantics/openAccess Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin Article in journal info:eu-repo/semantics/article text 2019 ftumeauniv https://doi.org/10.1186/s12879-019-3769-x 2024-01-17T23:36:34Z Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. Article in Journal/Newspaper Northern Sweden Umeå University: Publications (DiVA) BMC Infectious Diseases 19 1 |
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Umeå University: Publications (DiVA) |
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ftumeauniv |
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English |
topic |
Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin |
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Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
topic_facet |
Herpes simplex IgG Avidity ELISA Primary infection Reactivated infection Infectious Medicine Infektionsmedicin |
description |
Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900-0.979) and 0.989 (95% CI 0.969-0.996) in the biobank and clinical samples, respectively. The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. |
format |
Article in Journal/Newspaper |
author |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik |
author_facet |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik |
author_sort |
Olsson, Jan |
title |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_short |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_fullStr |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full_unstemmed |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_sort |
urea dilution of serum for reproducible anti-hsv1 igg avidity index |
publisher |
Umeå universitet, Virologi |
publishDate |
2019 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-157213 https://doi.org/10.1186/s12879-019-3769-x |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
BMC Infectious Diseases, 2019, 19, orcid:0000-0001-9094-319X orcid:0000-0002-5271-4780 http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-157213 doi:10.1186/s12879-019-3769-x PMID 30764767 ISI:000459030400003 Scopus 2-s2.0-85061523897 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12879-019-3769-x |
container_title |
BMC Infectious Diseases |
container_volume |
19 |
container_issue |
1 |
_version_ |
1790605337167921152 |