A song of ice and mud : Interactions of microbes with roots, fauna and carbon in warming permafrost-affected soils

• Main Author: Monteux, Sylvain
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: Umeå universitet, Institutionen för ekologi, miljö och geovetenskap 2018
• Subjects: microbial communities; permafrost; functional limitations; rhizosphere; SOM decomposition; soil fauna; climate change; carbon dioxide; Ecology; Ekologi; Environmental Sciences; Miljövetenskap; Climate Research; Klimatforskning; Microbiology; Mikrobiologi; Geochemistry; Geokemi; Kol’; Sagan; Ice; palsa; Tundra
• Online Access: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-151472

Permafrost-affected soils store a large quantity of soil organic matter (SOM) – ca. half of worldwide soil carbon – and currently undergo rapid and severe warming due to climate change. Increased SOM decomposition by microorganisms and soil fauna due to climate change, poses the risk of a positive climate feedback through the release of greenhouse gases. Direct effects of climate change on SOM decomposition, through such mechanisms as deepening of the seasonally-thawing active layer and increasing soil temperatures, have gathered considerable scientific attention in the last two decades. Yet, indirect effects mediated by changes in plant, microbial, and fauna communities, remain poorly understood. Microbial communities, which may be affected by climate change-induced changes in vegetation composition or rooting patterns, and may in turn affect SOM decomposition, are the primary focus of the work described in this thesis. We used (I) a field-scale permafrost thaw experiment in a palsa peatland, (II) a laboratory incubation of Yedoma permafrost with inoculation by exotic microorganisms, (III) a microcosm experiment with five plant species grown either in Sphagnum peat or in newly-thawed permafrost peat, and (IV) a field-scale cold season warming experiment in cryoturbated tundra to address the indirect effects of climate change on microbial drivers of SOM decomposition. Community composition data for bacteria and fungi were obtained by amplicon sequencing and phospholipid fatty acid extraction, and for collembola by Tullgren extraction, alongside measurements of soil chemistry, CO2 emissions and root density. We showed that in situ thawing of a palsa peatland caused colonization of permafrost soil by overlying soil microbes. Further, we observed that functional limitations of permafrost microbial communities can hamper microbial metabolism in vitro. Relieving these functional limitations in vitro increased cumulative CO2 emissions by 32% over 161 days and introduced nitrification. In addition, we found that ...