Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis
Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti-P. gingivalis antibody levels...
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Umeå universitet, Reumatologi
2016
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ftumeauniv:oai:DiVA.org:umu-126496 2024-09-15T18:26:13+00:00 Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin 2016 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-126496 https://doi.org/10.1186/s13075-016-1100-4 eng eng UmeÃ¥ universitet, Reumatologi Arthritis Research & Therapy , 2016, 18, http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-126496 doi:10.1186/s13075-016-1100-4 PMID 27605245 ISI:000383162300002 Scopus 2-s2.0-84992092229 info:eu-repo/semantics/openAccess Rheumatology and Autoimmunity Reumatologi och inflammation Article in journal info:eu-repo/semantics/article text 2016 ftumeauniv https://doi.org/10.1186/s13075-016-1100-4 2024-07-09T23:37:52Z Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti-P. gingivalis antibody levels are elevated in RA patients. The aim of this study was to investigate whether anti-P. gingivalis antibodies pre-date symptom onset and ACPA production. Methods: A case-control study (251 cases, 198 controls) was performed within the Biobank of Northern Sweden. Cases had donated blood samples (n = 422) before the onset of RA symptoms by 5.2 (6.2) years (median (interquartile range)). Blood was also collected from 192 RA patients following diagnosis. Antibodies against P. gingivalis virulence factor arginine gingipainB (RgpB), and a citrullinated peptide (CPP3) derived from the P. gingivalis peptidylarginine deiminase enzyme, were analysed by ELISA. Results: Anti-RgpB IgG levels were significantly increased in pre-symptomatic individuals (mean +/- SEM; 152.7 +/- 14.8 AU/ml) and in RA patients (114.4 +/- 16.9 AU/ml), compared with controls (p < 0.001). Anti-CPP3 antibodies were detected in 5 % of pre-symptomatic individuals and in 8 % of RA patients, with elevated levels in both subsets (4.33 +/- 0.59 and 9.29 +/- 1.81 AU/ml, respectively) compared with controls (p < 0.001). Anti-CPP3 antibodies followed the ACPA response, with increasing concentrations over time, whilst anti-RgpB antibodies were elevated and stable in the pre-symptomatic individuals with a trend towards lower levels after RA diagnosis. Conclusions: Anti-P. gingivalis antibody concentrations were significantly increased in RA patients compared with controls, and were detectable years before onset of symptoms of RA, supporting an aetiological role for P. gingivalis in the development of RA. Article in Journal/Newspaper Northern Sweden Umeå University: Publications (DiVA) Arthritis Research & Therapy 18 1 |
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Open Polar |
collection |
Umeå University: Publications (DiVA) |
op_collection_id |
ftumeauniv |
language |
English |
topic |
Rheumatology and Autoimmunity Reumatologi och inflammation |
spellingShingle |
Rheumatology and Autoimmunity Reumatologi och inflammation Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
topic_facet |
Rheumatology and Autoimmunity Reumatologi och inflammation |
description |
Background: The periodontal pathogen Porphyromonas gingivalis is hypothesized to be important in rheumatoid arthritis (RA) aetiology by inducing production of anti-citrullinated protein antibodies (ACPA). We have shown that ACPA precede RA onset by years, and that anti-P. gingivalis antibody levels are elevated in RA patients. The aim of this study was to investigate whether anti-P. gingivalis antibodies pre-date symptom onset and ACPA production. Methods: A case-control study (251 cases, 198 controls) was performed within the Biobank of Northern Sweden. Cases had donated blood samples (n = 422) before the onset of RA symptoms by 5.2 (6.2) years (median (interquartile range)). Blood was also collected from 192 RA patients following diagnosis. Antibodies against P. gingivalis virulence factor arginine gingipainB (RgpB), and a citrullinated peptide (CPP3) derived from the P. gingivalis peptidylarginine deiminase enzyme, were analysed by ELISA. Results: Anti-RgpB IgG levels were significantly increased in pre-symptomatic individuals (mean +/- SEM; 152.7 +/- 14.8 AU/ml) and in RA patients (114.4 +/- 16.9 AU/ml), compared with controls (p < 0.001). Anti-CPP3 antibodies were detected in 5 % of pre-symptomatic individuals and in 8 % of RA patients, with elevated levels in both subsets (4.33 +/- 0.59 and 9.29 +/- 1.81 AU/ml, respectively) compared with controls (p < 0.001). Anti-CPP3 antibodies followed the ACPA response, with increasing concentrations over time, whilst anti-RgpB antibodies were elevated and stable in the pre-symptomatic individuals with a trend towards lower levels after RA diagnosis. Conclusions: Anti-P. gingivalis antibody concentrations were significantly increased in RA patients compared with controls, and were detectable years before onset of symptoms of RA, supporting an aetiological role for P. gingivalis in the development of RA. |
format |
Article in Journal/Newspaper |
author |
Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin |
author_facet |
Johansson, Linda Sherina, Natalia Kharlamova, Nastya Potempa, Barbara Larsson, Barbro Israelsson, Lena Potempa, Jan Rantapää-Dahlqvist, Solbritt Lundberg, Karin |
author_sort |
Johansson, Linda |
title |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_short |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_full |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_fullStr |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_full_unstemmed |
Concentration of antibodies against Porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
title_sort |
concentration of antibodies against porphyromonas gingivalis is increased before the onset of symptoms of rheumatoid arthritis |
publisher |
Umeå universitet, Reumatologi |
publishDate |
2016 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-126496 https://doi.org/10.1186/s13075-016-1100-4 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
Arthritis Research & Therapy , 2016, 18, http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-126496 doi:10.1186/s13075-016-1100-4 PMID 27605245 ISI:000383162300002 Scopus 2-s2.0-84992092229 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s13075-016-1100-4 |
container_title |
Arthritis Research & Therapy |
container_volume |
18 |
container_issue |
1 |
_version_ |
1810466661900222464 |