Gas chromatographic-mass spectrometric analysis of polycyclic aromatic hydrocarbon metabolites in Antarctic fish ( Notothenia gibberifrons ) injected with Diesel Fuel Arctic

• Published in: Archives of Environmental Contamination and Toxicology
• Main Authors: Wade, T. L., Fang, J., Yu, Y., Brooks, J. M., McDonald, S.
• Other Authors: National Center for Integrated Bioremediation Research and Development, Department of Civil and Environmental Engineering, The University of Michigan, 48107, Ann Arbor, Michigan, USA, Geochemical and Environmental Research Group, Texas A&M University, 833 Graham Road, 77845, College Station, Texas, USA, Cincinnati Water Works, 5651 Kellogg Avenue, 45228, Cincinnati, Ohio, USA, Ann Arbor
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer-Verlag; Springer-Verlag New York Inc. 1995
• Subjects: Soil Science & Conservation; Environment; General; Ecology; Forestry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Waste Management/Waste Technology; Agriculture; Terrestrial Pollution; Public Health; Health Sciences; Arctic; Antarctic; Antarc*
• Online Access: https://hdl.handle.net/2027.42/48081; https://doi.org/10.1007/BF00212975

Fish (Notothenia gibberifrons) collected in pristine Antarctic sites were injected with Diesel Fuel Arctic (DFA). Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify polycyclic aromatic hydrocarbon (PAH) metabolites obtained from hydrolyzed fish bile. The concentrations of naphthols (NPH), phenanthrenols (PHN), dibenzothiophenols (DBT), and total PAH metabolites (∑PAH) vary with time. The response curve (production of PAH metabolites vs. time) resembled a Sigmoid curve, with an initial low response at 24 h followed by a rapid rise in production of PAH metabolites (55.74 μg/g) 120 h after exposure to DFA. PAH metabolites identified include NPH, PHN, and DBT and their alkylated derivatives, reflecting the composition of the DFA to which the fish were exposed. The GC/MS technique is highly sensitive, particularly in the detection of multi-ring PAH metabolites. The results suggest that analysis of PAH metabolites is a valuable tool for environmental monitoring and assessment of exposure to petroleum. Peer Reviewed http://deepblue.lib.umich.edu/bitstream/2027.42/48081/1/244_2004_Article_BF00212975.pdf