The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)

Melano‐macrophage aggregates, collections of specialized cells of the innate immune system of fish, are considered a general biomarker for contaminant toxicity. To elucidate further the relationship between macrophage aggregates and metals exposure, yelloweye rockfish (Sebastes ruberrimus), a long‐l...

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Published in:Environmental Toxicology and Chemistry
Main Authors: Barst, Benjamin D., Bridges, Kristin, Korbas, Malgorzata, Roberts, Aaron P., Van Kirk, Kray, McNeel, Kevin, Drevnick, Paul E.
Format: Article in Journal/Newspaper
Language:unknown
Published: Lewis Publishers 2015
Subjects:
Mercury
LA‐ICP‐MS
Melano‐macrophage aggregates
Metals
X‐ray fluorescence imaging
Biological Chemistry
Natural Resources and Environment
Science
Prince of Wales Island
Alaska
Online Access:https://hdl.handle.net/2027.42/112257
https://doi.org/10.1002/etc.3009
id ftumdeepblue:oai:deepblue.lib.umich.edu:2027.42/112257
record_format openpolar
institution Open Polar
collection University of Michigan: Deep Blue
op_collection_id ftumdeepblue
language unknown
topic Mercury
LA‐ICP‐MS
Melano‐macrophage aggregates
Metals
X‐ray fluorescence imaging
Biological Chemistry
Natural Resources and Environment
Science
spellingShingle Mercury
LA‐ICP‐MS
Melano‐macrophage aggregates
Metals
X‐ray fluorescence imaging
Biological Chemistry
Natural Resources and Environment
Science
Barst, Benjamin D.
Bridges, Kristin
Korbas, Malgorzata
Roberts, Aaron P.
Van Kirk, Kray
McNeel, Kevin
Drevnick, Paul E.
The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
topic_facet Mercury
LA‐ICP‐MS
Melano‐macrophage aggregates
Metals
X‐ray fluorescence imaging
Biological Chemistry
Natural Resources and Environment
Science
description Melano‐macrophage aggregates, collections of specialized cells of the innate immune system of fish, are considered a general biomarker for contaminant toxicity. To elucidate further the relationship between macrophage aggregates and metals exposure, yelloweye rockfish (Sebastes ruberrimus), a long‐lived species, were sampled from the east and west coasts of Prince of Wales Island, Alaska. Metals concentrations in livers (inorganic Hg, methyl mercury, Se, Ni, Cd, Cu, Zn) and spleens (inorganic Hg and methyl mercury) were determined, as well as their correlations with melano‐macrophage aggregate area. Sections of liver tissue were analyzed by laser ablation‐inductively coupled plasma–mass spectrometry to determine how metals were spatially distributed between hepatocytes and macrophage aggregates. The concentration of inorganic Hg in whole tissue was the best predictor of macrophage area in yelloweye livers and spleens. Macrophage aggregates had higher relative concentrations than most metals compared with the surrounding hepatocytes. However, not all metals were accumulated to the same degree, as evidenced by differences in the ratios of metals in macrophages compared with hepatocytes. Laser ablation data were corroborated with the results of X‐ray synchrotron fluorescence imaging of a yelloweye liver section. Hepatic macrophage aggregates in yelloweye rockfish may play an important role in the detoxification and storage of Hg and other metals. Environ Toxicol Chem 2015;34:1918–1925. © 2015 SETAC Peer Reviewed http://deepblue.lib.umich.edu/bitstream/2027.42/112257/1/etc3009.pdf
format Article in Journal/Newspaper
author Barst, Benjamin D.
Bridges, Kristin
Korbas, Malgorzata
Roberts, Aaron P.
Van Kirk, Kray
McNeel, Kevin
Drevnick, Paul E.
author_facet Barst, Benjamin D.
Bridges, Kristin
Korbas, Malgorzata
Roberts, Aaron P.
Van Kirk, Kray
McNeel, Kevin
Drevnick, Paul E.
author_sort Barst, Benjamin D.
title The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
title_short The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
title_full The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
title_fullStr The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
title_full_unstemmed The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)
title_sort role of melano‐macrophage aggregates in the storage of mercury and other metals: an example from yelloweye rockfish (sebastes ruberrimus)
publisher Lewis Publishers
publishDate 2015
url https://hdl.handle.net/2027.42/112257
https://doi.org/10.1002/etc.3009
long_lat ENVELOPE(-99.001,-99.001,72.668,72.668)
geographic Prince of Wales Island
geographic_facet Prince of Wales Island
genre Prince of Wales Island
Alaska
genre_facet Prince of Wales Island
Alaska
op_relation Barst, Benjamin D.; Bridges, Kristin; Korbas, Malgorzata; Roberts, Aaron P.; Van Kirk, Kray; McNeel, Kevin; Drevnick, Paul E. (2015). "The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)." Environmental Toxicology and Chemistry 34(8): 1918-1925.
0730-7268
1552-8618
https://hdl.handle.net/2027.42/112257
doi:10.1002/etc.3009
Environmental Toxicology and Chemistry
Van Dyk J, Cochrane M, Wagenaar G. 2012. Liver histopathology of the sharptooth catfish Clarias gariepinus as a biomarker of aquatic pollution. Chemosphere 87: 301 – 311.
Karimi R, Chen CY, Pickhardt PC, Fisher NS, Folt CL. 2007. Stoichiometric controls of mercury dilution by growth. Proc Natl Acad Sci 104: 7477 – 7482.
Ward DM, Nislow KH, Chen CY, Folt CL. 2010. Rapid, efficient growth reduces mercury concentrations in stream‐dwelling Atlantic salmon. Trans Am Fish Soc 139: 1 – 10.
Rajotte JW, Couture P. 2002. Effects of environmental metal contamination on the condition, swimming performance, and tissue metabolic capacities of wild yellow perch ( Perca flavescens ). Can J Fish Aquat Sci 59: 1296 – 1304.
Dethloff G, Bailey H, Maier K. 2001. Effects of dissolved copper on select hematological, biochemical, and immunological parameters of wild rainbow trout ( Oncorhynchus mykiss ). Arch Environ Contam Toxicol 40: 371 – 380.
Ptashynski M, Pedlar R, Evans R, Baron C, Klaverkamp J. 2002. Toxicology of dietary nickel in lake whitefish ( Coregonus clupeaformis ). Aquat Toxicol 58: 229 – 247.
Drevnick P, Roberts A, Otter R, Hammerschmidt C, Klaper R, Oris J. 2008. Mercury toxicity in livers of northern pike (Esox lucius) from Isle Royale, USA. Comp Biochem Physiol C 147: 331 – 338.
Di Giulio RT, Hinton DE. 2008. The Toxicology of Fishes. CRC, Boca Raton, FL, USA.
Van der Oost R, Beyer J, Vermeulen NP. 2003. Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environ Toxicol Pharmacol 13: 57 – 149.
Pereira J, Mercaldo‐Allen R, Kuropat C, Luedke D, Sennefelder G. 1993. Effect of cadmium accumulation on serum vitellogenin levels and hepatosomatic and gonadosomatic indices of winter flounder ( Pleuronectes americanus ). Arch Environ Contam Toxicol 24: 427 – 431.
Baker R, Handy R, Davies S, Snook J. 1998. Chronic dietary exposure to copper affects growth, tissue lipid peroxidation, and metal composition of the grey mullet, Chelon labrosus. Mar Environ Res 45: 357 – 365.
Larose C, Canuel R, Lucotte M, Di Giulio RT. 2008. Toxicological effects of methylmercury on walleye ( Sander vitreus ) and perch ( Perca flavescens ) from lakes of the boreal forest. Comp Biochem Physiol C 147: 139 – 149.
Giguère A, Campbell PG, Hare L, McDonald DG, Rasmussen JB. 2004. Influence of lake chemistry and fish age on cadmium, copper, and zinc concentrations in various organs of indigenous yellow perch ( Perca flavescens ). Can J Fish Aquat Sci 61: 1702 – 1716.
Berntssen M, Hylland K, Julshamn K, Lundebye AK, Waagbø R. 2004. Maximum limits of organic and inorganic mercury in fish feed. Aquacult Nutr 10: 83 – 97.
Cizdziel J, Hinners T, Cross C, Pollard J. 2003. Distribution of mercury in the tissues of five species of freshwater fish from Lake Mead, USA. J Environ Monit 5: 802 – 807.
Pulsford A, Ryan K, Nott J. 1992. Metals and melanomacrophages in flounder, Platichthys flesus, spleen and kidney. J Mar Biol Assoc UK 72: 483 – 498.
Woshner V, O'Hara T, Eurell J, Wallig M, Bratton G, Suydam R, Beasley V. 2002. Distribution of inorganic mercury in liver and kidney of beluga and bowhead whales through autometallographic development of light microscopic tissue sections. Toxicol Pathol 30: 209.
Bertini I. 2007. Biological Inorganic Chemistry: Structure and Reactivity. University Science Books, Herndon, VA, USA.
Manni ML, Tomai LP, Norris CA, Thomas LM, Kelley EE, Salter RD, Crapo JD, Chang L‐YL, Watkins SC, Piganelli JD. 2011. Extracellular superoxide dismutase in macrophages augments bacterial killing by promoting phagocytosis. Am J Pathol 178: 2752 – 2759.
Babu U, Failla ML. 1990. Respiratory burst and candidacidal activity of peritoneal macrophages are impaired in copper‐deficient rats. J Nutr 120: 1692 – 1699.
Cerone S, Sansinanea A, Streitenberger S, Garcia C, Auza N. 2000. Bovine monocyte‐derived macrophage function in induced copper deficiency. Gen Physiol Biophys 19: 49 – 58.
Rotruck J, Pope A, Ganther H, Swanson A, Hafeman DG, Hoekstra W. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science 179: 588 – 590.
Pařízek J, Ošt′ádalová I. 1967. The protective effect of small amounts of selenite in sublimate intoxication. Experientia 23: 142 – 143.
Ganther H, Goudie C, Sunde M, Kopecky M, Wanger P, Hoh S, Hoekstra W. 1972. Selenium: Relation to decreased toxicity of methylmercury added to diets containing tuna. Science (Wash) 175: 1122 – 1124.
Khan MAK, Wang F. 2009. Mercury selenium compounds and their toxicological significance: Toward a molecular understanding of the mercury selenium antagonism. Environ Toxicol Chem 28: 1567 – 1577.
Ralston NV, Blackwell III JL, Raymond LJ. 2007. Importance of molar ratios in selenium‐dependent protection against methylmercury toxicity. Biol Trace Elem Res 119: 255 – 268.
Martoja R, Berry JP. 1980. Identification of tiemannite as a probable product of demethylation of mercury by selenium in cetaceans: A complement to the scheme of the biological cycle of mercury. Vie Milieu 30: 7 – 10.
Wolke R. 1992. Piscine macrophage aggregates: A review. Annu Rev Fish Dis 2: 91 – 108.
Agius C, Roberts R. 2003. Melano macrophage centres and their role in fish pathology. J Fish Dis 26: 499 – 509.
McCarthy J, Shugart L. 1990. Biomarkers of Environmental Contamination. Lewis Publishers, Chelsea, MI, USA.
Capps T, Mukhi S, Rinchard JJ, Theodorakis CW, Blazer VS, Patiño R. 2004. Exposure to perchlorate induces the formation of macrophage aggregates in the trunk kidney of zebrafish and mosquitofish. J Aquat Anim Health 16: 145 – 151.
Mela M, Randi M, Ventura D, Carvalho C, Pelletier E, Oliveira Ribeiro C. 2007. Effects of dietary methylmercury on liver and kidney histology in the neotropical fish Hoplias malabaricus. Ecotoxicol Environ Saf 68: 426 – 435.
Giari L, Manera M, Simoni E, Dezfuli B. 2007. Cellular alterations in different organs of European sea bass Dicentrarchus labrax (L.) exposed to cadmium. Chemosphere 67: 1171 – 1181.
Khan R. 2003. Health of flatfish from localities in Placentia Bay, Newfoundland, contaminated with petroleum and PCBs. Arch Environ Contam Toxicol 44: 485 – 492.
Raldúa D, Bayona J, Barceló D. 2007. Mercury levels and liver pathology in feral fish living in the vicinity of a mercury cell chlor‐alkali factory. Chemosphere 66: 1217 – 1225.
Schwindt AR, Fournie JW, Landers DH, Schreck CB, Kent ML. 2008. Mercury concentrations in salmonids from western US national parks and relationships with age and macrophage aggregates. Environ Sci Technol 42: 1365 – 1370.
Barst BD, Gevertz AK, Chumchal MM, Smith JD, Rainwater T, Drevnick P, Hudelson KE, Hart A, Verbeck GF, Roberts AP. 2011. Laser ablation ICP‐MS co‐localization of mercury and immune response in fish. Environ Sci Technol 45: 8982 – 8988.
Batchelar KL, Kidd KA, Drevnick PE, Munkittrick KR, Burgess NM, Roberts AP, Smith JD. 2013. Evidence of impaired health in yellow perch (Perca flavescens) from a biological mercury hotspot in northeastern North America. Environ Toxicol Chem 32: 627 – 637.
MacLellan S, Station PB. 1997. How to Age Rockfish (Sebastes) Using S. Alutus as an Example, the Otolith Burnt Section Technique. Fisheries and Oceans Canada, Nanaimo, BC, Canada.
Yamanaka KL, Lacko L, Withler R, Grandin C, Lochead J, Martin J, Olsen N, Wallace S. 2006. A Review of Yelloweye Rockfish Sebastes ruberrimus Along the Pacific Coast of Canada: Biology, Distribution and Abundance Trends. Fisheries and Oceans Canada, Science, Nanaimo, BC, Canada.
Matthews KR. 1990. A telemetric study of the home ranges and homing routes of copper and quillback rockfishes on shallow rocky reefs. Can J Zool 68: 2243 – 2250.
Jorgensen SJ, Kaplan DM, Klimley A, Morgan SG, O'Farrell MR, Botsford LW. 2006. Limited movement in blue rockfish Sebastes mystinus: Internal structure of home range. Mar Ecol Prog Ser 327: 157.
Barst BD, Hammerschmidt CR, Chumchal MM, Muir DC, Smith JD, Roberts AP, Rainwater TR, Drevnick PE. 2013. Determination of mercury speciation in fish tissue with a direct mercury analyzer. Environ Toxicol Chem 32: 1237 – 1241.
Beaudin L, Johannessen SC, Macdonald RW. 2010. Coupling laser ablation and atomic fluorescence spectrophotometry: An example using mercury analysis of small sections of fish scales. Anal Chem 82: 8785 – 8788.
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spelling ftumdeepblue:oai:deepblue.lib.umich.edu:2027.42/112257 2023-08-20T04:09:23+02:00 The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus) Barst, Benjamin D. Bridges, Kristin Korbas, Malgorzata Roberts, Aaron P. Van Kirk, Kray McNeel, Kevin Drevnick, Paul E. 2015-08 application/pdf https://hdl.handle.net/2027.42/112257 https://doi.org/10.1002/etc.3009 unknown Lewis Publishers Wiley Periodicals, Inc. Barst, Benjamin D.; Bridges, Kristin; Korbas, Malgorzata; Roberts, Aaron P.; Van Kirk, Kray; McNeel, Kevin; Drevnick, Paul E. (2015). "The role of melano‐macrophage aggregates in the storage of mercury and other metals: An example from yelloweye rockfish (Sebastes ruberrimus)." Environmental Toxicology and Chemistry 34(8): 1918-1925. 0730-7268 1552-8618 https://hdl.handle.net/2027.42/112257 doi:10.1002/etc.3009 Environmental Toxicology and Chemistry Van Dyk J, Cochrane M, Wagenaar G. 2012. Liver histopathology of the sharptooth catfish Clarias gariepinus as a biomarker of aquatic pollution. Chemosphere 87: 301 – 311. Karimi R, Chen CY, Pickhardt PC, Fisher NS, Folt CL. 2007. Stoichiometric controls of mercury dilution by growth. Proc Natl Acad Sci 104: 7477 – 7482. Ward DM, Nislow KH, Chen CY, Folt CL. 2010. Rapid, efficient growth reduces mercury concentrations in stream‐dwelling Atlantic salmon. Trans Am Fish Soc 139: 1 – 10. Rajotte JW, Couture P. 2002. Effects of environmental metal contamination on the condition, swimming performance, and tissue metabolic capacities of wild yellow perch ( Perca flavescens ). Can J Fish Aquat Sci 59: 1296 – 1304. Dethloff G, Bailey H, Maier K. 2001. Effects of dissolved copper on select hematological, biochemical, and immunological parameters of wild rainbow trout ( Oncorhynchus mykiss ). Arch Environ Contam Toxicol 40: 371 – 380. Ptashynski M, Pedlar R, Evans R, Baron C, Klaverkamp J. 2002. Toxicology of dietary nickel in lake whitefish ( Coregonus clupeaformis ). Aquat Toxicol 58: 229 – 247. Drevnick P, Roberts A, Otter R, Hammerschmidt C, Klaper R, Oris J. 2008. Mercury toxicity in livers of northern pike (Esox lucius) from Isle Royale, USA. Comp Biochem Physiol C 147: 331 – 338. Di Giulio RT, Hinton DE. 2008. The Toxicology of Fishes. CRC, Boca Raton, FL, USA. Van der Oost R, Beyer J, Vermeulen NP. 2003. Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environ Toxicol Pharmacol 13: 57 – 149. Pereira J, Mercaldo‐Allen R, Kuropat C, Luedke D, Sennefelder G. 1993. Effect of cadmium accumulation on serum vitellogenin levels and hepatosomatic and gonadosomatic indices of winter flounder ( Pleuronectes americanus ). Arch Environ Contam Toxicol 24: 427 – 431. Baker R, Handy R, Davies S, Snook J. 1998. Chronic dietary exposure to copper affects growth, tissue lipid peroxidation, and metal composition of the grey mullet, Chelon labrosus. Mar Environ Res 45: 357 – 365. Larose C, Canuel R, Lucotte M, Di Giulio RT. 2008. Toxicological effects of methylmercury on walleye ( Sander vitreus ) and perch ( Perca flavescens ) from lakes of the boreal forest. Comp Biochem Physiol C 147: 139 – 149. Giguère A, Campbell PG, Hare L, McDonald DG, Rasmussen JB. 2004. Influence of lake chemistry and fish age on cadmium, copper, and zinc concentrations in various organs of indigenous yellow perch ( Perca flavescens ). Can J Fish Aquat Sci 61: 1702 – 1716. Berntssen M, Hylland K, Julshamn K, Lundebye AK, Waagbø R. 2004. Maximum limits of organic and inorganic mercury in fish feed. Aquacult Nutr 10: 83 – 97. Cizdziel J, Hinners T, Cross C, Pollard J. 2003. Distribution of mercury in the tissues of five species of freshwater fish from Lake Mead, USA. J Environ Monit 5: 802 – 807. Pulsford A, Ryan K, Nott J. 1992. Metals and melanomacrophages in flounder, Platichthys flesus, spleen and kidney. J Mar Biol Assoc UK 72: 483 – 498. Woshner V, O'Hara T, Eurell J, Wallig M, Bratton G, Suydam R, Beasley V. 2002. Distribution of inorganic mercury in liver and kidney of beluga and bowhead whales through autometallographic development of light microscopic tissue sections. Toxicol Pathol 30: 209. Bertini I. 2007. Biological Inorganic Chemistry: Structure and Reactivity. University Science Books, Herndon, VA, USA. Manni ML, Tomai LP, Norris CA, Thomas LM, Kelley EE, Salter RD, Crapo JD, Chang L‐YL, Watkins SC, Piganelli JD. 2011. Extracellular superoxide dismutase in macrophages augments bacterial killing by promoting phagocytosis. Am J Pathol 178: 2752 – 2759. Babu U, Failla ML. 1990. Respiratory burst and candidacidal activity of peritoneal macrophages are impaired in copper‐deficient rats. J Nutr 120: 1692 – 1699. Cerone S, Sansinanea A, Streitenberger S, Garcia C, Auza N. 2000. Bovine monocyte‐derived macrophage function in induced copper deficiency. Gen Physiol Biophys 19: 49 – 58. Rotruck J, Pope A, Ganther H, Swanson A, Hafeman DG, Hoekstra W. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science 179: 588 – 590. Pařízek J, Ošt′ádalová I. 1967. The protective effect of small amounts of selenite in sublimate intoxication. Experientia 23: 142 – 143. Ganther H, Goudie C, Sunde M, Kopecky M, Wanger P, Hoh S, Hoekstra W. 1972. Selenium: Relation to decreased toxicity of methylmercury added to diets containing tuna. Science (Wash) 175: 1122 – 1124. Khan MAK, Wang F. 2009. Mercury selenium compounds and their toxicological significance: Toward a molecular understanding of the mercury selenium antagonism. Environ Toxicol Chem 28: 1567 – 1577. Ralston NV, Blackwell III JL, Raymond LJ. 2007. Importance of molar ratios in selenium‐dependent protection against methylmercury toxicity. Biol Trace Elem Res 119: 255 – 268. Martoja R, Berry JP. 1980. Identification of tiemannite as a probable product of demethylation of mercury by selenium in cetaceans: A complement to the scheme of the biological cycle of mercury. Vie Milieu 30: 7 – 10. Wolke R. 1992. Piscine macrophage aggregates: A review. Annu Rev Fish Dis 2: 91 – 108. Agius C, Roberts R. 2003. Melano macrophage centres and their role in fish pathology. J Fish Dis 26: 499 – 509. McCarthy J, Shugart L. 1990. Biomarkers of Environmental Contamination. Lewis Publishers, Chelsea, MI, USA. Capps T, Mukhi S, Rinchard JJ, Theodorakis CW, Blazer VS, Patiño R. 2004. Exposure to perchlorate induces the formation of macrophage aggregates in the trunk kidney of zebrafish and mosquitofish. J Aquat Anim Health 16: 145 – 151. Mela M, Randi M, Ventura D, Carvalho C, Pelletier E, Oliveira Ribeiro C. 2007. Effects of dietary methylmercury on liver and kidney histology in the neotropical fish Hoplias malabaricus. Ecotoxicol Environ Saf 68: 426 – 435. Giari L, Manera M, Simoni E, Dezfuli B. 2007. Cellular alterations in different organs of European sea bass Dicentrarchus labrax (L.) exposed to cadmium. Chemosphere 67: 1171 – 1181. Khan R. 2003. Health of flatfish from localities in Placentia Bay, Newfoundland, contaminated with petroleum and PCBs. Arch Environ Contam Toxicol 44: 485 – 492. Raldúa D, Bayona J, Barceló D. 2007. Mercury levels and liver pathology in feral fish living in the vicinity of a mercury cell chlor‐alkali factory. Chemosphere 66: 1217 – 1225. Schwindt AR, Fournie JW, Landers DH, Schreck CB, Kent ML. 2008. Mercury concentrations in salmonids from western US national parks and relationships with age and macrophage aggregates. Environ Sci Technol 42: 1365 – 1370. Barst BD, Gevertz AK, Chumchal MM, Smith JD, Rainwater T, Drevnick P, Hudelson KE, Hart A, Verbeck GF, Roberts AP. 2011. Laser ablation ICP‐MS co‐localization of mercury and immune response in fish. Environ Sci Technol 45: 8982 – 8988. Batchelar KL, Kidd KA, Drevnick PE, Munkittrick KR, Burgess NM, Roberts AP, Smith JD. 2013. Evidence of impaired health in yellow perch (Perca flavescens) from a biological mercury hotspot in northeastern North America. Environ Toxicol Chem 32: 627 – 637. MacLellan S, Station PB. 1997. How to Age Rockfish (Sebastes) Using S. Alutus as an Example, the Otolith Burnt Section Technique. Fisheries and Oceans Canada, Nanaimo, BC, Canada. Yamanaka KL, Lacko L, Withler R, Grandin C, Lochead J, Martin J, Olsen N, Wallace S. 2006. A Review of Yelloweye Rockfish Sebastes ruberrimus Along the Pacific Coast of Canada: Biology, Distribution and Abundance Trends. Fisheries and Oceans Canada, Science, Nanaimo, BC, Canada. Matthews KR. 1990. A telemetric study of the home ranges and homing routes of copper and quillback rockfishes on shallow rocky reefs. Can J Zool 68: 2243 – 2250. Jorgensen SJ, Kaplan DM, Klimley A, Morgan SG, O'Farrell MR, Botsford LW. 2006. Limited movement in blue rockfish Sebastes mystinus: Internal structure of home range. Mar Ecol Prog Ser 327: 157. Barst BD, Hammerschmidt CR, Chumchal MM, Muir DC, Smith JD, Roberts AP, Rainwater TR, Drevnick PE. 2013. Determination of mercury speciation in fish tissue with a direct mercury analyzer. Environ Toxicol Chem 32: 1237 – 1241. Beaudin L, Johannessen SC, Macdonald RW. 2010. Coupling laser ablation and atomic fluorescence spectrophotometry: An example using mercury analysis of small sections of fish scales. Anal Chem 82: 8785 – 8788. IndexNoFollow Mercury LA‐ICP‐MS Melano‐macrophage aggregates Metals X‐ray fluorescence imaging Biological Chemistry Natural Resources and Environment Science Article 2015 ftumdeepblue https://doi.org/10.1002/etc.3009 2023-07-31T21:07:47Z Melano‐macrophage aggregates, collections of specialized cells of the innate immune system of fish, are considered a general biomarker for contaminant toxicity. To elucidate further the relationship between macrophage aggregates and metals exposure, yelloweye rockfish (Sebastes ruberrimus), a long‐lived species, were sampled from the east and west coasts of Prince of Wales Island, Alaska. Metals concentrations in livers (inorganic Hg, methyl mercury, Se, Ni, Cd, Cu, Zn) and spleens (inorganic Hg and methyl mercury) were determined, as well as their correlations with melano‐macrophage aggregate area. Sections of liver tissue were analyzed by laser ablation‐inductively coupled plasma–mass spectrometry to determine how metals were spatially distributed between hepatocytes and macrophage aggregates. The concentration of inorganic Hg in whole tissue was the best predictor of macrophage area in yelloweye livers and spleens. Macrophage aggregates had higher relative concentrations than most metals compared with the surrounding hepatocytes. However, not all metals were accumulated to the same degree, as evidenced by differences in the ratios of metals in macrophages compared with hepatocytes. Laser ablation data were corroborated with the results of X‐ray synchrotron fluorescence imaging of a yelloweye liver section. Hepatic macrophage aggregates in yelloweye rockfish may play an important role in the detoxification and storage of Hg and other metals. Environ Toxicol Chem 2015;34:1918–1925. © 2015 SETAC Peer Reviewed http://deepblue.lib.umich.edu/bitstream/2027.42/112257/1/etc3009.pdf Article in Journal/Newspaper Prince of Wales Island Alaska University of Michigan: Deep Blue Prince of Wales Island ENVELOPE(-99.001,-99.001,72.668,72.668) Environmental Toxicology and Chemistry 34 8 1918 1925

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