Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research
Compounds and products in the biocide and plant protection sector can only be registered after formal risk assessment to ensure safety for users and the environment. In bird and mammal risk assessment, this is routinely done using generic focal species as models, which are of particular exposure ris...
Published in: | Journal of Applied Toxicology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://research.manchester.ac.uk/en/publications/d3b7fc9f-59e0-4c52-a8d1-9e3aa6d543c2 https://doi.org/10.1002/jat.3635 |
id |
ftumanchesterpub:oai:pure.atira.dk:publications/d3b7fc9f-59e0-4c52-a8d1-9e3aa6d543c2 |
---|---|
record_format |
openpolar |
spelling |
ftumanchesterpub:oai:pure.atira.dk:publications/d3b7fc9f-59e0-4c52-a8d1-9e3aa6d543c2 2023-11-12T04:15:59+01:00 Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research Imholt, Christian Abdulla, Tariq Stevens, Alexander Edwards, Peter Jacob, Jens Woods, David Rogers, Elaine Aarons, Leon Segelcke, Daniel 2018-09 https://research.manchester.ac.uk/en/publications/d3b7fc9f-59e0-4c52-a8d1-9e3aa6d543c2 https://doi.org/10.1002/jat.3635 eng eng info:eu-repo/semantics/closedAccess Imholt , C , Abdulla , T , Stevens , A , Edwards , P , Jacob , J , Woods , D , Rogers , E , Aarons , L & Segelcke , D 2018 , ' Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research ' , Journal of Applied Toxicology , vol. 38 , no. 9 . https://doi.org/10.1002/jat.3635 article 2018 ftumanchesterpub https://doi.org/10.1002/jat.3635 2023-10-30T09:16:17Z Compounds and products in the biocide and plant protection sector can only be registered after formal risk assessment to ensure safety for users and the environment. In bird and mammal risk assessment, this is routinely done using generic focal species as models, which are of particular exposure risk. Such a species is the common vole (Microtus arvalis) due to its high food intake relative to the low body weight. For wild species, biological samples, data and hence realistic exposure estimations are particularly difficult to obtain. In recent years, advances have been made in the techniques related to serial microsampling of laboratory mice and rats that allow for a reduction in sampling volumes. Similar progress in wild species sampling is missing. This study presents a proof of concept to dose wild rodents with relevant compounds and to draw serial, low volume blood samples suitable for state‐of‐the art toxicokinetic analyses. For the first time, the jugular vein of common voles was used to administer compounds (two frequently used fungicidal components). This procedure and the following microsampling of blood (2 × 10 μl six times within 24 hours) from the lateral tail vein did not affect body weight and mortality of voles. Samples were sufficient to detect dissipation patterns of the compounds from blood in toxicokinetic analysis. These results suggest that microsampling can be well translated from laboratory mice to wild rodent species and help to obtain realistic exposure estimates in wild rodents for ecotoxicological studies as well as to promote the 3R concept in studies with wild rodent species. Article in Journal/Newspaper Common vole Microtus arvalis The University of Manchester: Research Explorer Journal of Applied Toxicology 38 9 1244 1250 |
institution |
Open Polar |
collection |
The University of Manchester: Research Explorer |
op_collection_id |
ftumanchesterpub |
language |
English |
description |
Compounds and products in the biocide and plant protection sector can only be registered after formal risk assessment to ensure safety for users and the environment. In bird and mammal risk assessment, this is routinely done using generic focal species as models, which are of particular exposure risk. Such a species is the common vole (Microtus arvalis) due to its high food intake relative to the low body weight. For wild species, biological samples, data and hence realistic exposure estimations are particularly difficult to obtain. In recent years, advances have been made in the techniques related to serial microsampling of laboratory mice and rats that allow for a reduction in sampling volumes. Similar progress in wild species sampling is missing. This study presents a proof of concept to dose wild rodents with relevant compounds and to draw serial, low volume blood samples suitable for state‐of‐the art toxicokinetic analyses. For the first time, the jugular vein of common voles was used to administer compounds (two frequently used fungicidal components). This procedure and the following microsampling of blood (2 × 10 μl six times within 24 hours) from the lateral tail vein did not affect body weight and mortality of voles. Samples were sufficient to detect dissipation patterns of the compounds from blood in toxicokinetic analysis. These results suggest that microsampling can be well translated from laboratory mice to wild rodent species and help to obtain realistic exposure estimates in wild rodents for ecotoxicological studies as well as to promote the 3R concept in studies with wild rodent species. |
format |
Article in Journal/Newspaper |
author |
Imholt, Christian Abdulla, Tariq Stevens, Alexander Edwards, Peter Jacob, Jens Woods, David Rogers, Elaine Aarons, Leon Segelcke, Daniel |
spellingShingle |
Imholt, Christian Abdulla, Tariq Stevens, Alexander Edwards, Peter Jacob, Jens Woods, David Rogers, Elaine Aarons, Leon Segelcke, Daniel Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
author_facet |
Imholt, Christian Abdulla, Tariq Stevens, Alexander Edwards, Peter Jacob, Jens Woods, David Rogers, Elaine Aarons, Leon Segelcke, Daniel |
author_sort |
Imholt, Christian |
title |
Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
title_short |
Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
title_full |
Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
title_fullStr |
Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
title_full_unstemmed |
Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
title_sort |
establishment and validation of microsampling techniques in wild rodents for ecotoxicological research |
publishDate |
2018 |
url |
https://research.manchester.ac.uk/en/publications/d3b7fc9f-59e0-4c52-a8d1-9e3aa6d543c2 https://doi.org/10.1002/jat.3635 |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Imholt , C , Abdulla , T , Stevens , A , Edwards , P , Jacob , J , Woods , D , Rogers , E , Aarons , L & Segelcke , D 2018 , ' Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research ' , Journal of Applied Toxicology , vol. 38 , no. 9 . https://doi.org/10.1002/jat.3635 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1002/jat.3635 |
container_title |
Journal of Applied Toxicology |
container_volume |
38 |
container_issue |
9 |
container_start_page |
1244 |
op_container_end_page |
1250 |
_version_ |
1782333197534625792 |