Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog
Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate ge...
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ftumanchesterpub:oai:pure.atira.dk:publications/85c0501e-3801-49b6-a8f9-92064c4129bd 2024-06-23T07:54:42+00:00 Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog Wiersma, Anje C. Stabej, Polona Leegwater, Peter A J Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna 2008-01 https://research.manchester.ac.uk/en/publications/85c0501e-3801-49b6-a8f9-92064c4129bd https://doi.org/10.1093/jhered/esm090 eng eng https://research.manchester.ac.uk/en/publications/85c0501e-3801-49b6-a8f9-92064c4129bd info:eu-repo/semantics/closedAccess Wiersma , A C , Stabej , P , Leegwater , P A J , Van Oost , B A , Ollier , W E & Dukes-McEwan , J 2008 , ' Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog ' , Journal of Heredity , vol. 99 , no. 1 , pp. 73-80 . https://doi.org/10.1093/jhered/esm090 ACADEMIC JOURNAL PAPERS ORIGINAL ARTICLES article 2008 ftumanchesterpub https://doi.org/10.1093/jhered/esm090 2024-06-04T00:23:32Z Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate genes for their involvement in DCM in the Newfoundland dog. Polymorphic microsatellite markers and single Nucleotide Polymorphisms were genotyped in 4 families of Newfoundland dogs segregating dilated cardiomyopathy for the genes encoding α-cardiac actin (ACTC), caveolin (CAVI), cysteine-rich protein 3 (CSRP3), LIM-domain binding factor 3 (LDB3), desmin (DES), lamin A/C (LMNA), myosin heavy polypeptide 7 (MYH7), delta-sarcoglycan (SGCD), troponin I (TNNTI3), troponin T (TNNT2), alpha-tropomyosin (TPMI), titin (TTN) and vinculin (VCL). A Logarithm of the odds (LOD) score of less than -2.0 in 2-point linkage analysis indicated exclusion of all but 2 genes, encoding CSRP3 and DES. A (LOD) score between -1.5 and -2.0 for CSRP3 and DES makes these genes unlikely causes of DCM in this dog breed. For the phospholamban (PLN) and titin cap (TTN) genes, a direct mutation screening approach was used. DNA sequence analysis of all exons showed no evidence that these genes are involved in DCM in the Newfoundland dog. © The American Genetic Association. 2007. All rights reserved. Article in Journal/Newspaper Newfoundland The University of Manchester: Research Explorer Journal of Heredity 99 1 73 80 |
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Open Polar |
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The University of Manchester: Research Explorer |
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ftumanchesterpub |
language |
English |
topic |
ACADEMIC JOURNAL PAPERS ORIGINAL ARTICLES |
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ACADEMIC JOURNAL PAPERS ORIGINAL ARTICLES Wiersma, Anje C. Stabej, Polona Leegwater, Peter A J Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
topic_facet |
ACADEMIC JOURNAL PAPERS ORIGINAL ARTICLES |
description |
Dilated cardiomyopathy (DCM) is a disease of the myocardium, which causes heart failure and premature death. It has been described in humans and several domestic animals. In the Newfoundland dog, DCM is an autosomal dominant disease with late onset and reduced penetrance. We analyzed 15 candidate genes for their involvement in DCM in the Newfoundland dog. Polymorphic microsatellite markers and single Nucleotide Polymorphisms were genotyped in 4 families of Newfoundland dogs segregating dilated cardiomyopathy for the genes encoding α-cardiac actin (ACTC), caveolin (CAVI), cysteine-rich protein 3 (CSRP3), LIM-domain binding factor 3 (LDB3), desmin (DES), lamin A/C (LMNA), myosin heavy polypeptide 7 (MYH7), delta-sarcoglycan (SGCD), troponin I (TNNTI3), troponin T (TNNT2), alpha-tropomyosin (TPMI), titin (TTN) and vinculin (VCL). A Logarithm of the odds (LOD) score of less than -2.0 in 2-point linkage analysis indicated exclusion of all but 2 genes, encoding CSRP3 and DES. A (LOD) score between -1.5 and -2.0 for CSRP3 and DES makes these genes unlikely causes of DCM in this dog breed. For the phospholamban (PLN) and titin cap (TTN) genes, a direct mutation screening approach was used. DNA sequence analysis of all exons showed no evidence that these genes are involved in DCM in the Newfoundland dog. © The American Genetic Association. 2007. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Wiersma, Anje C. Stabej, Polona Leegwater, Peter A J Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna |
author_facet |
Wiersma, Anje C. Stabej, Polona Leegwater, Peter A J Van Oost, Bernard A. Ollier, William E. Dukes-McEwan, Joanna |
author_sort |
Wiersma, Anje C. |
title |
Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
title_short |
Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
title_full |
Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
title_fullStr |
Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
title_full_unstemmed |
Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog |
title_sort |
evaluation of 15 candidate genes for dilated cardiomyopathy in the newfoundland dog |
publishDate |
2008 |
url |
https://research.manchester.ac.uk/en/publications/85c0501e-3801-49b6-a8f9-92064c4129bd https://doi.org/10.1093/jhered/esm090 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
Wiersma , A C , Stabej , P , Leegwater , P A J , Van Oost , B A , Ollier , W E & Dukes-McEwan , J 2008 , ' Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog ' , Journal of Heredity , vol. 99 , no. 1 , pp. 73-80 . https://doi.org/10.1093/jhered/esm090 |
op_relation |
https://research.manchester.ac.uk/en/publications/85c0501e-3801-49b6-a8f9-92064c4129bd |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1093/jhered/esm090 |
container_title |
Journal of Heredity |
container_volume |
99 |
container_issue |
1 |
container_start_page |
73 |
op_container_end_page |
80 |
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1802646960731062272 |