Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection
Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides...
Published in: | Carbohydrate Polymers |
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Online Access: | https://research.manchester.ac.uk/en/publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 https://doi.org/10.1016/j.carbpol.2016.09.037 |
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ftumanchesterpub:oai:pure.atira.dk:publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 2024-04-28T08:37:55+00:00 Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria L. Corsaro, M. Michela 2017-01-20 https://research.manchester.ac.uk/en/publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 https://doi.org/10.1016/j.carbpol.2016.09.037 eng eng https://research.manchester.ac.uk/en/publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 info:eu-repo/semantics/closedAccess Casillo , A , Parrilli , E , Sannino , F , Mitchell , D E , Gibson , M I , Marino , G , Lanzetta , R , Parrilli , M , Cosconati , S , Novellino , E , Randazzo , A , Tutino , M L & Corsaro , M M 2017 , ' Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : A strategy for cryoprotection ' , Carbohydrate Polymers , vol. 156 , pp. 364-371 . https://doi.org/10.1016/j.carbpol.2016.09.037 exopolymer Colwellia psychrerythraea 34H Alanine decoration NMR spectroscopy cold-adaptation article 2017 ftumanchesterpub https://doi.org/10.1016/j.carbpol.2016.09.037 2024-04-08T14:19:51Z Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4 °C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory effect on ice recrystallization. In-depth NMR and computational analysis suggest a pseudohelicoidal structure which seems to prevent the local tetrahedral order of the water molecules in the first hydration shell, and could be responsible of the inhibition of ice recrystallization. As cell cryopreservation is an essential tool in modern biotechnology and medicine, the observations reported in this paper could pave the way for a biotechnological application of Colwellia EPS. Article in Journal/Newspaper Sea ice The University of Manchester: Research Explorer Carbohydrate Polymers 156 364 371 |
institution |
Open Polar |
collection |
The University of Manchester: Research Explorer |
op_collection_id |
ftumanchesterpub |
language |
English |
topic |
exopolymer Colwellia psychrerythraea 34H Alanine decoration NMR spectroscopy cold-adaptation |
spellingShingle |
exopolymer Colwellia psychrerythraea 34H Alanine decoration NMR spectroscopy cold-adaptation Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria L. Corsaro, M. Michela Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
topic_facet |
exopolymer Colwellia psychrerythraea 34H Alanine decoration NMR spectroscopy cold-adaptation |
description |
Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4 °C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory effect on ice recrystallization. In-depth NMR and computational analysis suggest a pseudohelicoidal structure which seems to prevent the local tetrahedral order of the water molecules in the first hydration shell, and could be responsible of the inhibition of ice recrystallization. As cell cryopreservation is an essential tool in modern biotechnology and medicine, the observations reported in this paper could pave the way for a biotechnological application of Colwellia EPS. |
format |
Article in Journal/Newspaper |
author |
Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria L. Corsaro, M. Michela |
author_facet |
Casillo, Angela Parrilli, Ermenegilda Sannino, Filomena Mitchell, Daniel E. Gibson, Matthew I. Marino, Gennaro Lanzetta, Rosa Parrilli, Michelangelo Cosconati, Sandro Novellino, Ettore Randazzo, Antonio Tutino, Maria L. Corsaro, M. Michela |
author_sort |
Casillo, Angela |
title |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
title_short |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
title_full |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
title_fullStr |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
title_full_unstemmed |
Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:A strategy for cryoprotection |
title_sort |
structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium:a strategy for cryoprotection |
publishDate |
2017 |
url |
https://research.manchester.ac.uk/en/publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 https://doi.org/10.1016/j.carbpol.2016.09.037 |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Casillo , A , Parrilli , E , Sannino , F , Mitchell , D E , Gibson , M I , Marino , G , Lanzetta , R , Parrilli , M , Cosconati , S , Novellino , E , Randazzo , A , Tutino , M L & Corsaro , M M 2017 , ' Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : A strategy for cryoprotection ' , Carbohydrate Polymers , vol. 156 , pp. 364-371 . https://doi.org/10.1016/j.carbpol.2016.09.037 |
op_relation |
https://research.manchester.ac.uk/en/publications/1ef63280-69cf-4bd9-be48-9594bc2d2ea2 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.carbpol.2016.09.037 |
container_title |
Carbohydrate Polymers |
container_volume |
156 |
container_start_page |
364 |
op_container_end_page |
371 |
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1797569160961589248 |