Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack
Cryo-electron tomography (CET) of plunge-frozen whole bacteria and vitreous sections (CETOVIS) were used to revise and expand the structural knowledge of the "Stack", a recently described cytoplasmic structure in the Antarctic bacterium Pseudomonas deceptionensis M1(T). The advantages of b...
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ftumaastrichtcri:oai:cris.maastrichtuniversity.nl:publications/c1e3c0f1-7243-4f5f-bad3-6505db0322d4 2023-05-15T13:37:35+02:00 Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack Delgado, Lidia Martinez, Gema Lopez-Iglesias, Carmen Mercade, Elena 2015-03 https://cris.maastrichtuniversity.nl/en/publications/c1e3c0f1-7243-4f5f-bad3-6505db0322d4 https://doi.org/10.1016/j.jsb.2015.01.008 eng eng info:eu-repo/semantics/openAccess Delgado , L , Martinez , G , Lopez-Iglesias , C & Mercade , E 2015 , ' Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack ' , Journal of Structural Biology , vol. 189 , no. 3 , pp. 220-229 . https://doi.org/10.1016/j.jsb.2015.01.008 Cryo-electron microscopy Cryo-electron tomography Vitreous sections Plunge freezing High-pressure freezing Stacks article 2015 ftumaastrichtcri https://doi.org/10.1016/j.jsb.2015.01.008 2022-07-19T08:59:04Z Cryo-electron tomography (CET) of plunge-frozen whole bacteria and vitreous sections (CETOVIS) were used to revise and expand the structural knowledge of the "Stack", a recently described cytoplasmic structure in the Antarctic bacterium Pseudomonas deceptionensis M1(T). The advantages of both techniques can be complementarily combined to obtain more reliable insights into cells and their components with three-dimensional imaging at different resolutions. Cryo-electron microscopy (Cryo-EM) and CET of frozen-hydrated P. deceptionensis M1(T) cells confirmed that Stacks are found at different locations within the cell cytoplasm, in variable number, separately or grouped together, very close to the plasma membrane (PM) and oriented at different angles (from 35? to 90?) to the PM, thus establishing that they were not artifacts of the previous sample preparation methods. CET of plunge-frozen whole bacteria and vitreous sections verified that each Stack consisted of a pile of oval disc-like subunits, each disc being surrounded by a lipid bilayer membrane and separated from each other by a constant distance with a mean value of 5.2?1.3nm. FM4-64 staining and confocal microscopy corroborated the lipid nature of the membrane of the Stacked discs. Stacks did not appear to be invaginations of the PM because no continuity between both membranes was visible when whole bacteria were analyzed. We are still far from deciphering the function of these new structures, but a first experimental attempt links the Stacks with a given phase of the cell replication process. The Authors. Article in Journal/Newspaper Antarc* Antarctic Maastricht University Research Publications Antarctic The Antarctic Journal of Structural Biology 189 3 220 229 |
institution |
Open Polar |
collection |
Maastricht University Research Publications |
op_collection_id |
ftumaastrichtcri |
language |
English |
topic |
Cryo-electron microscopy Cryo-electron tomography Vitreous sections Plunge freezing High-pressure freezing Stacks |
spellingShingle |
Cryo-electron microscopy Cryo-electron tomography Vitreous sections Plunge freezing High-pressure freezing Stacks Delgado, Lidia Martinez, Gema Lopez-Iglesias, Carmen Mercade, Elena Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
topic_facet |
Cryo-electron microscopy Cryo-electron tomography Vitreous sections Plunge freezing High-pressure freezing Stacks |
description |
Cryo-electron tomography (CET) of plunge-frozen whole bacteria and vitreous sections (CETOVIS) were used to revise and expand the structural knowledge of the "Stack", a recently described cytoplasmic structure in the Antarctic bacterium Pseudomonas deceptionensis M1(T). The advantages of both techniques can be complementarily combined to obtain more reliable insights into cells and their components with three-dimensional imaging at different resolutions. Cryo-electron microscopy (Cryo-EM) and CET of frozen-hydrated P. deceptionensis M1(T) cells confirmed that Stacks are found at different locations within the cell cytoplasm, in variable number, separately or grouped together, very close to the plasma membrane (PM) and oriented at different angles (from 35? to 90?) to the PM, thus establishing that they were not artifacts of the previous sample preparation methods. CET of plunge-frozen whole bacteria and vitreous sections verified that each Stack consisted of a pile of oval disc-like subunits, each disc being surrounded by a lipid bilayer membrane and separated from each other by a constant distance with a mean value of 5.2?1.3nm. FM4-64 staining and confocal microscopy corroborated the lipid nature of the membrane of the Stacked discs. Stacks did not appear to be invaginations of the PM because no continuity between both membranes was visible when whole bacteria were analyzed. We are still far from deciphering the function of these new structures, but a first experimental attempt links the Stacks with a given phase of the cell replication process. The Authors. |
format |
Article in Journal/Newspaper |
author |
Delgado, Lidia Martinez, Gema Lopez-Iglesias, Carmen Mercade, Elena |
author_facet |
Delgado, Lidia Martinez, Gema Lopez-Iglesias, Carmen Mercade, Elena |
author_sort |
Delgado, Lidia |
title |
Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
title_short |
Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
title_full |
Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
title_fullStr |
Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
title_full_unstemmed |
Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack |
title_sort |
cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the stack |
publishDate |
2015 |
url |
https://cris.maastrichtuniversity.nl/en/publications/c1e3c0f1-7243-4f5f-bad3-6505db0322d4 https://doi.org/10.1016/j.jsb.2015.01.008 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Delgado , L , Martinez , G , Lopez-Iglesias , C & Mercade , E 2015 , ' Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack ' , Journal of Structural Biology , vol. 189 , no. 3 , pp. 220-229 . https://doi.org/10.1016/j.jsb.2015.01.008 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.jsb.2015.01.008 |
container_title |
Journal of Structural Biology |
container_volume |
189 |
container_issue |
3 |
container_start_page |
220 |
op_container_end_page |
229 |
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1766094410559258624 |