Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish
The aryl hydrocarbon receptor (AhR) mediates the toxicity of several environmental contaminants, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin, and other halogenated hydrocarbons in vertebrates. This receptor initiates the transcription of several biotransformation enzymes, which in turn are responsible...
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ftulundlup:oai:lup.lub.lu.se:f0b581c3-3226-4784-8847-1056d28b68a4 2023-05-15T15:32:00+02:00 Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn 2003 https://lup.lub.lu.se/record/137376 https://doi.org/10.1016/S0378-1119(02)01178-2 eng eng Elsevier https://lup.lub.lu.se/record/137376 http://dx.doi.org/10.1016/S0378-1119(02)01178-2 pmid:12559581 wos:000181124200020 scopus:0037448608 Gene; 303, pp 197-206 (2003) ISSN: 1879-0038 Biological Sciences Ecology contributiontojournal/article info:eu-repo/semantics/article text 2003 ftulundlup https://doi.org/10.1016/S0378-1119(02)01178-2 2023-02-01T23:27:41Z The aryl hydrocarbon receptor (AhR) mediates the toxicity of several environmental contaminants, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin, and other halogenated hydrocarbons in vertebrates. This receptor initiates the transcription of several biotransformation enzymes, which in turn are responsible for causing severe harm to biological tissue. Here we describe the isolation and complete characterization of the first two AhR genes from the teleost fish Atlantic salmon (Salmo salar). The predicted amino acid sequences contain regions characteristic of other vertebrate AhRs including basic helix-loop-helix (bHLH) and PER-ARNT-SIM (PAS) domains but show little similarity to other vertebrate AhRs across the C-terminal half. Furthermore, they do not contain distinct Q-rich domains as found in the mammalian AhR, which is in line with previously described fish AhR genes. The salmon cDNAs encode 1106 and 1107 putative residues, respectively, approximately 50 amino acids longer than previously characterized AhR genes. Phylogenetic analyses demonstrated that the two salmon AhR sequences cluster within the AhR subfamily of the bHLH-PAS family, in a clade containing fish AhR2 genes. Although the two AbR2 forms are 92% identical at the amino acid level, the distribution of sequence differences and the presence of both forms in 30 tested individuals suggest that they are not allelic but derived from separate loci. Interestingly, they are not orthologs of the rainbow trout (Oncorhynchus mykiss) AhR2alpha and beta genes and the new salmon loci are therefore here designated AhR2gamma and AhR2delta. In line with this, PCR with DNA from rainbow trout revealed a new trout AhR locus that was more similar to the two salmon genes than to the trout AhR2alpha and beta genes, suggesting that the rainbow trout possesses at least three distinct AhR2 genes. The presence of multiple AhR genes in these species is probably a consequence of the genome duplications that occurred in the early evolution of fish and later also specifically in the ... Article in Journal/Newspaper Atlantic salmon Salmo salar Lund University Publications (LUP) Gene 303 197 206 |
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language |
English |
topic |
Biological Sciences Ecology |
spellingShingle |
Biological Sciences Ecology Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
topic_facet |
Biological Sciences Ecology |
description |
The aryl hydrocarbon receptor (AhR) mediates the toxicity of several environmental contaminants, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin, and other halogenated hydrocarbons in vertebrates. This receptor initiates the transcription of several biotransformation enzymes, which in turn are responsible for causing severe harm to biological tissue. Here we describe the isolation and complete characterization of the first two AhR genes from the teleost fish Atlantic salmon (Salmo salar). The predicted amino acid sequences contain regions characteristic of other vertebrate AhRs including basic helix-loop-helix (bHLH) and PER-ARNT-SIM (PAS) domains but show little similarity to other vertebrate AhRs across the C-terminal half. Furthermore, they do not contain distinct Q-rich domains as found in the mammalian AhR, which is in line with previously described fish AhR genes. The salmon cDNAs encode 1106 and 1107 putative residues, respectively, approximately 50 amino acids longer than previously characterized AhR genes. Phylogenetic analyses demonstrated that the two salmon AhR sequences cluster within the AhR subfamily of the bHLH-PAS family, in a clade containing fish AhR2 genes. Although the two AbR2 forms are 92% identical at the amino acid level, the distribution of sequence differences and the presence of both forms in 30 tested individuals suggest that they are not allelic but derived from separate loci. Interestingly, they are not orthologs of the rainbow trout (Oncorhynchus mykiss) AhR2alpha and beta genes and the new salmon loci are therefore here designated AhR2gamma and AhR2delta. In line with this, PCR with DNA from rainbow trout revealed a new trout AhR locus that was more similar to the two salmon genes than to the trout AhR2alpha and beta genes, suggesting that the rainbow trout possesses at least three distinct AhR2 genes. The presence of multiple AhR genes in these species is probably a consequence of the genome duplications that occurred in the early evolution of fish and later also specifically in the ... |
format |
Article in Journal/Newspaper |
author |
Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn |
author_facet |
Hansson, Maria Wittzell, Håkan Persson, Kerstin von Schantz, Torbjörn |
author_sort |
Hansson, Maria |
title |
Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
title_short |
Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
title_full |
Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
title_fullStr |
Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
title_full_unstemmed |
Characterization of two distinct aryl hydrocarbon receptor (AhR2) genes in Atlantic salmon (Salmo salar) and evidence for multiple AhR2 gene lineages in salmonid fish |
title_sort |
characterization of two distinct aryl hydrocarbon receptor (ahr2) genes in atlantic salmon (salmo salar) and evidence for multiple ahr2 gene lineages in salmonid fish |
publisher |
Elsevier |
publishDate |
2003 |
url |
https://lup.lub.lu.se/record/137376 https://doi.org/10.1016/S0378-1119(02)01178-2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Gene; 303, pp 197-206 (2003) ISSN: 1879-0038 |
op_relation |
https://lup.lub.lu.se/record/137376 http://dx.doi.org/10.1016/S0378-1119(02)01178-2 pmid:12559581 wos:000181124200020 scopus:0037448608 |
op_doi |
https://doi.org/10.1016/S0378-1119(02)01178-2 |
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Gene |
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303 |
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197 |
op_container_end_page |
206 |
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1766362492077867008 |