Manufacturing specialized wax esters in plants
Biologically produced wax esters can fulfil different industrial purposes. These functionalities almost drove the sperm whale to extinction from hunting. After the ban on hunting, there is a niche in the global market for biolubricants with properties similar to spermaceti. Wax esters can also serve...
Published in: | Metabolic Engineering |
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Format: | Article in Journal/Newspaper |
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2022
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Online Access: | https://lup.lub.lu.se/record/e63536fc-0d7c-4910-99ce-c6754b022e19 https://doi.org/10.1016/j.ymben.2022.05.005 |
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ftulundlup:oai:lup.lub.lu.se:e63536fc-0d7c-4910-99ce-c6754b022e19 2024-04-28T08:39:48+00:00 Manufacturing specialized wax esters in plants Demski, Kamil Ding, Bao Jian Wang, Hong Lei Tran, Tam N.T. Durrett, Timothy P. Lager, Ida Löfstedt, Christer Hofvander, Per 2022-07 https://lup.lub.lu.se/record/e63536fc-0d7c-4910-99ce-c6754b022e19 https://doi.org/10.1016/j.ymben.2022.05.005 eng eng Elsevier https://lup.lub.lu.se/record/e63536fc-0d7c-4910-99ce-c6754b022e19 http://dx.doi.org/10.1016/j.ymben.2022.05.005 scopus:85130958616 pmid:35598886 Metabolic Engineering; 72, pp 391-402 (2022) ISSN: 1096-7176 Biochemistry and Molecular Biology Inorganic Chemistry Insect sex pheromones Pest control Plant factory Spermaceti Synthetic biology Wax esters contributiontojournal/article info:eu-repo/semantics/article text 2022 ftulundlup https://doi.org/10.1016/j.ymben.2022.05.005 2024-04-09T23:41:38Z Biologically produced wax esters can fulfil different industrial purposes. These functionalities almost drove the sperm whale to extinction from hunting. After the ban on hunting, there is a niche in the global market for biolubricants with properties similar to spermaceti. Wax esters can also serve as a mechanism for producing insect sex pheromone fatty alcohols. Pheromone-based mating disruption strategies are in high demand to replace the toxic pesticides in agriculture and manage insect plagues threatening our food and fiber reserves. In this study we set out to investigate the possibilities of in planta assembly of wax esters, for specific applications, through transient expression of various mix-and-match combinations of genes in Nicotiana benthamiana leaves. Our synthetic biology designs were outlined in order to pivot plant lipid metabolism into producing wax esters with targeted fatty acyl and fatty alcohols moieties. Through this approach we managed to obtain industrially important spermaceti-like wax esters enriched in medium-chain fatty acyl and/or fatty alcohol moieties of wax esters. Via employment of plant codon-optimized moth acyl-CoA desaturases we also managed to capture unusual, unsaturated fatty alcohol and fatty acyl moieties, structurally similar to moth pheromone compounds, in plant-accumulated wax esters. Comparison between outcomes of different experimental designs identified targets for stable transformation to accumulate specialized wax esters and helped us to recognize possible bottlenecks of such accumulation. Article in Journal/Newspaper Sperm whale Lund University Publications (LUP) Metabolic Engineering 72 391 402 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Biochemistry and Molecular Biology Inorganic Chemistry Insect sex pheromones Pest control Plant factory Spermaceti Synthetic biology Wax esters |
spellingShingle |
Biochemistry and Molecular Biology Inorganic Chemistry Insect sex pheromones Pest control Plant factory Spermaceti Synthetic biology Wax esters Demski, Kamil Ding, Bao Jian Wang, Hong Lei Tran, Tam N.T. Durrett, Timothy P. Lager, Ida Löfstedt, Christer Hofvander, Per Manufacturing specialized wax esters in plants |
topic_facet |
Biochemistry and Molecular Biology Inorganic Chemistry Insect sex pheromones Pest control Plant factory Spermaceti Synthetic biology Wax esters |
description |
Biologically produced wax esters can fulfil different industrial purposes. These functionalities almost drove the sperm whale to extinction from hunting. After the ban on hunting, there is a niche in the global market for biolubricants with properties similar to spermaceti. Wax esters can also serve as a mechanism for producing insect sex pheromone fatty alcohols. Pheromone-based mating disruption strategies are in high demand to replace the toxic pesticides in agriculture and manage insect plagues threatening our food and fiber reserves. In this study we set out to investigate the possibilities of in planta assembly of wax esters, for specific applications, through transient expression of various mix-and-match combinations of genes in Nicotiana benthamiana leaves. Our synthetic biology designs were outlined in order to pivot plant lipid metabolism into producing wax esters with targeted fatty acyl and fatty alcohols moieties. Through this approach we managed to obtain industrially important spermaceti-like wax esters enriched in medium-chain fatty acyl and/or fatty alcohol moieties of wax esters. Via employment of plant codon-optimized moth acyl-CoA desaturases we also managed to capture unusual, unsaturated fatty alcohol and fatty acyl moieties, structurally similar to moth pheromone compounds, in plant-accumulated wax esters. Comparison between outcomes of different experimental designs identified targets for stable transformation to accumulate specialized wax esters and helped us to recognize possible bottlenecks of such accumulation. |
format |
Article in Journal/Newspaper |
author |
Demski, Kamil Ding, Bao Jian Wang, Hong Lei Tran, Tam N.T. Durrett, Timothy P. Lager, Ida Löfstedt, Christer Hofvander, Per |
author_facet |
Demski, Kamil Ding, Bao Jian Wang, Hong Lei Tran, Tam N.T. Durrett, Timothy P. Lager, Ida Löfstedt, Christer Hofvander, Per |
author_sort |
Demski, Kamil |
title |
Manufacturing specialized wax esters in plants |
title_short |
Manufacturing specialized wax esters in plants |
title_full |
Manufacturing specialized wax esters in plants |
title_fullStr |
Manufacturing specialized wax esters in plants |
title_full_unstemmed |
Manufacturing specialized wax esters in plants |
title_sort |
manufacturing specialized wax esters in plants |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://lup.lub.lu.se/record/e63536fc-0d7c-4910-99ce-c6754b022e19 https://doi.org/10.1016/j.ymben.2022.05.005 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Metabolic Engineering; 72, pp 391-402 (2022) ISSN: 1096-7176 |
op_relation |
https://lup.lub.lu.se/record/e63536fc-0d7c-4910-99ce-c6754b022e19 http://dx.doi.org/10.1016/j.ymben.2022.05.005 scopus:85130958616 pmid:35598886 |
op_doi |
https://doi.org/10.1016/j.ymben.2022.05.005 |
container_title |
Metabolic Engineering |
container_volume |
72 |
container_start_page |
391 |
op_container_end_page |
402 |
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1797570687451267072 |