Exploring thermostable glycoside hydrolases: Amylases and beta-glucosidases

• Main Author: Turner, Pernilla
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: Department of Biotechnology, Lund University 2007
• Subjects: Industrial Biotechnology; Bioteknik; Biotechnology; Proteiner; beta-glucosidase; thermostability; activity; cyclodextrinase; enzymology; crystallization; enzymologi; expression; glycoside hydrolase; Proteins; transglycosylation; Almond; Iceland
• Online Access: https://lup.lub.lu.se/record/547887

Two putative cyclodextrinases (CDases) were amplified from two moderate thermophiles isolated in Iceland: Anoxybacillus flavithermus and Laceyella sacchari. They were cloned, expressed and characterized. The expression of the former was optimized in E. coli by using a solubility-enhancing tag, NusA,...