Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem
N-15 labeled ammonium, glycine or glutamic acid was injected into subarctic heath soil in situ, with the purpose of investigating how the nitrogen added in these pulses was subsequently utilized and cycled in the ecosystem. We analyzed the acquisition of N-15 label in mycorrhizal and non-mycorrhizal...
Published in: | Plant and Soil |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer
2008
|
Subjects: | |
Online Access: | https://lup.lub.lu.se/record/1305867 https://doi.org/10.1007/s11104-008-9700-7 |
id |
ftulundlup:oai:lup.lub.lu.se:d51d1ca8-cb4c-423a-aa56-29ef0b69f069 |
---|---|
record_format |
openpolar |
spelling |
ftulundlup:oai:lup.lub.lu.se:d51d1ca8-cb4c-423a-aa56-29ef0b69f069 2023-05-15T18:28:21+02:00 Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem Andresen, Louise C. Jonasson, Sven Ström, Lena Michelsen, Anders 2008 https://lup.lub.lu.se/record/1305867 https://doi.org/10.1007/s11104-008-9700-7 eng eng Springer https://lup.lub.lu.se/record/1305867 http://dx.doi.org/10.1007/s11104-008-9700-7 wos:000260961600022 scopus:56349106865 Plant and Soil; 313(1-2), pp 283-295 (2008) ISSN: 0032-079X Physical Geography nitrogen Organic N-15 Mycorrhiza Freeze-thaw cycle Ammonium Amino acid Plant nitrogen uptake Root biomass contributiontojournal/article info:eu-repo/semantics/article text 2008 ftulundlup https://doi.org/10.1007/s11104-008-9700-7 2023-02-01T23:26:59Z N-15 labeled ammonium, glycine or glutamic acid was injected into subarctic heath soil in situ, with the purpose of investigating how the nitrogen added in these pulses was subsequently utilized and cycled in the ecosystem. We analyzed the acquisition of N-15 label in mycorrhizal and non-mycorrhizal plants and in soil microorganisms, in order to reveal probable differences in acquisition patterns between the two functional plant types and between plants and soil microorganisms. Three weeks after the label addition, with the N-15-forms added with same amount of nitrogen per square meter, we analyzed the N-15-enrichment in total soil, in soil K2SO4 (0.5 M) extracts and in the microbial biomass after vacuum-incubation of soil in chloroform and subsequent K2SO4 extraction. Furthermore the N-15-enrichment was analyzed in current years leaves of the dominant plant species sampled three, five and 21 days after label addition. The soil microorganisms had very high N-15 recovery from all the N sources compared to plants. Microorganisms incorporated most N-15 from the glutamic acid source, intermediate amounts of N-15 from the glycine source and least N-15 from the NH4+ source. In contrast to microorganisms, all ten investigated plant species generally acquired more N-15 label from the NH4+ source than from the amino acid sources. Non-mycorrhizal plant species showed higher concentration of N-15 label than mycorrhizal plant species 3 days after labeling, while 21 days after labeling their acquisition of N-15 label from amino acid injection was lower than, and the acquisition of N-15 label from NH4 injection was similar to that of the mycorrhizal species. We conclude that the soil microorganisms were more efficient than plants in acquiring pulses of nutrients which, under natural conditions, occur after e. g. freeze-thaw and dry rewet events, although of smaller size. It also appears that the mycorrhizal plants in the short term may be less efficient than non-mycorrhizal plants in nitrogen acquisition, but in a longer term ... Article in Journal/Newspaper Subarctic Lund University Publications (LUP) Plant and Soil 313 1-2 283 295 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Physical Geography nitrogen Organic N-15 Mycorrhiza Freeze-thaw cycle Ammonium Amino acid Plant nitrogen uptake Root biomass |
spellingShingle |
Physical Geography nitrogen Organic N-15 Mycorrhiza Freeze-thaw cycle Ammonium Amino acid Plant nitrogen uptake Root biomass Andresen, Louise C. Jonasson, Sven Ström, Lena Michelsen, Anders Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
topic_facet |
Physical Geography nitrogen Organic N-15 Mycorrhiza Freeze-thaw cycle Ammonium Amino acid Plant nitrogen uptake Root biomass |
description |
N-15 labeled ammonium, glycine or glutamic acid was injected into subarctic heath soil in situ, with the purpose of investigating how the nitrogen added in these pulses was subsequently utilized and cycled in the ecosystem. We analyzed the acquisition of N-15 label in mycorrhizal and non-mycorrhizal plants and in soil microorganisms, in order to reveal probable differences in acquisition patterns between the two functional plant types and between plants and soil microorganisms. Three weeks after the label addition, with the N-15-forms added with same amount of nitrogen per square meter, we analyzed the N-15-enrichment in total soil, in soil K2SO4 (0.5 M) extracts and in the microbial biomass after vacuum-incubation of soil in chloroform and subsequent K2SO4 extraction. Furthermore the N-15-enrichment was analyzed in current years leaves of the dominant plant species sampled three, five and 21 days after label addition. The soil microorganisms had very high N-15 recovery from all the N sources compared to plants. Microorganisms incorporated most N-15 from the glutamic acid source, intermediate amounts of N-15 from the glycine source and least N-15 from the NH4+ source. In contrast to microorganisms, all ten investigated plant species generally acquired more N-15 label from the NH4+ source than from the amino acid sources. Non-mycorrhizal plant species showed higher concentration of N-15 label than mycorrhizal plant species 3 days after labeling, while 21 days after labeling their acquisition of N-15 label from amino acid injection was lower than, and the acquisition of N-15 label from NH4 injection was similar to that of the mycorrhizal species. We conclude that the soil microorganisms were more efficient than plants in acquiring pulses of nutrients which, under natural conditions, occur after e. g. freeze-thaw and dry rewet events, although of smaller size. It also appears that the mycorrhizal plants in the short term may be less efficient than non-mycorrhizal plants in nitrogen acquisition, but in a longer term ... |
format |
Article in Journal/Newspaper |
author |
Andresen, Louise C. Jonasson, Sven Ström, Lena Michelsen, Anders |
author_facet |
Andresen, Louise C. Jonasson, Sven Ström, Lena Michelsen, Anders |
author_sort |
Andresen, Louise C. |
title |
Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
title_short |
Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
title_full |
Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
title_fullStr |
Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
title_full_unstemmed |
Uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
title_sort |
uptake of pulse injected nitrogen by soil microbes and mycorrhizal and non-mycorrhizal plants in a species-diverse subarctic heath ecosystem |
publisher |
Springer |
publishDate |
2008 |
url |
https://lup.lub.lu.se/record/1305867 https://doi.org/10.1007/s11104-008-9700-7 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Plant and Soil; 313(1-2), pp 283-295 (2008) ISSN: 0032-079X |
op_relation |
https://lup.lub.lu.se/record/1305867 http://dx.doi.org/10.1007/s11104-008-9700-7 wos:000260961600022 scopus:56349106865 |
op_doi |
https://doi.org/10.1007/s11104-008-9700-7 |
container_title |
Plant and Soil |
container_volume |
313 |
container_issue |
1-2 |
container_start_page |
283 |
op_container_end_page |
295 |
_version_ |
1766210799558197248 |