A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications
In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry...
Published in: | Aquacultural Engineering |
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Online Access: | https://lup.lub.lu.se/record/3987186 https://doi.org/10.1016/j.aquaeng.2013.01.004 |
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ftulundlup:oai:lup.lub.lu.se:b7dc57a4-2a03-4aec-b77b-f3018a1d170b 2023-05-15T18:15:51+02:00 A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications Stiller, Kevin Torben Moran, Damian Vanselow, Klaus Heinrich Marxen, Kai Wuertz, Sven Schulz, Carsten 2013 https://lup.lub.lu.se/record/3987186 https://doi.org/10.1016/j.aquaeng.2013.01.004 eng eng Elsevier https://lup.lub.lu.se/record/3987186 http://dx.doi.org/10.1016/j.aquaeng.2013.01.004 wos:000320216100004 scopus:84878111155 Aquacultural Engineering; 55, pp 23-31 (2013) ISSN: 1873-5614 Zoology Ammonia Carbon dioxide Oxygen Respirometry Recirculating aquaculture system Washout effect contributiontojournal/article info:eu-repo/semantics/article text 2013 ftulundlup https://doi.org/10.1016/j.aquaeng.2013.01.004 2023-02-01T23:29:25Z In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry tanks (250 L in volume each) housed animals, and a tenth (without animals) acted as a reference tank. A single measurement unit made sequential measurements of each tank to eliminate the problem of sensor variation associated with multi-probe setups. The accuracy of the analyzers in relation to measurement range was: O-2 = 1%; CO2 < 1%; NH3 = 2%; temperature <= 0.25%; and pH +/- 0.01. Dissolved CO2 was measured using air-water equilibration coupled with non-dispersive infrared detection of carrier gas, and NH3 was quantified using a reagent-based assay and spectophotometric autoanalyzer. Though expensive and not common in aquaculture or physiology research, these two automated metabolite analyzers could operate in both fresh and seawater, and offered high precision and accuracy. We report on the performance of these instruments for aquaculture research in two trials using a freshwater (rainbow trout, Oncorhynchus mykiss) and seawater fish species (turbot, Scophthalmus maximus). One of the main constraints imposed by the sequential measurement of multiple tanks was the measurement frequency of each tank. In the aforementioned system, NH3 analyzes took the longest (12 min), followed by CO2 (7 min), O-2 (6 min), and pH (3 mm). (C) 2013 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Scophthalmus maximus Turbot Lund University Publications (LUP) Aquacultural Engineering 55 23 31 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Zoology Ammonia Carbon dioxide Oxygen Respirometry Recirculating aquaculture system Washout effect |
spellingShingle |
Zoology Ammonia Carbon dioxide Oxygen Respirometry Recirculating aquaculture system Washout effect Stiller, Kevin Torben Moran, Damian Vanselow, Klaus Heinrich Marxen, Kai Wuertz, Sven Schulz, Carsten A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
topic_facet |
Zoology Ammonia Carbon dioxide Oxygen Respirometry Recirculating aquaculture system Washout effect |
description |
In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry tanks (250 L in volume each) housed animals, and a tenth (without animals) acted as a reference tank. A single measurement unit made sequential measurements of each tank to eliminate the problem of sensor variation associated with multi-probe setups. The accuracy of the analyzers in relation to measurement range was: O-2 = 1%; CO2 < 1%; NH3 = 2%; temperature <= 0.25%; and pH +/- 0.01. Dissolved CO2 was measured using air-water equilibration coupled with non-dispersive infrared detection of carrier gas, and NH3 was quantified using a reagent-based assay and spectophotometric autoanalyzer. Though expensive and not common in aquaculture or physiology research, these two automated metabolite analyzers could operate in both fresh and seawater, and offered high precision and accuracy. We report on the performance of these instruments for aquaculture research in two trials using a freshwater (rainbow trout, Oncorhynchus mykiss) and seawater fish species (turbot, Scophthalmus maximus). One of the main constraints imposed by the sequential measurement of multiple tanks was the measurement frequency of each tank. In the aforementioned system, NH3 analyzes took the longest (12 min), followed by CO2 (7 min), O-2 (6 min), and pH (3 mm). (C) 2013 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Stiller, Kevin Torben Moran, Damian Vanselow, Klaus Heinrich Marxen, Kai Wuertz, Sven Schulz, Carsten |
author_facet |
Stiller, Kevin Torben Moran, Damian Vanselow, Klaus Heinrich Marxen, Kai Wuertz, Sven Schulz, Carsten |
author_sort |
Stiller, Kevin Torben |
title |
A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
title_short |
A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
title_full |
A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
title_fullStr |
A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
title_full_unstemmed |
A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications |
title_sort |
novel respirometer for online detection of metabolites in aquaculture research: evaluation and first applications |
publisher |
Elsevier |
publishDate |
2013 |
url |
https://lup.lub.lu.se/record/3987186 https://doi.org/10.1016/j.aquaeng.2013.01.004 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Aquacultural Engineering; 55, pp 23-31 (2013) ISSN: 1873-5614 |
op_relation |
https://lup.lub.lu.se/record/3987186 http://dx.doi.org/10.1016/j.aquaeng.2013.01.004 wos:000320216100004 scopus:84878111155 |
op_doi |
https://doi.org/10.1016/j.aquaeng.2013.01.004 |
container_title |
Aquacultural Engineering |
container_volume |
55 |
container_start_page |
23 |
op_container_end_page |
31 |
_version_ |
1766189078528655360 |