Solvent-free enzymatic synthesis of fatty alkanolamides
An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to...
Published in: | Biotechnology and Bioengineering |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
John Wiley & Sons Inc.
2007
|
Subjects: | |
Online Access: | https://lup.lub.lu.se/record/659702 https://doi.org/10.1002/bit.21258 |
id |
ftulundlup:oai:lup.lub.lu.se:b4e61ac2-0dc7-44de-8cb5-307c8c83b98f |
---|---|
record_format |
openpolar |
spelling |
ftulundlup:oai:lup.lub.lu.se:b4e61ac2-0dc7-44de-8cb5-307c8c83b98f 2023-05-15T13:50:27+02:00 Solvent-free enzymatic synthesis of fatty alkanolamides Tufvesson, Pär Annerling, Annika Hatti-Kaul, Rajni Adlercreutz, Dietlind 2007 https://lup.lub.lu.se/record/659702 https://doi.org/10.1002/bit.21258 eng eng John Wiley & Sons Inc. https://lup.lub.lu.se/record/659702 http://dx.doi.org/10.1002/bit.21258 wos:000246491700002 scopus:34249812929 Biotechnology and Bioengineering; 97(3), pp 447-453 (2007) ISSN: 1097-0290 Industrial Biotechnology immobilized lipase ethanolamine amide biosurfactant contributiontojournal/article info:eu-repo/semantics/article text 2007 ftulundlup https://doi.org/10.1002/bit.21258 2023-02-01T23:28:34Z An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented. Article in Journal/Newspaper Antarc* Antarctica Lund University Publications (LUP) Biotechnology and Bioengineering 97 3 447 453 |
institution |
Open Polar |
collection |
Lund University Publications (LUP) |
op_collection_id |
ftulundlup |
language |
English |
topic |
Industrial Biotechnology immobilized lipase ethanolamine amide biosurfactant |
spellingShingle |
Industrial Biotechnology immobilized lipase ethanolamine amide biosurfactant Tufvesson, Pär Annerling, Annika Hatti-Kaul, Rajni Adlercreutz, Dietlind Solvent-free enzymatic synthesis of fatty alkanolamides |
topic_facet |
Industrial Biotechnology immobilized lipase ethanolamine amide biosurfactant |
description |
An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented. |
format |
Article in Journal/Newspaper |
author |
Tufvesson, Pär Annerling, Annika Hatti-Kaul, Rajni Adlercreutz, Dietlind |
author_facet |
Tufvesson, Pär Annerling, Annika Hatti-Kaul, Rajni Adlercreutz, Dietlind |
author_sort |
Tufvesson, Pär |
title |
Solvent-free enzymatic synthesis of fatty alkanolamides |
title_short |
Solvent-free enzymatic synthesis of fatty alkanolamides |
title_full |
Solvent-free enzymatic synthesis of fatty alkanolamides |
title_fullStr |
Solvent-free enzymatic synthesis of fatty alkanolamides |
title_full_unstemmed |
Solvent-free enzymatic synthesis of fatty alkanolamides |
title_sort |
solvent-free enzymatic synthesis of fatty alkanolamides |
publisher |
John Wiley & Sons Inc. |
publishDate |
2007 |
url |
https://lup.lub.lu.se/record/659702 https://doi.org/10.1002/bit.21258 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Biotechnology and Bioengineering; 97(3), pp 447-453 (2007) ISSN: 1097-0290 |
op_relation |
https://lup.lub.lu.se/record/659702 http://dx.doi.org/10.1002/bit.21258 wos:000246491700002 scopus:34249812929 |
op_doi |
https://doi.org/10.1002/bit.21258 |
container_title |
Biotechnology and Bioengineering |
container_volume |
97 |
container_issue |
3 |
container_start_page |
447 |
op_container_end_page |
453 |
_version_ |
1766253508011491328 |